Výsledky vyhledávání - "Kenji Mitsushima"

Large-scale production of functional human adrenomedullin: expression, cleavage, amidation, and purification

Autor: Kazuo Kitamura, Kenji Mitsushima, Shigeki Kamitani, Mitsuda Yuuichi, Akio Takimoto, Tsuneaki Sakata

Publikováno v: Protein Expression and Purification. 25:448-455

Human adrenomedullin (hAM) is a 52-amino-acid regulatory peptide containing a six-membered ring structure and an amidated C-terminus, features that are essential for its biological activity. Here, we describe a simple and effective protocol for produ

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f798ce21d872d3d8c9f009b2dc424c08
https://doi.org/10.1016/s1046-5928(02)00030-x

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High-level production of recombinant glutamic acid-specific protease from Bacilllus licheniformis in Bacillus subtilis expression system

Autor: Hiroshige Tsuzuki, Hideo Iwamoto, Kenji Mitsushima, Mikio Tamaki, Hiroyuki Okamoto, Shinji Kakudo, Hiroshi Teraoka, Koichi Matsumoto

Publikováno v: Journal of Fermentation and Bioengineering. 79:23-27

To obtain large quantities of glutamic acid-specific protease isolated originally from Bacillus licheniformis (BLase), an expression plasmid was constructed by inserting the BLase gene into a plasmid vector (pUB110) for Bacillus subtilis . B. subtili

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::299ad4ca0665175dc2bd1521d4961953
https://doi.org/10.1016/0922-338x(95)92738-x

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Purification, characterization and partial amino acid sequences of a novel cephalosporin-C deacetylase from Bacillus subtilis

Autor: Shigeo Yagi, Akio Takimoto, Kenji Mitsushima, Takayasu Sonoyama

Publikováno v: Journal of Fermentation and Bioengineering. 77:17-22

Cephalosporin-C deacetylase [EC 3.1.1.41] was purified electrophoretically to homogeneity from the newly isolated Bacillus subtilis SHS 0133 (FERM BP-2755). The enzyme was purified about 27-fold with a yield of 9 % and a specific activity of 187.4 U/

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::b6e55adc2ed2c2495e43dd19d60a9006
https://doi.org/10.1016/0922-338x(94)90201-1

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Batch production of deacetyl 7-aminocephalosporanic acid by immobilized cephalosporin-C deacetylase

Autor: Shigeo Yagi, Hiroyoshi Tani, Tomoaki Takakura, Kenji Mitsushima, Akio Takimoto

Publikováno v: Applied microbiology and biotechnology. 65(3)

Bacillus subtilis SHS0133 cephalosporin-C deacetylase (CAH) overexpressed in Escherichia coli was immobilized on an anion-exchange resin, KA-890, using glutaraldehyde. The activity yield of immobilized enzyme was approximately 55% of the free enzyme.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1b33f0a9c5824d8e0f456556906e18d4
https://pubmed.ncbi.nlm.nih.gov/15069587

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In vivo efficacy of recombinant methioninase is enhanced by the combination of polyethylene glycol conjugation and pyridoxal 5'-phosphate supplementation

Autor: Xinghua, Sun, Zhijian, Yang, Shukuan, Li, Yuying, Tan, Nan, Zhang, Xiaoen, Wang, Shigeo, Yagi, Takayuki, Yoshioka, Akio, Takimoto, Kenji, Mitsushima, Akinori, Suginaka, Eugene P, Frenkel, Robert M, Hoffman

Publikováno v: Cancer research. 63(23)

Recombinant methioninase (rMETase) is an enzyme active in preclinical mouse models of human cancer. The efficacy of rMETase is due to depletion of plasma methionine, an amino acid for which tumors generally have an abnormally high methionine requirem

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::777437ffcf60736892610f7af5d747a3
https://pubmed.ncbi.nlm.nih.gov/14678999

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Functional analysis of recombinant pancreatic secretory trypsin inhibitor protein with amino-acid substitution

Autor: Masanori Nakae, Masahiko Hirota, Shoji Nishihara, Hiroyuki Shimizu, Akio Takimoto, Kinuko Kuwata, Kenji Mitsushima, Michio Ogawa, Norihisa Kikuchi, Kazuaki Endo, Masayasu Inoue

Publikováno v: Journal of gastroenterology. 37(11)

We hypothesized that mutation of the pancreatic secretory trypsin inhibitor (PSTI) gene may promote a predisposition to pancreatitis, possibly by reducing the inhibition of trypsin activity. Based on this hypothesis, we performed a biochemical analys

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::97f45fd1c1f19a498e31f3b43119043d
https://pubmed.ncbi.nlm.nih.gov/12483248

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High-level expression, purification, and some properties of a recombinant cephalosporin-C deacetylase

Autor: Shigeo Yagi, Kenji Mitsushima, Akio Takimoto

Publikováno v: Journal of bioscience and bioengineering. 87(4)

To maximize the expression of the cephalosporin-C deacetylase (CAH) gene isolated from Bacillus subtilis SHS 0133 in Escherichia coli, a series of expression plasmids was constructed with various spacings between the Shine-Dalgarno sequence and the A

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1df19af2e7bdfe7f7c5812b988fb834a
https://pubmed.ncbi.nlm.nih.gov/16232499

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Gene cloning, nucleotide sequence, and expression of a cephalosporin-C deacetylase from Bacillus subtilis

Autor: Takayasu Sonoyama, A Takimoto, Kenji Mitsushima, Shigeo Yagi

Publikováno v: Applied and environmental microbiology. 61(6)

The gene encoding a cephalosporin-C deacetylase (CAH) from Bacillus subtilis SHS 0133 was cloned and sequenced. The nucleotide sequence contained an open reading frame encoding a polypeptide consisting of 318 amino acids, the molecular weight of whic

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d13bffa8c1342bb88844c66d66eec3c1
https://pubmed.ncbi.nlm.nih.gov/7793942

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