Zobrazeno 1 - 10
of 222
pro vyhledávání: '"Kenneth S. Zaret"'
Autor:
Takeshi Katsuda, Jonathan H. Sussman, Kenji Ito, Andrew Katznelson, Salina Yuan, Naomi Takenaka, Jinyang Li, Allyson J. Merrell, Hector Cure, Qinglan Li, Reyaz Ur Rasool, Irfan A. Asangani, Kenneth S. Zaret, Ben Z. Stanger
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-20 (2024)
Abstract Tissue damage elicits cell fate switching through a process called metaplasia, but how the starting cell fate is silenced and the new cell fate is activated has not been investigated in animals. In cell culture, pioneer transcription factors
Externí odkaz:
https://doaj.org/article/b2c5a3c430084dd3bae1dbe3580360ff
Publikováno v:
Cell Reports, Vol 42, Iss 7, Pp 112748- (2023)
Summary: Pioneer transcription factors interact with nucleosomes to scan silent, compact chromatin, enabling cooperative events that modulate gene activity. While at a subset of sites pioneer factors access chromatin by assisted loading with other tr
Externí odkaz:
https://doaj.org/article/7cb5edf2ab92472a89edfb941b252068
Publikováno v:
STAR Protocols, Vol 2, Iss 3, Pp 100651- (2021)
Summary: The protocol allows for labeling nascent RNA without isolating nuclei. The cell-permeable uridine analog, 5-ethynyluridine (EU), is added to media to allow in vivo labeling of nascent transcripts. Cells are lysed, total RNA is collected, and
Externí odkaz:
https://doaj.org/article/caecef630f814af781ecd0e56e254780
Autor:
Kai Fu, Constantinos Chronis, Abdenour Soufi, Giancarlo Bonora, Miguel Edwards, Stephen T. Smale, Kenneth S. Zaret, Kathrin Plath, Matteo Pellegrini
Publikováno v:
BMC Genomics, Vol 19, Iss 1, Pp 1-13 (2018)
Abstract Background Both human and mouse fibroblasts can be reprogrammed to pluripotency with Oct4, Sox2, Klf4, and c-Myc (OSKM) transcription factors. While both systems generate pluripotency, human reprogramming takes considerably longer than mouse
Externí odkaz:
https://doaj.org/article/fc2c7469eda74555a062c011964461eb
Autor:
Jonathan Lerner, Pablo Aurelio Gómez-García, Ryan L. McCarthy, Zhe Liu, Melike Lakadamyali, Kenneth S. Zaret
Publikováno v:
STAR Protocols, Vol 1, Iss 3, Pp 100223- (2020)
Summary: This protocol provides a two-parameter analysis of single-molecule tracking (SMT) trajectories of Halo-tagged histones in living adherent cell lines and unveils a chromatin mobility landscape composed of five chromatin types, ranging from lo
Externí odkaz:
https://doaj.org/article/966ead73821146d3a315874c42706be8
Autor:
Jungsun Kim, John P. Hoffman, R. Katherine Alpaugh, Andrew D. Rhim, Maximilian Reichert, Ben Z. Stanger, Emma E. Furth, Antonia R. Sepulveda, Chao-Xing Yuan, Kyoung-Jae Won, Greg Donahue, Jessica Sands, Andrew A. Gumbs, Kenneth S. Zaret
Publikováno v:
Cell Reports, Vol 3, Iss 6, Pp 2088-2099 (2013)
Pancreatic ductal adenocarcinoma (PDAC) carries a dismal prognosis and lacks a human cell model of early disease progression. When human PDAC cells are injected into immunodeficient mice, they generate advanced-stage cancer. We hypothesized that if h
Externí odkaz:
https://doaj.org/article/28f5c995ec434d528115c08678b63091
Autor:
Palle Serup, Carsten Gustavsen, Tino Klein, Leah A. Potter, Robert Lin, Nandita Mullapudi, Ewa Wandzioch, Angela Hines, Ashley Davis, Christine Bruun, Nina Engberg, Dorthe R. Petersen, Janny M. L. Peterslund, Raymond J. MacDonald, Anne Grapin-Botton, Mark A. Magnuson, Kenneth S. Zaret
Publikováno v:
Disease Models & Mechanisms, Vol 5, Iss 6, Pp 956-966 (2012)
SUMMARY Extracellular signals in development, physiology, homeostasis and disease often act by regulating transcription. Herein we describe a general method and specific resources for determining where and when such signaling occurs in live animals a
Externí odkaz:
https://doaj.org/article/63f5b0186d674ef187de317844c569e5
Publikováno v:
Trends in Biochemical Sciences. 48:513-526
Autor:
James Eberwine, Junhyong Kim, Ron C. Anafi, Steven Brem, Maja Bucan, Stephen A. Fisher, M. Sean Grady, Amy E. Herr, David Issadore, Hyejoong Jeong, HyunBum Kim, Daeyeon Lee, Stanislav Rubakhin, Jai-Yoon Sul, Jonathan V. Sweedler, John A. Wolf, Kenneth S. Zaret, James Zou
Publikováno v:
Nat Methods
We argue that the study of single-cell subcellular organelle omics is needed to understand and regulate cell function. This requires and is being enabled by new technology development.
Autor:
Kenneth S. Zaret
Publikováno v:
Genes & Development. 37:63-68