Zobrazeno 1 - 10
of 31
pro vyhledávání: '"Kent R. Thurber"'
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-14 (2020)
The low-complexity (LC) domain mediates liquid-liquid phase separation and fibril formation of the RNA-binding protein FUS (FUsed in Sarcoma). Here, the authors combine cryo-EM, solid-state NMR measurements and MD simulations to structurally characte
Externí odkaz:
https://doaj.org/article/8142805573524157b65818c9f357da0e
Publikováno v:
Journal of magnetic resonance (San Diego, Calif. : 1997). 342
We review recent efforts to develop and apply an experimental approach to the structural characterization of transient intermediate states in biomolecular processes that involve large changes in molecular conformation or assembly state. This approach
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::42f1468636abd1a115dc76c144e1fc37
https://pubmed.ncbi.nlm.nih.gov/35998398
https://pubmed.ncbi.nlm.nih.gov/35998398
Publikováno v:
J Struct Biol
Cryogenic electron microscopy (cryo-EM) is an important tool for determining the molecular structure of proteins and protein assemblies, including helical assemblies such as amyloid fibrils. In reconstruction of amyloid fibril structures from cryo-EM
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::32da241dbf01f104b361992370f34439
https://doi.org/10.1016/j.jsb.2021.107736
https://doi.org/10.1016/j.jsb.2021.107736
Publikováno v:
Proc Natl Acad Sci U S A
Amyloid-β (Aβ) fibrils exhibit self-propagating, molecular-level polymorphisms that may contribute to variations in clinical and pathological characteristics of Alzheimer's disease (AD). We report the molecular structure of a specific fibril polymo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0c712a8f59eeb510205f5b3acec805ac
https://doi.org/10.1073/pnas.2023089118
https://doi.org/10.1073/pnas.2023089118
Publikováno v:
Journal of Magnetic Resonance. 289:122-131
Solid-state dynamic nuclear polarization (DNP) using the cross-effect relies on radical pairs whose electron spin resonance (ESR) frequencies differ by the nuclear magnetic resonance (NMR) frequency. We measure the DNP provided by a new water-soluble
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f7fa11bc68e496a11c570d7771359eca
https://doi.org/10.1016/j.jmr.2018.02.016
https://doi.org/10.1016/j.jmr.2018.02.016
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America. 116(34)
Common experimental approaches for characterizing structural conversion processes such as protein folding and self-assembly do not report on all aspects of the evolution from an initial state to the final state. Here, we demonstrate an approach that
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::784ef4e36c79f869d5967ab80125f70a
https://pubmed.ncbi.nlm.nih.gov/31387974
https://pubmed.ncbi.nlm.nih.gov/31387974
Publikováno v:
Journal of the American Chemical Society. 137:8294-8307
Self-assembly of amyloid-β (Aβ) peptides in human brain tissue leads to neurodegeneration in Alzheimer's disease (AD). Amyloid fibrils, whose structures have been extensively characterized by solid state nuclear magnetic resonance (ssNMR) and other
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8dfcab483a82b0665521b455f8da0c77
https://doi.org/10.1021/jacs.5b04843
https://doi.org/10.1021/jacs.5b04843
Autor:
Yi Lin, Steven L. McKnight, Robert Tycko, Dylan T. Murray, Ivan Hung, Masato Kato, Kent R. Thurber
Publikováno v:
Cell, vol 171, iss 3
Polymerization and phase separation of proteins containing low-complexity (LC) domains are important factors in gene expression, mRNA processing and trafficking, and localization of translation. We have used solid-state nuclear magnetic resonance met
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::059fb843e8e2e4be72f45d9f8f995224
https://europepmc.org/articles/PMC5650524/
https://europepmc.org/articles/PMC5650524/
Autor:
Robert Tycko, Kent R. Thurber
Publikováno v:
Israel Journal of Chemistry. 54:39-46
Dynamic nuclear polarization (DNP) can dramatically increase the signal in nuclear magnetic resonance (NMR) experiments by transferring spin polarization from electrons to nuclei. We discuss quantum mechanical descriptions of the “solid effect” a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5ea2f84ff9ac65538f53479e860c87b9
https://doi.org/10.1002/ijch.201300116
https://doi.org/10.1002/ijch.201300116
Publikováno v:
Biophysical Journal. 116:193a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::aef7814df5f3f770255cda7abfe8196c
https://doi.org/10.1016/j.bpj.2018.11.1070
https://doi.org/10.1016/j.bpj.2018.11.1070