Structural basis of colibactin activation by the ClbP peptidase.

Autor: Velilla JA; Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA, USA., Volpe MR; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA., Kenney GE; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA., Walsh RM Jr; Harvard Cryo-EM Center for Structural Biology, Harvard Medical School, Boston, MA, USA.; Department of Biological Chemistry and Molecular Pharmacology, Blavatnik Institute, Harvard Medical School, Boston, MA, USA., Balskus EP; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA., Gaudet R; Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA, USA. gaudet@mcb.harvard.edu.

Publikováno v: Nature chemical biology [Nat Chem Biol] 2023 Feb; Vol. 19 (2), pp. 151-158. Date of Electronic Publication: 2022 Oct 17.

Revving an Engine of Human Metabolism: Activity Enhancement of Triosephosphate Isomerase via Hemi-Phosphorylation.

Autor: Schachner LF; Department of Chemistry, the Proteomics Center of Excellence, Northwestern University, Evanston, Illinois 60208, United States., Soye BD; Department of Chemistry, the Proteomics Center of Excellence, Northwestern University, Evanston, Illinois 60208, United States.; Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois 60208, United States., Ro S; Department Molecular and Biological Sciences, Northwestern University, Evanston, Illinois 60208, United States., Kenney GE; Department Molecular and Biological Sciences, Northwestern University, Evanston, Illinois 60208, United States.; Department of Chemistry, Harvard University, Cambridge, Massachusetts 02140, United States., Ives AN; Department of Chemistry, the Proteomics Center of Excellence, Northwestern University, Evanston, Illinois 60208, United States., Su T; Department of Chemistry, the Proteomics Center of Excellence, Northwestern University, Evanston, Illinois 60208, United States., Goo YA; Department of Chemistry, the Proteomics Center of Excellence, Northwestern University, Evanston, Illinois 60208, United States., Jewett MC; Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois 60208, United States., Rosenzweig AC; Department Molecular and Biological Sciences, Northwestern University, Evanston, Illinois 60208, United States., Kelleher NL; Department of Chemistry, the Proteomics Center of Excellence, Northwestern University, Evanston, Illinois 60208, United States.; Department Molecular and Biological Sciences, Northwestern University, Evanston, Illinois 60208, United States.

Publikováno v: ACS chemical biology [ACS Chem Biol] 2022 Oct 21; Vol. 17 (10), pp. 2769-2780. Date of Electronic Publication: 2022 Aug 11.

The enzymology of oxazolone and thioamide synthesis in methanobactin.

Autor: Chou JC; Department of Chemistry, Stanford University, Stanford, CA, United States., Stafford VE; Department of Chemistry, Stanford University, Stanford, CA, United States., Kenney GE; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, United States. Electronic address: gkenney@fas.harvard.edu., Dassama LMK; Department of Chemistry, Stanford University, Stanford, CA, United States; ChEM-H Institute, Stanford University, Stanford, CA, United States. Electronic address: dassama@stanford.edu.

Publikováno v: Methods in enzymology [Methods Enzymol] 2021; Vol. 656, pp. 341-373. Date of Electronic Publication: 2021 May 07.

A Genomic Toolkit for the Mechanistic Dissection of Intractable Human Gut Bacteria.

Autor: Bisanz JE; Department of Microbiology and Immunology, University of California San Francisco, San Francisco, CA 94143, USA., Soto-Perez P; Department of Microbiology and Immunology, University of California San Francisco, San Francisco, CA 94143, USA., Noecker C; Department of Microbiology and Immunology, University of California San Francisco, San Francisco, CA 94143, USA., Aksenov AA; Collaborative Mass Spectrometry Innovation Center, Department of Pediatrics, Center for Microbiome Innovation, Department of Pharmacology, Skaggs School of Pharmacy and Pharmaceutical Sciences, San Diego, CA 92093, USA., Lam KN; Department of Microbiology and Immunology, University of California San Francisco, San Francisco, CA 94143, USA., Kenney GE; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA., Bess EN; Department of Microbiology and Immunology, University of California San Francisco, San Francisco, CA 94143, USA., Haiser HJ; Faculty of Arts and Sciences Center for Systems Biology, Harvard University, Cambridge, MA 02138, USA., Kyaw TS; Department of Microbiology and Immunology, University of California San Francisco, San Francisco, CA 94143, USA., Yu FB; Chan Zuckerberg Biohub, San Francisco, CA 94158, USA., Rekdal VM; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA., Ha CWY; Department of Medicine, Division of Gastroenterology, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA., Devkota S; Department of Medicine, Division of Gastroenterology, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA., Balskus EP; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA., Dorrestein PC; Collaborative Mass Spectrometry Innovation Center, Department of Pediatrics, Center for Microbiome Innovation, Department of Pharmacology, Skaggs School of Pharmacy and Pharmaceutical Sciences, San Diego, CA 92093, USA., Allen-Vercoe E; Molecular and Cellular Biology, University of Guelph, Guelph, ON N1G 2W1, Canada., Turnbaugh PJ; Department of Microbiology and Immunology, University of California San Francisco, San Francisco, CA 94143, USA; Chan Zuckerberg Biohub, San Francisco, CA 94158, USA. Electronic address: peter.turnbaugh@ucsf.edu.

Publikováno v: Cell host & microbe [Cell Host Microbe] 2020 Jun 10; Vol. 27 (6), pp. 1001-1013.e9. Date of Electronic Publication: 2020 Apr 28.

Native top-down mass spectrometry provides insights into the copper centers of membrane-bound methane monooxygenase.

Autor: Ro SY; Departments of Molecular Biosciences and of Chemistry, Northwestern University, 2205 Tech Drive, Evanston, IL, 60208, USA., Schachner LF; Departments of Molecular Biosciences and of Chemistry, Northwestern University, 2205 Tech Drive, Evanston, IL, 60208, USA., Koo CW; Departments of Molecular Biosciences and of Chemistry, Northwestern University, 2205 Tech Drive, Evanston, IL, 60208, USA., Purohit R; Departments of Molecular Biosciences and of Chemistry, Northwestern University, 2205 Tech Drive, Evanston, IL, 60208, USA., Remis JP; Departments of Molecular Biosciences and of Chemistry, Northwestern University, 2205 Tech Drive, Evanston, IL, 60208, USA., Kenney GE; Departments of Molecular Biosciences and of Chemistry, Northwestern University, 2205 Tech Drive, Evanston, IL, 60208, USA., Liauw BW; Departments of Molecular Biosciences and of Chemistry, Northwestern University, 2205 Tech Drive, Evanston, IL, 60208, USA., Thomas PM; Departments of Molecular Biosciences and of Chemistry, Northwestern University, 2205 Tech Drive, Evanston, IL, 60208, USA., Patrie SM; Departments of Molecular Biosciences and of Chemistry, Northwestern University, 2205 Tech Drive, Evanston, IL, 60208, USA., Kelleher NL; Departments of Molecular Biosciences and of Chemistry, Northwestern University, 2205 Tech Drive, Evanston, IL, 60208, USA. n-kelleher@northwestern.edu., Rosenzweig AC; Departments of Molecular Biosciences and of Chemistry, Northwestern University, 2205 Tech Drive, Evanston, IL, 60208, USA. amyr@northwestern.edu.

Publikováno v: Nature communications [Nat Commun] 2019 Jun 17; Vol. 10 (1), pp. 2675. Date of Electronic Publication: 2019 Jun 17.