Zobrazeno 1 - 10
of 40
pro vyhledávání: '"Kepin Wang"'
Autor:
Weikai Ge, Shixue Gou, Xiaozhu Zhao, Qin Jin, Zhenpeng Zhuang, Yu Zhao, Yanhui Liang, Zhen Ouyang, Xiaoyi Liu, Fangbing Chen, Hui Shi, Haizhao Yan, Han Wu, Liangxue Lai, Kepin Wang
Publikováno v:
Signal Transduction and Targeted Therapy, Vol 9, Iss 1, Pp 1-14 (2024)
Abstract The CRISPR/Cas9 system has shown great potential for treating human genetic diseases through gene therapy. However, there are concerns about the safety of this system, specifically related to the use of guide-free Cas9. Previous studies have
Externí odkaz:
https://doaj.org/article/d5821f95cdce4ed0a2e9fbac9fb1c3f0
Autor:
Hui Shi, Lei Li, Shuangshuang Mu, Shixue Gou, Xiaoyi Liu, Fangbing Chen, Menglong Chen, Qin Jin, Liangxue Lai, Kepin Wang
Publikováno v:
BMC Biology, Vol 22, Iss 1, Pp 1-16 (2024)
Abstract Background Many efforts have been made to improve the precision of Cas9-mediated gene editing through increasing knock-in efficiency and decreasing byproducts, which proved to be challenging. Results Here, we have developed a human exonuclea
Externí odkaz:
https://doaj.org/article/63f8d4db211341e188077f960aa406ff
Autor:
Shuangshuang Mu, Huangyao Chen, Qianru Li, Shixue Gou, Xiaoyi Liu, Junwei Wang, Wei Zheng, Menglong Chen, Qin Jin, Liangxue Lai, Kepin Wang, Hui Shi
Publikováno v:
Genome Biology, Vol 25, Iss 1, Pp 1-19 (2024)
Abstract Background Prime editing enables precise base substitutions, insertions, and deletions at targeted sites without the involvement of double-strand DNA breaks or exogenous donor DNA templates. However, the large size of prime editors (PEs) ham
Externí odkaz:
https://doaj.org/article/5d0ea2f60dda49b2aa237e960f70f26e
Publikováno v:
Animal Research and One Health, Vol 1, Iss 2, Pp 242-258 (2023)
Abstract Conditional gene regulation systems can control gene expression in predefined tissues or organs at a desired time. Site‐specific recombinase systems and chemically induced gene expression systems are the two most widely used approaches for
Externí odkaz:
https://doaj.org/article/a5c0fb3a277e441d985f364e2995080e
Autor:
Weikai Ge, Xiaozhu Zhao, Shixue Gou, Qin Jin, Fangbing Chen, Zhen Ouyang, Chengdan Lai, Tao Cui, Baoyi Mai, Sijia Lu, Kexin Zhong, Yanhui Liang, Tao Chen, Han Wu, Nan Li, Yinghua Ye, Liangxue Lai, Kepin Wang
Publikováno v:
Molecular Therapy: Nucleic Acids, Vol 34, Iss , Pp 102035- (2023)
Cas9 protein without sgRNAs can induce genomic damage at the cellular level in vitro. However, whether the detrimental effects occur in embryos after Cas9 treatment remains unknown. Here, using pig embryos as subjects, we observed that Cas9 protein t
Externí odkaz:
https://doaj.org/article/bbe5b6b43aba40698fb60acc055ce682
Autor:
Qin Jin, Xiaoyi Liu, Zhenpeng Zhuang, Jiayuan Huang, Shixue Gou, Hui Shi, Yu Zhao, Zhen Ouyang, Zhaoming Liu, Lei Li, Junjie Mao, Weikai Ge, Fangbing Chen, Manya Yu, Yezhi Guan, Yinghua Ye, Chengcheng Tang, Ren Huang, Kepin Wang, Liangxue Lai
Publikováno v:
