ReScan, a Multiplex Diagnostic Pipeline, Pans Human Sera for SARS-CoV-2 Antigens

Autor: Colin R. Zamecnik, Jayant V. Rajan, Kevin A. Yamauchi, Sabrina A. Mann, Rita P. Loudermilk, Gavin M. Sowa, Kelsey C. Zorn, Bonny D. Alvarenga, Christian Gaebler, Marina Caskey, Mars Stone, Philip J. Norris, Wei Gu, Charles Y. Chiu, Dianna Ng, James R. Byrnes, Xin X. Zhou, James A. Wells, Davide F. Robbiani, Michel C. Nussenzweig, Joseph L. DeRisi, Michael R. Wilson

Publikováno v: Cell Reports Medicine, Vol 1, Iss 7, Pp 100123- (2020)

Summary: Comprehensive understanding of the serological response to SARS-CoV-2 infection is important for both pathophysiologic insight and diagnostic development. Here, we generate a pan-human coronavirus programmable phage display assay to perform

Externí odkaz: https://doaj.org/article/fb798e3575304cdbbba3b382901cca64

SpatialData: an open and universal data framework for spatial omics

Autor: Luca Marconato, Giovanni Palla, Kevin A. Yamauchi, Isaac Virshup, Elyas Heidari, Tim Treis, Marcella Toth, Rahul B. Shrestha, Harald Vöhringer, Wolfgang Huber, Moritz Gerstung, Josh Moore, Fabian J. Theis, Oliver Stegle

Spatially resolved omics technologies are transforming our understanding of biological tissues. However, handling uni- and multi-modal spatial omics datasets remains a challenge owing to large volumes of data, heterogeneous data types and the lack of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::5b07735a131967c80c7064bb9abb418a
https://doi.org/10.1101/2023.05.05.539647

Validation and tuning of in situ transcriptomics image processing workflows with crowdsourced annotations

Autor: Rafael Gomez-Sjoberg, Jenny M. Vo-Phamhi, Kevin A. Yamauchi

Publikováno v: PLoS Computational Biology, Vol 17, Iss 8, p e1009274 (2021)
PLoS Computational Biology

Recent advancements in in situ methods, such as multiplexed in situ RNA hybridization and in situ RNA sequencing, have deepened our understanding of the way biological processes are spatially organized in tissues. Automated image processing and spot-

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::10f681771c6678da3994a4ee07635a72
https://doaj.org/article/36be295d2ebd43fea6174c1e404119e8

Laterally Aggregated Polyacrylamide Gels for Immunoprobed Isoelectric Focusing

Autor: Shaheen Jeeawoody, Alison Su, Kevin A. Yamauchi, Amy E. Herr

Publikováno v: Anal Chem

Immunoprobed isoelectric focusing (IEF) resolves proteins based on differences in isoelectric point (pI) and then identifies protein targets through immunoprobing of IEF-separated proteins that have been immobilized onto a gel scaffold. During the IE

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7dd24687fa6309cb124e782a1f5849bc
https://europepmc.org/articles/PMC7861876/

A single-cell transcriptomic atlas characterizes ageing tissues in the mouse

Autor: Nicholas Schaum, Ashley Maynard, Kenneth I. Weinberg, Ishita Bansal, Annie Lo, Christin S. Kuo, Hamid Ebadi, Norma Neff, Bryan D. Merrill, Gunsagar S. Gulati, Michelle B. Chen, Nathalie Khoury, Song E. Lee, Martin J. Zhang, Michael N. Wosczyna, Linda J. van Weele, Lakshmi P. Yerra, James Zou, Matt Fish, Michael F. Clarke, Antoine de Morrée, Lucas M. Waldburger, Kyle J. Travaglini, Maria F. Lugo-Fagundo, Yan Hang, Rasika Patkar, Kerwyn Casey Huang, Weng Chuan Peng, Wan Jin Lu, Astrid Gillich, Andrew May, Aaron Demers, Tony Wyss-Coray, Benoit Lehallier, William Kong, Douglas Brownfield, Robert C. Jones, Katharine M. Ng, Ankit S. Baghel, Patricia K. Nguyen, Rafael Gòmez-Sjöberg, Katherine S. Pollard, Ling Liu, Kevin S. Kao, Róbert Pálovics, Taichi Isobe, Chunyu Zhao, Roel Nusse, Eric J. Rulifson, Maya E. Kumar, Bruce Wang, Philip A. Beachy, Tessa Divita, Ross J. Metzger, Cristina M. Tato, Thomas A. Rando, Marina McKay, Hui Zhang, Oliver Hahn, Jinyi Xiang, Jane Antony, Aaron McGeever, Daniel Staehli, Macy E. Zardeneta, Tal Iram, Olivia Leventhal, Qingyun Li, Angela Oliveira Pisco, Lolita Penland, Krissie Tellez, Marco Mignardi, Brian Yu, Shaheen S. Sikandar, Lincoln Harris, Nicole Almanzar, Corey Cain, Geraldine Genetiano, Foad Green, Davis P. Lee, Carolina Tropini, Laughing Bear Torrez Dulgeroff, Rahul Sinha, Zhen Qi, Stephen R. Quake, Charles Chan, Irving L. Weissman, Sean M. Wu, Jim Karkanias, Ahmad N. Nabhan, Andreas Keller, Margaret Tsui, Alexander Zee, Guruswamy Karnam, Michael S. Haney, Haley du Bois, Robert Puccinelli, Ben A. Barres, Justin L. Sonnenburg, F. Hernan Espinoza, Fabio Zanini, Qiang Gan, Joseph Noh, Lu Zhou, Isaac Bakerman, Aaron M. Kershner, Mark A. Krasnow, Kubilay Demir, Feather Ives, Benson M. George, Guang Li, Dullei Min, Justin Youngyunpipatkul, Mu He, Rene V. Sit, Bernhard M. Kiss, Stephanie D. Conley, Seung K. Kim, Weilun Tan, Soso Xue, M. Windy McNerney, Andrew C. Yang, Kevin A. Yamauchi, Albin Huang, Joe M. Segal, Krzysztof Szade, Michelle Tan, Biter Bilen, Fan Zhang, Spyros Darmanis, Shayan Hosseinzadeh, Xueying Gu, Jonathan K. Lam, Anoop Manjunath, Daniela Berdnik

Publikováno v: Nature

Aging is characterized by a progressive loss of physiological integrity, leading to impaired function and increased vulnerability to death(1). Despite rapid advances over recent years, many of the molecular and cellular processes which underlie progr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a1c3489ceeac8cf1e8dc138771ff6428
https://ruj.uj.edu.pl/xmlui/handle/item/279563