Zobrazeno 1 - 10
of 21
pro vyhledávání: '"Kevin T. Zhao"'
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-8 (2019)
Inefficient delivery of proteins into mammalian cells limits their use in research and therapy. Here, the authors discover that ProTα, a small, intrinsically disordered human protein can facilitate efficient cationic lipid-mediated protein delivery
Externí odkaz:
https://doaj.org/article/c835099de4814592bcbecee8ffae3aee
Autor:
Mandana Arbab, Zaneta Matuszek, Kaitlyn M. Kray, Ailing Du, Gregory A. Newby, Anton J. Blatnik, Aditya Raguram, Michelle F. Richter, Kevin T. Zhao, Jonathan M. Levy, Max W. Shen, W. David Arnold, Dan Wang, Jun Xie, Guangping Gao, Arthur H. M. Burghes, David R. Liu
Publikováno v:
Science. 380
Spinal muscular atrophy (SMA), the leading genetic cause of infant mortality, arises from survival motor neuron (SMN) protein insufficiency resulting from SMN1 loss. Approved therapies circumvent endogenous SMN regulation and require repeated dosing
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::da790fc009f5f996838b159db859f45f
https://doi.org/10.1126/science.adg6518
https://doi.org/10.1126/science.adg6518
Autor:
Vedagopuram Sreekanth, Max Jan, Kevin T. Zhao, Donghyun Lim, Jessie R. Davis, Marie McConkey, Veronica Kovalcik, Sam Barkal, Benjamin K. Law, James Fife, Ruilin Tian, Michael E. Vinyard, Basheer Becerra, Martin Kampmann, Richard I. Sherwood, Luca Pinello, David R. Liu, Benjamin L. Ebert, Amit Choudhary
Publikováno v:
bioRxiv
Cas9 is a programmable nuclease that has furnished transformative technologies, including base editors and transcription modulators (e.g., CRISPRi/a), but several applications of these technologies, including therapeutics, mandatorily require precisi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e30fbb23b8aa817b820edbea0ccfe0e9
https://doi.org/10.1101/2023.03.12.531757
https://doi.org/10.1101/2023.03.12.531757
Autor:
Haoxin Li, Jarrett R. Remsberg, Sang Joon Won, Kevin T. Zhao, Tony P. Huang, Bingwen Lu, Gabriel M. Simon, David R. Liu, Benjamin F. Cravatt
Chemical probes are lacking for most human proteins. Covalent chemistry represents an attractive strategy for expanding the ligandability of the proteome, and chemical proteomics has revealed numerous electrophile-reactive cysteines on diverse protei
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c87ef0d73954efc03eb0f4ae239f9fc2
https://doi.org/10.1101/2022.11.17.516964
https://doi.org/10.1101/2022.11.17.516964
Autor:
Jonathan Yen, Gregory A. Newby, Kalin Mayberry, Akshay Sharma, Michelle Richter, Thiyagaraj Mayuranathan, Kevin T. Zhao, Cicera R. Lazzarotto, Christophe Lechauve, Theodosia A. Kalfa, Elizabeth Thaman, Luke W. Koblan, Shondra M. Pruett-Miller, Heather Sheppard-Tillman, David R. Liu, Mitchell J. Weiss, Shengdar Q. Tsai, John F. Tisdale, Kaitly J. Woodard, Yoonjeong Jang, Christopher J. Podracky, Kelcee A. Everette, Shannon M. Miller, Tina Wang, Shaina N. Porter, Anton P. McCaffrey, Jordana M. Henderson, Yichao Li, Yu Yao
Publikováno v:
Nature
Sickle cell disease (SCD) is caused by a mutation in the β-globin gene HBB1. We used a custom adenine base editor (ABE8e-NRCH)2,3 to convert the SCD allele (HBBS) into Makassar β-globin (HBBG), a non-pathogenic variant4,5. Ex vivo delivery of mRNA
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e1b4a4d36b9c89ce3b3332d5314b6d67
https://doi.org/10.1038/s41586-021-03609-w
https://doi.org/10.1038/s41586-021-03609-w
Publikováno v:
National Science Open. :20220052
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::fcee258fba853f3e55c6de41b8f96795
https://doi.org/10.1360/nso/20220052
https://doi.org/10.1360/nso/20220052
Autor:
Kevin T. Zhao, Jennifer A. Doudna, Luke W. Koblan, Elliot Eton, Christine D. Wilson, Audrone Lapinaite, Gregory A. Newby, Benjamin W. Thuronyi, David R. Liu, Michelle Richter, Daniel E. Bauer, Jing Zeng
Publikováno v:
Nature biotechnology
Applications of adenine base editors (ABEs) have been constrained by the limited compatibility of the deoxyadenosine deaminase component with Cas homologs other than SpCas9. We evolved the deaminase component of ABE7.10 using phage-assisted non-conti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e3abb1223ffa80507addfe37122e5b57
https://doi.org/10.1038/s41587-020-0453-z
https://doi.org/10.1038/s41587-020-0453-z
Publikováno v:
Nature chemical biology. 17(11)
Directed evolution can generate proteins with tailor-made activities. However, full-length genotypes, their frequencies and fitnesses are difficult to measure for evolving gene-length biomolecules using most high-throughput DNA sequencing methods, as
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::499c491df1b57f3a6d1a6fbdeff5ca52
https://pubmed.ncbi.nlm.nih.gov/34635842
https://pubmed.ncbi.nlm.nih.gov/34635842
Autor:
Michelle Richter, Kevin T. Zhao, Enrique Lin-Shiao, Jennifer A. Doudna, Peter A. Beal, Gavin J. Knott, Cody M. Palumbo, Audrone Lapinaite, David R. Liu
Publikováno v:
Science
Secrets of a fast base editor CRISPR-Cas9 base editors comprise RNA-guided Cas proteins fused to an enzyme that can deaminate a DNA nucleoside. No natural enzyme deaminates adenine in DNA, and so a breakthrough came when a natural transfer RNA deamin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c683ba9ba948abc99dd54f13e4180901
https://pubmed.ncbi.nlm.nih.gov/32732424
https://pubmed.ncbi.nlm.nih.gov/32732424
Autor:
Kevin T. Zhao, Jonathan M. Levy, David R. Liu, Y. Bill Kim, Alexis C. Komor, Michael S. Packer
Publikováno v:
Nature biotechnology, vol 35, iss 4
Nature biotechnology
Nature biotechnology
Base editing is a recently developed approach to genome editing that uses a fusion protein containing a catalytically defective Streptococcus pyogenes Cas9, a cytidine deaminase, and an inhibitor of base excision repair to induce programmable, single
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::677e4ab0c5137170e29ca62de7b26fef
https://doi.org/10.1038/nbt.3803
https://doi.org/10.1038/nbt.3803