Genome Biology, Vol 24, Iss 1, Pp 1-25 (2023)
Abstract Background CRISPR-based toolkits have dramatically increased the ease of genome and epigenome editing. SpCas9 is the most widely used nuclease. However, the difficulty of delivering SpCas9 and inability to modulate its expression in vivo hin
Externí odkaz:
https://doaj.org/article/110960d35b9b427bbaadfb937e744aa5
Autor:
Fangbing Chen, Meng Lian, Bingxiu Ma, Shixue Gou, Xian Luo, Kaiming Yang, Hui Shi, Jingke Xie, Weika Ge, Zhen Ouyang, Chengdan Lai, Nan Li, Quanjun Zhang, Qin Jin, Yanhui Liang, Tao Chen, Jiaowei Wang, Xiaozhu Zhao, Lei Li, Manya Yu, Yinghua Ye, Kepin Wang, Han Wu, Liangxue Lai
Publikováno v:
Communications Biology, Vol 5, Iss 1, Pp 1-12 (2022)
Cas12a-mediated cytosine (CBE) and adenine base editor (ABE) systems with elevated efficiencies and expanded target scopes are developed, by combining highly active deaminases with Cas12a variants, enCas12a, RR and RVR, recognizing non-TTTV PAMs.
Externí odkaz:
https://doaj.org/article/a070b51af0f64766bd9a718411c97efb
Autor:
Jingke Xie, Xingyun Huang, Xia Wang, Shixue Gou, Yanhui Liang, Fangbing Chen, Nan Li, Zhen Ouyang, Quanjun Zhang, Weikai Ge, Qin Jin, Hui Shi, Zhenpeng Zhuang, Xiaozhu Zhao, Meng Lian, Jiaowei Wang, Yinghua Ye, Longquan Quan, Han Wu, Kepin Wang, Liangxue Lai
Publikováno v:
BMC Biology, Vol 18, Iss 1, Pp 1-14 (2020)
Abstract Background Many favorable traits of crops and livestock and human genetic diseases arise from multiple single nucleotide polymorphisms or multiple point mutations with heterogeneous base substitutions at the same locus. Current cytosine or a
Externí odkaz:
https://doaj.org/article/5974bfd9c0c64e52a24cfd1869de793b
Autor:
Jingke Xie, Weikai Ge, Nan Li, Qishuai Liu, Fangbing Chen, Xiaoyu Yang, Xingyun Huang, Zhen Ouyang, Quanjun Zhang, Yu Zhao, Zhaoming Liu, Shixue Gou, Han Wu, Chengdan Lai, Nana Fan, Qin Jin, Hui Shi, Yanhui Liang, Ting Lan, Longquan Quan, Xiaoping Li, Kepin Wang, Liangxue Lai
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-13 (2019)
Base editors can efficiently produce single nucleotide alterations without requiring a double-strand break. Here the authors show base editing at multiple sites simultaneously in pigs.
Externí odkaz:
https://doaj.org/article/9abc3946a2d240f685ea0ef9c7de72aa
Autor:
Degong Ruan, Jiangyun Peng, Xiaoshan Wang, Zhen Ouyang, Qingjian Zou, Yi Yang, Fangbing Chen, Weikai Ge, Han Wu, Zhaoming Liu, Yu Zhao, Bentian Zhao, Quanjun Zhang, Chengdan Lai, Nana Fan, Zhiwei Zhou, Qishuai Liu, Nan Li, Qin Jin, Hui Shi, Jingke Xie, Hong Song, Xiaoyu Yang, Jiekai Chen, Kepin Wang, Xiaoping Li, Liangxue Lai
Publikováno v:
Stem Cell Reports, Vol 10, Iss 2, Pp 494-508 (2018)
Summary: Pig cloning by somatic cell nuclear transfer (SCNT) remains extremely inefficient, and many cloned embryos undergo abnormal development. Here, by profiling transcriptome expression, we observed dysregulated chromosome-wide gene expression in
Externí odkaz:
https://doaj.org/article/a4d9b29b70414a228e803c57bf09a08c