Zobrazeno 1 - 10
of 107
pro vyhledávání: '"Kenneth D. Cole"'
Autor:
Zhiyong He, Edward J. Kwee, Megan H. Cleveland, Kenneth D. Cole, Sheng Lin-Gibson, Hua-Jun He
Publikováno v:
Scientific Reports, Vol 13, Iss 1, Pp 1-11 (2023)
Abstract Lentiviral vectors (LV) have proven to be powerful tools for stable gene delivery in both dividing and non-dividing cells. Approval of these LVs for use in clinical applications has been achieved by improvements in LV design. Critically impo
Externí odkaz:
https://doaj.org/article/10b4b1f1c0404f13893e3a242c506026
Autor:
Jordan Cheng, Marco Morselli, Wei-Lun Huang, You Jeong Heo, Thalyta Pinheiro-Ferreira, Feng Li, Fang Wei, David Chia, Yong Kim, Hua-Jun He, Kenneth D. Cole, Wu-Chou Su, Matteo Pellegrini, David T.W. Wong
Publikováno v:
iScience, Vol 25, Iss 7, Pp 104554- (2022)
Summary: Plasma cell-free DNA is being widely explored as a biomarker for clinical screening. Currently, methods are optimized for the extraction and detection of double-stranded mononucleosomal cell-free DNA of ∼160bp in length. We introduce uscfD
Externí odkaz:
https://doaj.org/article/5a72e7dc01a64710ae579d732998f46c
Autor:
Barbara S. Paugh, Lajos Baranyi, Andre Roy, Hua-Jun He, Lindsay Harris, Kenneth D. Cole, Moria Artlip, Caroline Raimund, Patricia S. Langan, Srikanta Jana, Rimas J. Orentas, Sheng Lin-Gibson, Winfried Krueger, Boro Dropulić
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
Abstract Lentiviral vectors (LV) have emerged as a robust technology for therapeutic gene delivery into human cells as advanced medicinal products. As these products are increasingly commercialized, there are concomitant demands for their characteriz
Externí odkaz:
https://doaj.org/article/3dd0bd30801246de9358d8be2c031336
Publikováno v:
BioTechniques, Vol 64, Iss 2, Pp 59-68 (2018)
We compared different methods (absorbance, fluorescent dye-binding, and digital PCR) for measuring the concentrations of human genomic DNA from cultured cells and absorbance measurements of a synthetic DNA oligonucleotide. NIST Standard Reference Mat
Externí odkaz:
https://doaj.org/article/1bfa7a0c0a9a41039b6433f2712f88fb
Autor:
Kenneth D. Cole
Publikováno v:
BioTechniques, Vol 29, Iss 6, Pp 1256-1262 (2000)
A database was developed to store, organize, and retrieve the data associated with electrophoresis and chromatography separations. It allows laboratories to store extensive data on separation techniques (analytical and preparative). The data for gel
Externí odkaz:
https://doaj.org/article/f3e412d834274610bdf134dd17e5796f
Autor:
Kenneth D. Cole
Publikováno v:
BioTechniques, Vol 26, Iss 4, Pp 748-756 (1999)
Here, the application of the gel-forming carbohydrate polymer, gellan gum, for the electrophoresis and isolation of DNA is detailed. Gellan gum forms gels in the presence of divalent metal cations, and the gels can be converted back to a solution by
Externí odkaz:
https://doaj.org/article/800ee41889c645a7a9061037a7b84e56
Autor:
Henrikas Nivinskas, Kenneth D. Cole
Publikováno v:
BioTechniques, Vol 20, Iss 3, Pp 380-385 (1996)
Externí odkaz:
https://doaj.org/article/349488a4be2542998451c334667a7cbb
Autor:
Robert T. McCormack, Daniel Stetson, Dana E. Connors, J. Carl Barrett, Kenneth D. Cole, Steven P. Lund, Chris Karlovich, Thomas Forbes, P. Mickey Williams, Megan H. Cleveland, Laura M. Yee, Christie J. Lau, Cloud P. Paweletz, Gary J. Kelloff, Benoit Destenaves, Hua-Jun He, Susan M. Keating
Publikováno v:
JCO Precision Oncology
PURPOSEWe report the results from a Foundation for the National Institutes of Health Biomarkers Consortium project to address the absence of well-validated quality control materials (QCMs) for circulating tumor DNA (ctDNA) testing. This absence is co
Autor:
Wei-Lun Huang, David Chia, Wu Chou Su, You Jeong Heo, Hua Jun, David T.W. Wong, Feng Li, Fang Wei, Thalyta Pinheiro-Ferreira, Kenneth D. Cole, Marco Morselli, Matteo Pellegrini, Jordan Cheng
Plasma cell-free DNA is a widely used biomarker for diagnostic screening. We introduce uscfDNA-seq, a single-stranded cell-free DNA NGS pipeline, which bypasses previous limitations to reveal a novel population of ultrashort single-stranded cell-free
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::001509a7d18e906f10bf3198650ea6ad
https://doi.org/10.1101/2021.08.22.457243
https://doi.org/10.1101/2021.08.22.457243
Autor:
Kenneth D. Cole, Biswajit Das, Li Chen, P. Mickey Williams, Steve Lund, Megan H. Cleveland, Christopher R McEvoy, Jamie L. Almeida, Carolyn R. Steffen, Corinne Camalier, Liang-Chun Liu, Kara L. Norman, Chris Karlovich, Hua-Jun He, Andrew Fellowes
Publikováno v:
Clinical chemistry and laboratory medicine
Background The National Institute of Standards and Technology (NIST) Reference Material RM 8366 was developed to improve the quality of gene copy measurements of EGFR (epidermal growth factor receptor) and MET (proto-oncogene, receptor tyrosine kinas