Detection ofP. malariaeusing a new rapid isothermal amplification lateral flow assay

Autor: Ashenafi Assefa, Kevin K. Wamae, Chris M. Hennelly, Billy Ngasala, Meredith Muller, Albert Kalonji, Fernandine Phanzu, Clark H. Cunningham, Jessica T. Lin, Jonathan B. Parr

P. malariaeis found worldwide and causes chronic parasitism in its human hosts. We developed aP. malariae (Pm)diagnostic assay that uses rapid, isothermal recombinase polymerase amplification (RPA) and lateral-flow-strip detection. Using 18S rRNA pla

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::9b8ecca1fd9a182f12b951ad432831bd
https://doi.org/10.1101/2023.02.26.23286371

Application of a new highly multiplexed amplicon sequencing tool to evaluate Plasmodium falciparum antimalarial resistance and relatedness in individual and pooled samples from Dschang, Cameroon.

Autor: Sadler JM; Institute for Global Health and Infectious Diseases, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America., Simkin A; Department of Pathology and Laboratory Medicine, Warren Alpert Medical School, Brown University, Providence, Rhode Island., Tchuenkam VPK; Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, West Region, Cameroon., Gyuricza IG; Institute for Global Health and Infectious Diseases, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America.; Curriculum in Genetics and Molecular Biology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America., Fola AA; Department of Pathology and Laboratory Medicine, Warren Alpert Medical School, Brown University, Providence, Rhode Island., Wamae K; KEMRI-Wellcome Trust Research Programme, Kilifi, Kenya., Assefa A; Institute for Global Health and Infectious Diseases, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America.; Ethiopian Public Health Institute, Addis Ababa, Ethiopia., Niaré K; Department of Pathology and Laboratory Medicine, Warren Alpert Medical School, Brown University, Providence, Rhode Island., Thwai K; Institute for Global Health and Infectious Diseases, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America., White SJ; Institute for Global Health and Infectious Diseases, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America., Moss WJ; Department of Epidemiology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland, United States of America., Dinglasan RR; Emerging Pathogens Institute, University of Florida, Gainesville, Florida, United States of America., Nsango S; Centre Pasteur du Cameroun, Yaoundé, Cameroon., Tume CB; Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, West Region, Cameroon., Parr JB; Institute for Global Health and Infectious Diseases, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America.; Curriculum in Genetics and Molecular Biology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America.; Division of Infectious Diseases, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America., Ali IM; Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, West Region, Cameroon., Bailey JA; Department of Pathology and Laboratory Medicine, Warren Alpert Medical School, Brown University, Providence, Rhode Island.; Data Science Institute, Center for Computational and Molecular Biology, Brown University, Providence, Rhode Island., Juliano JJ; Institute for Global Health and Infectious Diseases, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America.; Curriculum in Genetics and Molecular Biology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America.; Division of Infectious Diseases, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America.; Department of Epidemiology, Gillings School of Global Public Health, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America.

Publikováno v: MedRxiv : the preprint server for health sciences [medRxiv] 2024 Oct 10. Date of Electronic Publication: 2024 Oct 10.

Detection of P. malariae using a new rapid isothermal amplification lateral flow assay.

Autor: Assefa A; Ethiopian Public Health Institute, Addis Ababa, Ethiopia. ashyaega@yahoo.com.; Institute of Global Health and Infectious Diseases, University of North Carolina School of Medicine, Chapel Hill, NC, USA. ashyaega@yahoo.com., Wamae K; Institute of Global Health and Infectious Diseases, University of North Carolina School of Medicine, Chapel Hill, NC, USA., Hennelly CM; Institute of Global Health and Infectious Diseases, University of North Carolina School of Medicine, Chapel Hill, NC, USA., Ngasala B; Department of Parasitology and Medical Entomology, Muhimbili University of Health and Allied Sciences, Dar es Salaam, Tanzania., Muller M; Institute of Global Health and Infectious Diseases, University of North Carolina School of Medicine, Chapel Hill, NC, USA., Kalonji A; SANRU Asbl, Kinshasa, Democratic Republic of the Congo., Phanzu F; SANRU Asbl, Kinshasa, Democratic Republic of the Congo., Cunningham CH; Institute of Global Health and Infectious Diseases, University of North Carolina School of Medicine, Chapel Hill, NC, USA., Lin JT; Institute of Global Health and Infectious Diseases, University of North Carolina School of Medicine, Chapel Hill, NC, USA., Parr JB; Institute of Global Health and Infectious Diseases, University of North Carolina School of Medicine, Chapel Hill, NC, USA. jonathan_parr@med.unc.edu.

