A pipeline for precise and efficient genome editing by sgRNA-Cas9 RNPs in Drosophila

Autor: Kevin G. Nyberg, Joseph Q. Nguyen, Yong-Jae Kwon, Shelby Blythe, Greg J. Beitel, Richard Carthew

Publikováno v: Fly, Vol 14, Iss 1-4, Pp 34-48 (2020)

Genome editing via homology-directed repair (HDR) has made possible precise and deliberate modifications to gene sequences. CRISPR/Cas9-mediated HDR is the simplest means to carry this out. However, technical challenges remain to improve efficiency a

Externí odkaz: https://doaj.org/article/8a34f8df85884985b1caa1ddcad3a880

CRISPR-/Cas9-Mediated Precise and Efficient Genome Editing in Drosophila

Autor: Kevin G. Nyberg, Richard W. Carthew

Publikováno v: Methods Mol Biol
Methods in Molecular Biology ISBN: 9781071625408

The CRISPR/Cas9 system provides the means to make precise and purposeful modifications to the genome via homology-directed repair (HDR). In Drosophila, a wide variety of tools provide flexibility to achieve these ends. Here, we detail a method to gen

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::290da187bc37d212961e87920c58fa2b
https://europepmc.org/articles/PMC10027364/

Comparative Expression Dynamics of Intergenic Long Noncoding RNAs in the GenusDrosophila

Autor: Carlos A. Machado, Kevin G. Nyberg

Publikováno v: Genome Biology and Evolution

Thousands of long noncoding RNAs (lncRNAs) have been annotated in eukaryotic genomes, but comparative transcriptomic approaches are necessary to understand their biological impact and evolution. To facilitate such comparative studies in Drosophila, w

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6313f3e8308b0d3922893b574ca6299e
https://doi.org/10.1093/gbe/evw116

Genomes of 13 domesticated and wild rice relatives highlight genetic conservation, turnover and innovation across the genus Oryza

Autor: Seunghee Lee, Jetty S.S. Ammiraju, Railson Schreinert dos Santos, Ann Danowitz, Shu-Min Kao, Li Zhang, Chengjun Zhang, Cheng chieh Wu, Dongying Gao, Carlos E.M. Londono, Scott A. Jackson, Yi Liao, Mingsheng Chen, Chuanzhu Fan, Andrea Zuccolo, Muhua Wang, Christos Noutsos, Rod A. Wing, Manyuan Long, Robert J Henry, Marie-Christine Carpentier, Kshirod K. Jena, Aiko Iwata, Yue-Ie C. Hsing, Jose Luis Goicoechea, Bin Han, Richard Cooke, Joshua C. Stein, Luis F. Rivera, Thomas Wicker, Dario Copetti, Fu Jin Wei, Claude Becker, Paul L. Sanchez, Qi Feng, Andrea R. Gschwend, Ramil Mauleon, Carlos A. Machado, Derrick J. Zwickl, Daniel da Rosa Farias, Jayson Talag, Dave Flowers, Eric Lasserre, Nickolai Alexandrov, Yeisoo Yu, Moaine El Baidouri, Luciano Carlos da Maia, Jeremy Schmutz, Dave Kudrna, Olivier Panaud, Kenneth L. McNally, Xiang Song, Kevin G. Nyberg, Nori Kurata, Qiang Zhao, Kapeel Chougule, Jhih wun Zeng, Antonio Costa de Oliveira, Jianwei Zhang, Doreen Ware, Jun Wang, Detlef Weigel, Sharon Wei, Julie Jacquemin, Michael J. Sanderson

Publikováno v: Nature genetics, vol 50, iss 2
Nature Genetics
Nature Genetics, Nature Publishing Group, 2018, 50 (2), pp.285-296. ⟨10.1038/s41588-018-0040-0⟩

The genus Oryza is a model system for the study of molecular evolution over time scales ranging from a few thousand to 15 million years. Using 13 reference genomes spanning the Oryza species tree, we show that despite few large-scale chromosomal rear

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4e092dbb9a19537bbd53d281805c116a
https://escholarship.org/uc/item/69s9j770

Self-masking in an Intact ERM-merlin Protein: An Active Role for the Central α-Helical Domain

Autor: Rima Kulikauskas, Richard G. Fehon, Mark R. Nance, Qianzhi Li, John J.G. Tesmer, P. Andrew Karplus, Kevin G. Nyberg, Anthony Bretscher

Publikováno v: Journal of Molecular Biology. 365:1446-1459

Ezrin/radixin/moesin (ERM) family members provide a regulated link between the cortical actin cytoskeleton and the plasma membrane to govern membrane structure and organization. Here, we report the crystal structure of intact insect moesin, revealing

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b7fee2fe3b25d109545c83de1f0bcada
https://doi.org/10.1016/j.jmb.2006.10.075