Hypocrates is a genetically encoded fluorescent biosensor for (pseudo)hypohalous acids and their derivatives

Autor: Alexander I. Kostyuk, Maria-Armineh Tossounian, Anastasiya S. Panova, Marion Thauvin, Roman I. Raevskii, Daria Ezeriņa, Khadija Wahni, Inge Van Molle, Anastasia D. Sergeeva, Didier Vertommen, Andrey Yu. Gorokhovatsky, Mikhail S. Baranov, Sophie Vriz, Joris Messens, Dmitry S. Bilan, Vsevolod V. Belousov

Publikováno v: Nature Communications, Vol 13, Iss 1, Pp 1-17 (2022)

There are a lack of tools to study the dynamics of (pseudo)hypohalous acids in live cells. Here the authors report a genetically encoded fluorescent biosensor, Hypocrates, for (pseudo)hypohalous acids and their derivatives which they use in cells and

Externí odkaz: https://doaj.org/article/9229963fe3634d69b8cb16d786e0e272

Prdx1 Interacts with ASK1 upon Exposure to H2O2 and Independently of a Scaffolding Protein

Autor: Trung Nghia Vo, Julia Malo Pueyo, Khadija Wahni, Daria Ezeriņa, Jesalyn Bolduc, Joris Messens

Publikováno v: Antioxidants, Vol 10, Iss 7, p 1060 (2021)

Hydrogen peroxide (H2O2) is a key redox signaling molecule that selectively oxidizes cysteines on proteins. It can accomplish this even in the presence of highly efficient and abundant H2O2 scavengers, peroxiredoxins (Prdxs), as it is the Prdxs thems

Externí odkaz: https://doaj.org/article/3650ef3609724c7a8a357e95584425d7

Bifunctional Chloroplastic DJ-1B from Arabidopsis thaliana is an Oxidation-Robust Holdase and a Glyoxalase Sensitive to H2O2

Autor: Aleksandra Lewandowska, Trung Nghia Vo, Thuy-Dung Ho Nguyen, Khadija Wahni, Didier Vertommen, Frank Van Breusegem, David Young, Joris Messens

Publikováno v: Antioxidants, Vol 8, Iss 1, p 8 (2019)

Members of the DJ-1 protein family are multifunctional enzymes whose loss increases the susceptibility of the cell to oxidative stress. However, little is known about the function of the plant DJ-1 homologs. Therefore, we analyzed the effect of oxida

Externí odkaz: https://doaj.org/article/5784543e71d7488c9f873ec47a579f0f

Functional and structural characterization of Hypocrates, a genetically encoded biosensor for hypochlorite

Autor: Maria-Armineh Tossounian, Alexander Kostyuk, Anastasiya Panova, Marion Thauvin, Khadija Wahni, Inge Van Molle, Roman Raevskii, Mikhail Baranov, Sophie Vriz, Dmitry Bilan, Vsevolod Belousov, Joris Messens

Publikováno v: Free Radical Biology and Medicine. 177:S94-S95

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::171cdd2e1b05603734a328b091dae398
https://doi.org/10.1016/j.freeradbiomed.2021.08.130

Charge Interactions in a Highly Charge-Depleted Protein

Autor: Yong Wang, Casper Højgaard, David Young, Joris Messens, Kristoffer E. Johansson, Jakob R. Winther, Kresten Lindorff-Larsen, Stefan Hervø-Hansen, Kaare Teilum, Khadija Wahni

Publikováno v: Hervø-Hansen, S, Højgaard, C, Johansson, K E, Wang, Y, Wahni, K, Young, D, Messens, J, Teilum, K, Lindorff-Larsen, K & Winther, J R 2021, ' Charge Interactions in a Highly Charge-Depleted Protein ', Journal of the American Chemical Society, vol. 143, no. 6, pp. 2500-2508 . https://doi.org/10.1021/jacs.0c10789

Interactions between charged residues are difficult to study because of the complex network of interactions found in most proteins. We have designed a purposely simple system to investigate this problem by systematically introducing individual and pa

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8cd3790dbd35442f8af0c67286c525e7
https://pubmed.ncbi.nlm.nih.gov/33529004