Zobrazeno 1 - 10
of 15
pro vyhledávání: '"Ki-Joo Sung"'
Publikováno v:
PLoS ONE, Vol 12, Iss 3, p e0173479 (2017)
Exchange of components such as particles and cells in droplets is important and highly desired in droplet microfluidic assays, and many current technologies use electrical or magnetic fields to accomplish this process. Bead-based microfluidic techniq
Externí odkaz:
https://doaj.org/article/363f07ce92dc44698ee5393684b30cd9
Autor:
Huan Jia, Nazirulmubin Abdul Moomen, Jeanette Leong, Patthara Kongsuphol, Zhi Feng Sherman Lim, Carmen Sze Min Pui, Yuxuan Tan, Ki-Joo Sung, Hadley D. Sikes
Publikováno v:
Sensors & Diagnostics. 2:627-631
Protein engineering approach enables effective immobilisation of thermostable affinity proteins onto polystyrene materials.
Publikováno v:
ACS Applied Bio Materials. 4:392-398
Whatman No. 1 chromatography paper is widely used as a substrate for cellulose-based immunoassays. The immobilized proteins are used to capture target biomarkers for detection. However, alternative paper substrates may facilitate mass production of i
Publikováno v:
The Analyst. 145:2515-2519
Binding protein scaffolds, such as rcSso7d, have been investigated for use in diagnostic tests; however, the functional performance of rcSso7d has not yet been studied in comparison to antibodies. Here, we assessed the analyte-binding capabilities of
Autor:
Eric A. Miller, Hui Qi Aw-Yong, Isabel G. Kaspriskie, Subha Baniya, Karl Pang-Yeo, Farah M. Kabir, Vivian Tay, Patthara Kongsuphol, Ki-Joo Sung, Yuxuan Tan, Hadley D. Sikes
Publikováno v:
ACS Combinatorial Science. 22:49-60
Many biotechnological applications require the simultaneous binding of affinity reagents to nonoverlapping target epitopes, the most prominent example being sandwich immunoassays. Typically, affini...
Autor:
Seunghyeon Kim, Mohsan Saeed, Huan Jia, Emma H. Yee, Hadley D. Sikes, Ki-Joo Sung, Yining Hao, Deniz Yüksel Yurt, Eric A. Miller, Dousabel M Y Tay, Joseph M. Johnson, Charles R. Mace
Publikováno v:
ChemRxiv
article-version (number) 1
article-version (status) pre
article-version (number) 1
article-version (status) pre
The ongoing COVID-19 pandemic has clearly established how vital rapid, widely accessible diagnostic tests are in controlling infectious diseases and how difficult and slow it is to scale existing technologies. Here, we demonstrate the use of the rapi
Publikováno v:
Molecular Systems Design & Engineering. 3:877-882
The hyperthermostable binding scaffold, rcSso7d, has recently been studied for use in in vitro diagnostic tests but has not yet been demonstrated as a soluble reporter protein. Here, we investigated several rcSso7d protein constructs and biotin conju
Autor:
Shuai Nie, Carmen I. Tobos, Ki-Joo Sung, Seunghyeon Kim, Susan Yan, Joseph M. Johnson, David C. Duffy, Hadley D. Sikes, Bradley Rice, Scott Douglas, David M. Rissin
Publikováno v:
Journal of immunological methods. 474
We have characterized the sensitivity and kinetics of a multiplex immunoassay system based on detection of chemiluminescence (CL) at arrays of antibodies. This enzyme-linked immunosorbent assay (ELISA) was based on the spotting of different antibodie
Autor:
Hadley D. Sikes, Ki-Joo Sung, Eric A. Miller, Qifan Zhang, Lukas Zeininger, Kosuke Yoshinaga, Timothy M. Swager
Publikováno v:
ACS Sens
A new class of Janus emulsion agglutination assay is reported for the detection of interfacial protein-protein interactions. Janus emulsion droplets are functionalized with a thermally stable, antigen binding protein rcSso7d variant (rcSso7d-ZNS1) fo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0de60fb4d57253f53a23fd17bfa6f586
https://europepmc.org/articles/PMC7255051/
https://europepmc.org/articles/PMC7255051/
Publikováno v:
PLoS ONE
PLoS ONE, Vol 12, Iss 3, p e0173479 (2017)
PLoS
PLoS ONE, Vol 12, Iss 3, p e0173479 (2017)
PLoS
Exchange of components such as particles and cells in droplets is important and highly desired in droplet microfluidic assays, and many current technologies use electrical or magnetic fields to accomplish this process. Bead-based microfluidic techniq