Zobrazeno 1 - 10
of 44
pro vyhledávání: '"Keunsub Lee"'
Publikováno v:
Frontiers in Plant Science, Vol 15 (2024)
The RUBY reporter system has demonstrated great potential as a visible marker to monitor gene expression in both transiently and stably transformed plant tissues. Ectopic expression of the RUBY reporter leads to bright red pigmentation in plant tissu
Externí odkaz:
https://doaj.org/article/34dbefb7ad3d4d0c924de49b3df397ee
Publikováno v:
Frontiers in Plant Science, Vol 15 (2024)
Agrobacterium-mediated transformation is an essential tool for functional genomics studies and crop improvements. Recently developed ternary vector systems, which consist of a T-DNA vector and a compatible virulence (vir) gene helper plasmid (ternary
Externí odkaz:
https://doaj.org/article/536ab78d6ca9433f95ba5baeb04996fa
Publikováno v:
Plants, Vol 12, Iss 15, p 2799 (2023)
Efficient genetic transformation is a prerequisite for rapid gene functional analyses and crop trait improvements. We recently demonstrated that new T-DNA binary vectors with NptII/G418 selection and a compatible helper plasmid can efficiently transf
Externí odkaz:
https://doaj.org/article/2001cd68167c4ae690457aa890a132e9
Autor:
Kyle Miller, Alan L. Eggenberger, Keunsub Lee, Fei Liu, Minjeong Kang, Madison Drent, Andrew Ruba, Tyler Kirscht, Kan Wang, Shan Jiang
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
Abstract Biolistic delivery is widely used for genetic transformation but inconsistency between bombardment samples for transient gene expression analysis often hinders quantitative analyses. We developed a methodology to improve the consistency of b
Externí odkaz:
https://doaj.org/article/cf7ad386493a4ad5925bfe5e9dd79613
Publikováno v:
Frontiers in Plant Science, Vol 13 (2022)
For maize genome-editing and bioengineering, genetic transformation of inbred genotypes is most desired due to the uniformity of genetic background in their progenies. However, most maize inbred lines are recalcitrant to tissue culture and transforma
Externí odkaz:
https://doaj.org/article/fd234679e97e40dc9e64b437e28b534e
Publikováno v:
Frontiers in Plant Science, Vol 12 (2021)
Modern maize exhibits a significantly different phenotype than its wild progenitor teosinte despite many genetic similarities. Of the many subspecies of Zea mays identified as teosinte, Zea mays ssp. parviglumis is the most closely related to domesti
Externí odkaz:
https://doaj.org/article/7cb444e4cef94dab865e6847eb07ced5
Autor:
Aimee A. Malzahn, Xu Tang, Keunsub Lee, Qiurong Ren, Simon Sretenovic, Yingxiao Zhang, Hongqiao Chen, Minjeong Kang, Yu Bao, Xuelian Zheng, Kejun Deng, Tao Zhang, Valeria Salcedo, Kan Wang, Yong Zhang, Yiping Qi
Publikováno v:
BMC Biology, Vol 17, Iss 1, Pp 1-14 (2019)
Abstract Background CRISPR-Cas12a (formerly Cpf1) is an RNA-guided endonuclease with distinct features that have expanded genome editing capabilities. Cas12a-mediated genome editing is temperature sensitive in plants, but a lack of a comprehensive un
Externí odkaz:
https://doaj.org/article/90a2e580ce324569b3ff72d77e2eb6cb
Autor:
Morgan E. McCaw, Keunsub Lee, Minjeong Kang, Jacob D. Zobrist, Mercy K. Azanu, James A. Birchler, Kan Wang
Publikováno v:
Frontiers in Genome Editing, Vol 2 (2021)
Maize (Zea mays ssp. mays) is a popular genetic model due to its ease of crossing, well-established toolkits, and its status as a major global food crop. Recent technology developments for precise manipulation of the genome are further impacting both
Externí odkaz:
https://doaj.org/article/840c6b0bf9984aea894dbd8e33895fbd
Autor:
Keunsub Lee, Xiaoqiu Huang, Chichun Yang, Danny Lee, Vincent Ho, Kan Nobuta, Jian-Bing Fan, Kan Wang
Publikováno v:
PLoS ONE, Vol 8, Iss 8, p e70720 (2013)
Agrobacterium tumefaciens is a plant pathogen that has the natural ability of delivering and integrating a piece of its own DNA into plant genome. Although bacterial non-coding RNAs (ncRNAs) have been shown to regulate various biological processes in
Externí odkaz:
https://doaj.org/article/495908e1454a46bdbe096304de6e7587
Publikováno v:
Plant Biotechnology Journal. 20:1916-1927
Agrobacterium tumefaciens, the causal agent of plant crown gall disease, has been widely used to genetically transform many plant species. The inter-kingdom gene transfer capability made Agrobacterium an essential tool and model system to study the m
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::137e3ce42816c3e2978832b0f11b49ba
https://doi.org/10.1111/pbi.13872
https://doi.org/10.1111/pbi.13872