Zobrazeno 1 - 7
of 7
pro vyhledávání: '"Kevin J. Barringer"'
Autor:
Thomas R. Gingeras, Douglas D. Richman, Terrie Latimer, John C. Guatelli, Kevin J. Barringer, Patricia C. Prodanovich
Publikováno v:
Journal of Infectious Diseases. 164:1066-1074
Mutations at amino acid positions 67, 70, 215, and 219 in the human immunodeficiency virus type 1 (HIV-1) pol gene correlate with the emergence of resistance to zidovudine (AZT). These four positions were monitored in viral RNA extracted from infecte
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3fe1405aae77c28935e3fe506190663a
https://doi.org/10.1093/infdis/164.6.1066
https://doi.org/10.1093/infdis/164.6.1066
Publikováno v:
Gene. 89:117-122
A ligase-based, in vitro DNA amplification system (LAR) has been described by Wu and Wallace [Genomics 4 (1989) 560-569]. This strategy is based on the ability of a DNA ligase to join the 5' phosphate of one DNA molecule to the 3' hydroxyl of a secon
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c4f5870b65acf951d5c760e3baf6d743
https://doi.org/10.1016/0378-1119(90)90213-b
https://doi.org/10.1016/0378-1119(90)90213-b
Autor:
John C. Guatelli, Deborah Y. Kwoh, Kevin J. Barringer, Kristina Marie Whitfield, Thomas R. Gingeras, Douglas D. Richman
Publikováno v:
Scopus-Elsevier
A target nucleic acid sequence can be replicated (amplified) exponentially in vitro under isothermal conditions by using three enzymatic activities essential to retroviral replication: reverse transcriptase, RNase H, and a DNA-dependent RNA polymeras
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::25379fe1b0d3d3d8d08d070fdfea5a96
https://doi.org/10.1073/pnas.87.19.7797-a
https://doi.org/10.1073/pnas.87.19.7797-a
Publikováno v:
Molecular and Cellular Biology. 5:3376-3385
We developed a methylotrophic yeast, Pichia pastoris, as a host for DNA transformations. The system is based on an auxotrophic mutant host of P. pastoris which is defective in histidinol dehydrogenase. As a selectable marker, we isolated and characte
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::52f513320d6d6cfef979156bcb34e8cb
https://doi.org/10.1128/mcb.5.12.3376
https://doi.org/10.1128/mcb.5.12.3376
Autor:
Cathy A. Stillman, Kevin J. Barringer, Patricia J Koutz, James M. Cregg, Geneva Ruth Davis, Gregory Patrick Thill
Publikováno v:
Yeast. 5:167-177
In methylotrophic yeasts, alcohol oxidase is the first enzyme in the methanol-utilization pathway. The genome of one such yeast, Pichia pastoris, contains two alcohol oxidase genes, AOX1 and AOX2. Sequence analysis indicated that each gene encodes a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1daa2e9d1610f21f8457ba7e560bdddc
https://doi.org/10.1002/yea.320050306
https://doi.org/10.1002/yea.320050306
Publikováno v:
Molecular and Cellular Biology. 9:1316-1323
In Pichia pastoris, alcohol oxidase (AOX) is the first enzyme in the methanol utilization pathway and is encoded by two genes, AOX1 and AOX2. The DNA and predicted amino acid sequences of the protein-coding portions of the genes are closely homologou
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::26cfdb0bcca18d7aeafdc67332df6cd7
https://doi.org/10.1128/mcb.9.3.1316-1323.1989
https://doi.org/10.1128/mcb.9.3.1316-1323.1989
Publikováno v:
DNA (Mary Ann Liebert, Inc.). 6(2)
A chicken genomic library was screened with a cDNA probe containing the 3' coding and noncoding portions of quail fast-twitch skeletal muscle myosin heavy chain (MHC). This probe hybridized to seven to nine bands on Southern blots of chicken genomic
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::38f2bbb28522df46eac1c938737f3ae5
https://pubmed.ncbi.nlm.nih.gov/3034534
https://pubmed.ncbi.nlm.nih.gov/3034534