Výsledky vyhledávání - "Kevin Entzminger"

Akademický článek

Western blotting of native proteins from agarose gels

Autor: Chiaki Sakuma, Masataka Nakagawa, Yui Tomioka, Toshiaki Maruyama, Kevin Entzminger, Jonathan K Fleming, Takashi Shibata, Yasunori Kurosawa, CJ Okumura, Tsutomu Arakawa, Teruo Akuta

Publikováno v: BioTechniques, Vol 72, Iss 5, Pp 207-218 (2022)

We have developed a new Western blotting method of native proteins from agarose-based gel electrophoresis using a buffer at pH 6.1 containing basic histidine and acidic 2-(N-morpholino)ethanesulfonic acid. This gel electrophoresis successfully provid

Externí odkaz: https://doaj.org/article/617c78fb8b3e44e2b265eac57674e82c

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Akademický článek

A New Method to Characterize Conformation-Specific Antibody by a Combination of Agarose Native Gel Electrophoresis and Contact Blotting

Autor: Teruo Akuta, Toshiaki Maruyama, Chiaki Sakuma, Masataka Nakagawa, Yui Tomioka, Kevin Entzminger, Jonathan K. Fleming, Ryo Sato, Takashi Shibata, Yasunori Kurosawa, C. J. Okumura, Tsutomu Arakawa

Publikováno v: Antibodies, Vol 11, Iss 2, p 36 (2022)

In this study, we review the agarose native gel electrophoresis that separates proteins and macromolecular complexes in their native state and transfer of the separated proteins from the agarose gel to membranes by contact blotting which retains the

Externí odkaz: https://doaj.org/article/906228a9cdd34f64873d8605805429af

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Antibody recognition of complement factor H reveals a flexible loop involved in atypical hemolytic uremic syndrome pathogenesis

Autor: Takanori Yokoo, Aki Tanabe, Yoko Yoshida, Jose M.M. Caaveiro, Makoto Nakakido, Yoichiro Ikeda, Yoshihiro Fujimura, Masaneori Matsumoto, Kevin Entzminger, Toshiaki Maruyama, C.J. Okumura, Masaomi Nangaku, Kouhei Tsumoto

Publikováno v: J Biol Chem

Atypical hemolytic uremic syndrome (aHUS) is a disease associated with dysregulation of the immune complement system, especially of the alternative pathway (AP). Complement factor H (CFH), consisting of 20 domains called complement control protein (C

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::06490d18e155ce302f1960c31539cef9
https://pubmed.ncbi.nlm.nih.gov/35868160

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Correction: Antibody recognition of complement factor H reveals a flexible loop involved in atypical hemolytic uremic syndrome pathogenesis

Publikováno v: Journal of Biological Chemistry. 298:102262

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::583273d32b7c6c6e9839afced67e171f
https://doi.org/10.1016/j.jbc.2022.102262

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