Zobrazeno 1 - 10
of 21
pro vyhledávání: '"Kevin D. Whitley"'
Autor:
Stuart Middlemiss, Matthieu Blandenet, David M. Roberts, Andrew McMahon, James Grimshaw, Joshua M. Edwards, Zikai Sun, Kevin D. Whitley, Thierry Blu, Henrik Strahl, Séamus Holden
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-13 (2024)
Abstract Most rod-shaped bacteria elongate by inserting new cell wall material into the inner surface of the cell sidewall. This is performed by class A penicillin binding proteins (PBPs) and a highly conserved protein complex, the elongasome, which
Externí odkaz:
https://doaj.org/article/aa29a5c2d20c4c05b440ebadd6070e85
Autor:
Sean P. Carney, Wen Ma, Kevin D. Whitley, Haifeng Jia, Timothy M. Lohman, Zaida Luthey-Schulten, Yann R. Chemla
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-14 (2021)
UvrD is a model helicase from the non-hexameric Superfamily 1. Here, the authors use optical tweezers to measure directly the stepwise translocation of UvrD along a DNA hairpin, and propose a mechanism in which UvrD moves one base pair at a time, but
Externí odkaz:
https://doaj.org/article/787da31e7c364e8b9f5fc0478dc6983b
Autor:
Kevin D. Whitley, Calum Jukes, Nicholas Tregidgo, Eleni Karinou, Pedro Almada, Yann Cesbron, Ricardo Henriques, Cees Dekker, Séamus Holden
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-13 (2021)
Bacterial cell division by cell wall synthesis proteins is guided by treadmilling filaments of the cytoskeleton protein FtsZ. Here authors use nanofabrication, advanced microscopy, and microfluidics to resolve the function of FtsZ treadmilling in the
Externí odkaz:
https://doaj.org/article/f128d22694da419c86e73cfe7331df52
Autor:
Stuart Middlemiss, David M Roberts, James Grimshaw, Joshua M Edwards, Zikai Sun, Kevin D Whitley, Thierry Blu, Henrik Strahl, Séamus Holden
Most rod-shaped bacteria elongate by inserting new cell wall material into the inner surface of the cell sidewall. This is primarily performed by a highly conserved protein complex, the elongasome, which moves processively around the cell circumferen
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e15087c3060956780c89888d3f6fd8e1
https://doi.org/10.1101/2023.05.10.540107
https://doi.org/10.1101/2023.05.10.540107
Autor:
Christian Vanhille-Campos, Kevin D. Whitley, Philipp Radler, Martin Loose, Séamus Holden, Anđela Šarić
Functional protein filaments in the cell commonly treadmill – grow and shrink on opposite ends, driven by energy consumption. Treadmilling causes filaments to seem as if they were moving, even though the individual proteins remain static. Here we i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b706ef7b3a60394c108bf48c4095b06a
https://doi.org/10.1101/2023.05.08.539808
https://doi.org/10.1101/2023.05.08.539808
Autor:
Haifeng Jia, Yann R. Chemla, Sean P. Carney, Zaida Luthey-Schulten, Timothy M. Lohman, Wen Ma, Kevin D. Whitley
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-14 (2021)
Nature Communications
Nature Communications
UvrD, a model for non-hexameric Superfamily 1 helicases, utilizes ATP hydrolysis to translocate stepwise along single-stranded DNA and unwind the duplex. Previous estimates of its step size have been indirect, and a consensus on its stepping mechanis
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a844ecdf4de680681b93d0ffd6d5c399
https://doaj.org/article/787da31e7c364e8b9f5fc0478dc6983b
https://doaj.org/article/787da31e7c364e8b9f5fc0478dc6983b
Publikováno v:
eLife, Vol 7 (2018)
Helicases play key roles in genome maintenance, yet it remains elusive how these enzymes change conformations and how transitions between different conformational states regulate nucleic acid reshaping. Here, we developed a computational technique co
Externí odkaz:
https://doaj.org/article/49a704fdf85a45ba83431a121495e928
Publikováno v:
Nucleic Acids Research
Helicases are biomolecular motors that unwind nucleic acids, and their regulation is essential for proper maintenance of genomic integrity. Escherichia coli Rep helicase, whose primary role is to help restart stalled replication, serves as a model fo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d40504aa3c10c00430850e4038bc897f
https://doi.org/10.1093/nar/gkz023
https://doi.org/10.1093/nar/gkz023
Autor:
Ricardo Henriques, Pedro Almada, Cees Dekker, Nicholas Tregidgo, Eleni Karinou, Seamus Holden, Yann Cesbron, Calum Jukes, Kevin D. Whitley
Publikováno v:
Nature Communications, 12(1)
Nature Communications
Nature Communications, Vol 12, Iss 1, Pp 1-13 (2021)
Nature Communications
Nature Communications, Vol 12, Iss 1, Pp 1-13 (2021)
Despite the central role of division in bacterial physiology, how division proteins work together as a nanoscale machine to divide the cell remains poorly understood. Cell division by cell wall synthesis proteins is guided by the cytoskeleton protein
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::62f643c307650e1a93416cb809df18c0
http://resolver.tudelft.nl/uuid:718a558c-149f-433d-a482-c3cf25fad032
http://resolver.tudelft.nl/uuid:718a558c-149f-433d-a482-c3cf25fad032
Autor:
Eleni Karinou, Seamus Holden, Calum Jukes, Ricardo Henriques, Pedro Almada, Cees Dekker, Nicholas Tregidgo, Kevin D. Whitley
Despite the central role of division in bacterial physiology, how division proteins work together as a nanoscale machine to divide the cell remains poorly understood. Cell division by cell wall synthesis proteins is guided by the cytoskeleton protein
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::560ca2e502d7c3af96efe4a8a46a76d7
https://doi.org/10.1101/2020.07.01.182006
https://doi.org/10.1101/2020.07.01.182006