Zobrazeno 1 - 5
of 5
pro vyhledávání: '"Kexue Liang"'
Publikováno v:
Fermentation, Vol 10, Iss 7, p 352 (2024)
Monoclonal antibodies (mAbs) used in biomedical research and therapeutic applications are primarily produced by Chinese hamster ovary (CHO) cells via fed-batch culture. The growing need for elevated quantities of biologics mandates the continual opti
Externí odkaz:
https://doaj.org/article/caba93abee264836bd6c64ce2f2f6e11
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 11 (2023)
The perfusion medium is critical in maintaining high cell concentration in cultures for the production of monoclonal antibody by Chinese hamster ovary cells. In this study, the effects of perfusion culture strategies when using different media on the
Externí odkaz:
https://doaj.org/article/c8c5ee72e8274c7f9c0323a431b39f58
Publikováno v:
Applied Biochemistry and Biotechnology. 176:1964-1974
In production of porcine interferon α (pIFN-α) by Pichia pastoris, improper glycerol feeding strategy leads to ethanol accumulation in the last stage of growth phase. In the present study, taking two runs with low ethanol accumulation under 2 g/L a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::98a98a96ef65a1344cd870c4a5874563
https://doi.org/10.1007/s12010-015-1693-0
https://doi.org/10.1007/s12010-015-1693-0
Autor:
Guoli Hou, Ding Jian, Chunling Zhang, Zhen Li, Kexue Liang, Minjie Gao, Chunhua Li, Zhongping Shi, Jianping Ni
Publikováno v:
Journal of Biotechnology. 177:35-44
Porcine circovirus Cap protein production by P. pastoris with strong AOX promoter suffered with the problems with traditional pure methanol induction: (1) inefficient methanol metabolism; (2) extensive oxygen supply load; (3) difficulty in stable DO
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3523215f65249d609bdc168d12be3e1e
https://doi.org/10.1016/j.jbiotec.2014.02.018
https://doi.org/10.1016/j.jbiotec.2014.02.018
Publikováno v:
Journal of Chemical Technology & Biotechnology. 89:1948-1953
BACKGROUND Achievement of very high cell concentration is the prerequisite for enhanced porcine interferon-α (pIFN-α) production by Pichia pastoris, but it is closely associated with severe ethanol accumulation, leading to instability in pIFN-α ex
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9aebf2f3021fd160c8c458e2b32509ef
https://doi.org/10.1002/jctb.4281
https://doi.org/10.1002/jctb.4281