Výsledky vyhledávání - "Kevin R. Rupprecht"

Development of a dot-blot assay for screening monoclonal antibodies to low-molecular-mass drugs

Autor: Yon-Yih Chen, Rad K. Nair, Sushil D. Rege, Larissa Coombs Harwick, Gangamani S. Beligere, Jonathan Grote, Kevin R. Rupprecht, Jeffrey R. Fishpaugh, Zhen Lin

Publikováno v: Analytical Biochemistry. 407:160-164

Dot-blot is a versatile and simple analysis to perform. We adapted this method as a simple identity test for monoclonal antibodies to a number of small compounds: three transplant drugs, an anticonvulsant, a steroid, an anticancer drug, and an antibi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c5531d4e68f1b40d0c952ca01f957086
https://doi.org/10.1016/j.ab.2010.08.003

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A precise spectrophotometric method for measuring sodium dodecyl sulfate concentration

Autor: Tracey D. Rae, Jeffrey R. Fishpaugh, Ewa Z. Lang, Kevin R. Rupprecht, Svetoslava D. Gregory, Janet M. Bergsma

Publikováno v: Analytical biochemistry. 486

Sodium dodecyl sulfate (SDS) is used to denature and solubilize proteins, especially membrane and other hydrophobic proteins. A quantitative method to determine the concentration of SDS using the dye Stains-All is known. However, this method lacks th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e7571b2ac43ecb4d170d9772cf0d7a9d
https://pubmed.ncbi.nlm.nih.gov/26150094

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Structural characterization of glycoprotein NGAL, an early predictive biomarker for acute kidney injury

Autor: Kevin R. Rupprecht, Frank C. Grenier, Cheng Zhao, Carol Ramsay, Ryan F. Workman, Jeffrey R. Fishpaugh, Panfilo Ozaeta

Publikováno v: Carbohydrate research. 345(15)

Neutrophil gelatinase-associated lipocalin (NGAL) is a promising new renal biomarker that can reduce the time to diagnose acute kidney injury (AKI). There is little information available about complex glycans on NGAL. Detailed structural characteriza

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a829bf6e30d30873755f98d63d748e18
https://pubmed.ncbi.nlm.nih.gov/20800224

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Development of an Abbott ARCHITECT cyclosporine immunoassay without metabolite cross-reactivity

Autor: David M. Finley, Thomas G. Spring, Helen Xie, Kevin R. Rupprecht, Sergey Y. Tetin, Beth C. Trudeau, Joan E. Peart, Sylvia C. Saldana, Elaine Brate, Shelley R. Holets-McCormack, Carol Ramsay, Jonathan Grote, Phil Wang, Pan F. Ozaeta, David Pacenti, Ryan E. Piktel

Publikováno v: Clinical biochemistry. 43(13-14)

Objective : We investigated the mechanism by which the ARCHITECT cyclosporine (CsA) chemiluminescent microparticle immunoassay (CMIA) eliminates cross-reactivity to CsA metabolites AM1 and AM9, despite its use of a monoclonal antibody which shows cro

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8bcf234d062071b3a3524ede36d18370
https://pubmed.ncbi.nlm.nih.gov/20599875

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Microheterogeneous monoclonal antibody subspecies with differential hepatitis C virus core antigen binding properties identified by SEC-HPLC

Autor: Suresh M. Desai, Cheng Zhao, Bill J. Zeck, Carol S. Ramsay, Qiaoqiao Ruan, A. Scott Muerhoff, Kevin R. Rupprecht

Publikováno v: Journal of immunological methods. 345(1-2)

A monoclonal antibody directed against the core protein of hepatitis C virus was characterized for its utility in a sandwich antigen immunoassay wherein the mAb was used as the conjugate. Analysis of unconjugated and acridinium-conjugated monoclonal

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b59ec9e882839e7bf7798746e57d6f0b
https://pubmed.ncbi.nlm.nih.gov/19375428

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