Zobrazeno 1 - 10
of 21
pro vyhledávání: '"Khalid K, Alam"'
Autor:
Khalid K. Alam, Jonathan L. Chang, Margaret J. Lange, Phuong D.M. Nguyen, Andrew W. Sawyer, Donald H. Burke
Publikováno v:
Molecular Therapy: Nucleic Acids, Vol 13, Iss , Pp 605-619 (2018)
Aptamer selections often yield distinct subpopulations, each with unique phenotypes that can be leveraged for specialized applications. Although most selections aim to attain ever higher specificity, we sought to identify aptamers that recognize incr
Externí odkaz:
https://doaj.org/article/21625fee9d3f4cffbf55bb529e64a415
Autor:
David Porciani, Leah N. Cardwell, Kwaku D. Tawiah, Khalid K. Alam, Margaret J. Lange, Mark A. Daniels, Donald H. Burke
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-13 (2018)
Large RNAs and ribonucleoprotein complexes have shown potential as novel therapeutic agents, but their targeted delivery to cells is still challenging. Here the authors present a modular aptamer nanostructure for intracellular delivery of RNAs up to
Externí odkaz:
https://doaj.org/article/ffee912c372c4f2bb7d08733a0e28329
Publikováno v:
Nature Chemical Biology. 18:385-393
Cell-free biosensors are powerful platforms for monitoring human and environmental health. Here, we expand their capabilities by interfacing them with toehold-mediated strand displacement circuits, a dynamic DNA nanotechnology that enables molecular
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::46f5df22bf23a8c8e1de89df4917de9e
https://doi.org/10.1038/s41589-021-00962-9
https://doi.org/10.1038/s41589-021-00962-9
Combinatorial selections are powerful strategies for identifying biopolymers with specific biological, biomedical, or chemical characteristics. Unfortunately, most available software tools for high-throughput sequencing analysis have high entrance ba
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6bb1c00973467f1b7f3cc17d7495ea2d
https://doi.org/10.1101/2022.04.27.489774
https://doi.org/10.1101/2022.04.27.489774
Publikováno v:
RNA
Nucleic acid aptamers can be chemically modified to enhance function, but modifying previously selected aptamers can have nontrivial structural and functional consequences. We present a reselection strategy to evaluate the impact of several modificat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0d76abd1fdc8a5c7eaad6f37017cf0ea
https://doi.org/10.1261/rna.077008.120
https://doi.org/10.1261/rna.077008.120
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2433
ROSALIND (RNA Output Sensors Activated by Ligand Induction) is an in vitro biosensing system that detects small molecules using regulated transcription reactions. It consists of three key components: (1) RNA polymerases, (2) allosteric protein transc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::a1997401c5089d3e08fb1bf1222e7ed1
https://pubmed.ncbi.nlm.nih.gov/34985754
https://pubmed.ncbi.nlm.nih.gov/34985754
Publikováno v:
Cell-Free Gene Expression ISBN: 9781071619971
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a67b1a7525a1484d72b6afa41b478592
https://doi.org/10.1007/978-1-0716-1998-8_20
https://doi.org/10.1007/978-1-0716-1998-8_20
Autor:
Srivatsan Raman, Adam D. Silverman, Khalid K. Alam, Erik Iverson, Michael C. Jewett, Xiangyang Liu, Julius B. Lucks
Publikováno v:
ACS Synth Biol
Rapid molecular biosensing is an emerging application area for synthetic biology. Here, we engineer a portable biosensor for cyanuric acid (CYA), an analyte of interest for human and environmental health, using a LysR-type transcription regulator (LT
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a9d7d5c1decc82bef11c47ffa3e1eb1f
https://doi.org/10.1021/acssynbio.9b00348
https://doi.org/10.1021/acssynbio.9b00348
Publikováno v:
Molecular Therapy: Nucleic Acids, Vol 4, Iss C (2015)
High-throughput sequence (HTS) analysis of combinatorial selection populations accelerates lead discovery and optimization and offers dynamic insight into selection processes. An underlying principle is that selection enriches high-fitness sequences
Externí odkaz:
https://doaj.org/article/585fd37d641f492c8f349de2cadf3437
Autor:
Jonathan L. Chang, Donald H. Burke, Phuong D.M. Nguyen, Margaret J. Lange, Khalid K. Alam, Andrew W. Sawyer
Publikováno v:
Molecular Therapy: Nucleic Acids, Vol 13, Iss, Pp 605-619 (2018)
Molecular Therapy. Nucleic Acids
Molecular Therapy. Nucleic Acids
Aptamer selections often yield distinct subpopulations, each with unique phenotypes that can be leveraged for specialized applications. Although most selections aim to attain ever higher specificity, we sought to identify aptamers that recognize incr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2958b555a09690cea675a0910fc226d2
http://www.sciencedirect.com/science/article/pii/S2162253118302816
http://www.sciencedirect.com/science/article/pii/S2162253118302816