Publikováno v: Malaria journal [Malar J] 2024 Apr 12; Vol. 23 (1), pp. 104. Date of Electronic Publication: 2024 Apr 12.

Amplicon Sequencing as a Potential Surveillance Tool for Complexity of Infection and Drug Resistance Markers in Plasmodium falciparum Asymptomatic Infections.

Autor: Wamae K; Kenya Medical Research Institute (KEMRI)-Wellcome Trust Research Programme, Kilifi, Kenya., Kimenyi KM; Kenya Medical Research Institute (KEMRI)-Wellcome Trust Research Programme, Kilifi, Kenya.; Centre for Biotechnology and Bioinformatics, University of Nairobi, Nairobi, Kenya., Osoti V; Kenya Medical Research Institute (KEMRI)-Wellcome Trust Research Programme, Kilifi, Kenya., de Laurent ZR; Kenya Medical Research Institute (KEMRI)-Wellcome Trust Research Programme, Kilifi, Kenya., Ndwiga L; Kenya Medical Research Institute (KEMRI)-Wellcome Trust Research Programme, Kilifi, Kenya., Kharabora O; Division of Infectious Diseases, Department of Medicine, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA., Hathaway NJ; Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, USA., Bailey JA; Department of Pathology and Laboratory Medicine, Warren Alpert Medical School, Brown University, Providence, Rhode Island, USA., Juliano JJ; Division of Infectious Diseases, Department of Medicine, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.; Department of Epidemiology, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, North Carolina, USA.; Curriculum in Genetics and Molecular Biology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA., Bejon P; Kenya Medical Research Institute (KEMRI)-Wellcome Trust Research Programme, Kilifi, Kenya.; Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom., Ochola-Oyier LI; Kenya Medical Research Institute (KEMRI)-Wellcome Trust Research Programme, Kilifi, Kenya.

Publikováno v: The Journal of infectious diseases [J Infect Dis] 2022 Sep 13; Vol. 226 (5), pp. 920-927.

Targeted Amplicon Deep Sequencing for Monitoring Antimalarial Resistance Markers in Western Kenya.

Autor: Osoti V; KEMRI-Wellcome Trust Collaborative Programme, Kilifi, Kenya., Akinyi M; KEMRI-Wellcome Trust Collaborative Programme, Kilifi, Kenya.; Institute of Primate Research, National Museums of Kenya, Nairobi, Kenya., Wamae K; KEMRI-Wellcome Trust Collaborative Programme, Kilifi, Kenya., Kimenyi KM; KEMRI-Wellcome Trust Collaborative Programme, Kilifi, Kenya.; Department of Biochemistry, University of Nairobi, Nairobi, Kenya., de Laurent Z; KEMRI-Wellcome Trust Collaborative Programme, Kilifi, Kenya., Ndwiga L; KEMRI-Wellcome Trust Collaborative Programme, Kilifi, Kenya., Gichuki P; Eastern and Southern Africa Centre of International Parasite Control, Kenya Medical Research Institutegrid.33058.3d, Nairobi, Kenya., Okoyo C; Eastern and Southern Africa Centre of International Parasite Control, Kenya Medical Research Institutegrid.33058.3d, Nairobi, Kenya., Kepha S; Eastern and Southern Africa Centre of International Parasite Control, Kenya Medical Research Institutegrid.33058.3d, Nairobi, Kenya., Mwandawiro C; Eastern and Southern Africa Centre of International Parasite Control, Kenya Medical Research Institutegrid.33058.3d, Nairobi, Kenya., Kandie R; Division of National Malaria Programme, Ministry of Health, Nairobi, Kenya., Bejon P; KEMRI-Wellcome Trust Collaborative Programme, Kilifi, Kenya.; Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom., Snow RW; KEMRI-Wellcome Trust Collaborative Programme, Kilifi, Kenya.; Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom., Ochola-Oyier LI; KEMRI-Wellcome Trust Collaborative Programme, Kilifi, Kenya.

Publikováno v: Antimicrobial agents and chemotherapy [Antimicrob Agents Chemother] 2022 Apr 19; Vol. 66 (4), pp. e0194521. Date of Electronic Publication: 2022 Mar 10.