Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Kerstin Crusius"'
Autor:
S. Miranda, Peter Donner, M. A. A. Thomaz, Roman Hillig, Vera Pütter, Caterina Holz, Jörg Fanghänel, Uwe Eberspaecher, Maria Arménia Carrondo, Pedro M. Matias, Patrick Amstutz, Christine Lang, Kerstin Crusius, Maya Gulotti-Georgieva, Beate Muller-Tiemann, H. Kaspar Binz, Ursula Egner, Tiago M. Bandeiras, Arndt Schmitz
Publikováno v:
Acta Crystallographica Section D Biological Crystallography. 64:339-353
As a key regulator of mitosis, the Ser/Thr protein polo-like kinase-1 (Plk-1) is a well validated drug target in cancer therapy. In order to enable structure-guided drug design, determination of the crystal structure of the kinase domain of Plk-1 was
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::13f8b5b6e2a5898f1e802d4480ee6e5b
https://doi.org/10.1107/s0907444907068217
https://doi.org/10.1107/s0907444907068217
Autor:
Kerstin Crusius, Silke Finster, John McClary, Jian-Ai Xuan, Hong-Tao Lu, Alicia Newton, Brent Larsen, Peter Bringmann, Debbie Allen, Wei Xia, Ronald R. Cobb, Sara Biancalana, Douglas W. Schneider
Publikováno v:
Gene. 380:111-119
The detection and purification of proteins are often time-consuming and frequently involve complicated protocols. The addition of a peptide tag to recombinant proteins can make this process more efficient. Many of the commonly used tags, such as Flag
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::279aa55bcd45d25d28939b27a5475215
https://doi.org/10.1016/j.gene.2006.05.027
https://doi.org/10.1016/j.gene.2006.05.027
Autor:
Peter Donner, Maria Arménia Carrondo, Kerstin Crusius, M. A. A. Thomaz, Ricardo Coelho, Anja Wegg, Ursula Egner, Pedro M. Matias, Xiao-Yan Zhao
Publikováno v:
Journal of Medicinal Chemistry. 45:1439-1446
The crystal structure of a mutant androgen receptor (AR) ligand-binding domain (LBD) in complex with the agonist 9alpha-fluorocortisol has been determined at 1.95 A resolution. This mutant AR contains two mutations (L701H and T877A) and was previousl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d4fc9098ec5a91705d2e4dc9f1f588d0
https://doi.org/10.1021/jm011072j
https://doi.org/10.1021/jm011072j
Publikováno v:
Virus Genes. 20:65-69
The human papillomavirus type 16 E5 gene product has been shown to upregulate the activation of MAP kinases ERK1/2 and cellular proliferation promoted by EGF in a ligand-dependent process. We now report the growth factor-independent modulation of MAP
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2466ae129ce027270b6da2af2954e853
https://doi.org/10.1023/a:1008112207824
https://doi.org/10.1023/a:1008112207824
Publikováno v:
Oncogene. 18:6714-6718
The human papillomavirus type 16 E5 (HPV16-E5) protein is a membrane protein that has been associated with malignant growth. The protein affects growth factor-mediated signal transduction in a ligand-dependent manner. We show now that E5 expression i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::73e50d4c8c8b92a2651ef623f6ca75ca
https://doi.org/10.1038/sj.onc.1203075
https://doi.org/10.1038/sj.onc.1203075
Autor:
Guido A. Malawski, Arndt A.P. Schmitz, Martina Huber, Beate Müller-Tiemann, Kerstin Crusius, Roman C. Hillig, Ursula Egner, Uwe Eberspaecher, Felipe Monteclaro, Peter Donner
Publikováno v:
Protein science : a publication of the Protein Society. 15(12)
This study describes an efficient multiparallel automated workflow of cloning, expression, purification, and crystallization of a large set of construct variants for isolated protein domains aimed at structure determination by X-ray crystallography.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::64c3e016626880f16d311375127812f9
https://pubmed.ncbi.nlm.nih.gov/17132859
https://pubmed.ncbi.nlm.nih.gov/17132859
Publikováno v:
Experimental cell research. 241(1)
The E5 open reading frame of the human papillomavirus type 16 encodes a transmembrane protein associated with the Golgi, ER, and plasma membranes. We have analyzed the effect of E5 expression on the activation of the EGF receptor family. We find that
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e8e9e36b802e5c1034667588d0784d00
https://pubmed.ncbi.nlm.nih.gov/9633515
https://pubmed.ncbi.nlm.nih.gov/9633515
Publikováno v:
Oncogene. 15(12)
In this report we demonstrate that cells expressing the human papillomavirus type 16 E5 open reading frame (HPV16-E5) show a greatly enhanced transcription of the immediate early genes after EGF or PMA treatment compared to control cells. This enhanc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c863efb61f7b8e46aa9e9fc4aa41a2d0
https://pubmed.ncbi.nlm.nih.gov/9333019
https://pubmed.ncbi.nlm.nih.gov/9333019