Establishment of human immortalized mesenchymal stem cells lines for the monitoring and analysis of osteogenic differentiation in living cells

Autor: Takashi Narai, Ryohei Watase, Yuji Nakayama, Isamu Kodani, Toshiaki Inoue, Kenji Kokura

Publikováno v: Heliyon, Vol 6, Iss 10, Pp e05398- (2020)

Mesenchymal stem cells (MSCs) are expected to be useful in bone regeneration treatment for various diseases and conditions, including cleft lip and palate, fracture, and bone absorption. However, to date, MSCs have failed to produce satisfactory resu

Externí odkaz: https://doaj.org/article/193cdc44d77c4bdd97c2f09bdc806832

Cisplatin Induces Cell Death in Rat Adult Kidney Stem/ Progenitor Cell-Derived Kidney Organoids

Autor: Yasushi Kuromi, Shinji Kitamura, Takeshi Endo, Shota Ueno, Kenji Kokura, Tetsuya Ohbayashi

Publikováno v: Yonago Acta Med
Yonago Acta Medica. 2023, 66(1), 153-158. doi:10.33160/yam.2023.02.018

Background: The use of stem/ progenitor cell-derived organoids to evaluate the toxicity of chemical substances has widely increased. Organoids with nephron-like structures (NLS) can be derived from rat adult kidney stem/ progenitor cells (rKS cells)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cf98d4cae67e8b4a1e671c42c4ee36fe
https://europepmc.org/articles/PMC9937970/

Stabilization of P/CAF, as a ubiquitin ligase toward MDM2, suppresses mitotic cell death through p53-p21 activation in HCT116 cells with SIRT2 suppression

Autor: Yanze Li, Kenji Kokura, Toshiaki Inoue

Publikováno v: This work was published by Elsevier : Li, Yanze, Kenji Kokura, and Toshiaki Inoue. "Stabilization of P/CAF, as a ubiquitin ligase toward MDM2, suppresses mitotic cell death through p53-p21 activation in HCT116 cells with SIRT2 suppression." Biochemical an

We previously reported that the suppression of SIRT2, an NAD + -dependent protein deacetylases, induces p53 accumulation via degradation of p300 and the subsequent MDM2 degradation, eventually leading to apoptosis in HeLa cells. The present study ide

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6ac50ea36832ec793f913340234a98c5
http://repository.lib.tottori-u.ac.jp/6220

Multiple expression cassette exchange via TP901-1, R4, and Bxb1 integrase systems on a mouse artificial chromosome

Autor: Kosuke Tomimatsu, Yuki Yoshimura, Tetsuya Ohbayashi, Kenji Kokura, Masashi Narita, Tadashi Nishida, Mitsuo Oshimura, Yasuhiro Kazuki

Publikováno v: FEBS Open Bio

The site‐specific excision of a target DNA sequence for genetic knockout or lineage tracing is a powerful tool for investigating biological systems. Currently, site‐specific recombinases (SSRs), such as Cre or Flp recombination target cassettes,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::180b8b64cdf2b8b4690052dcb0013cf8
https://doi.org/10.1002/2211-5463.12169

Evaluation of an Hprt-Luciferase Reporter Gene on a Mammalian Artificial Chromosome in Response to Cytotoxicity

Autor: Takeshi, Endo, Natsumi, Noda, Yasushi, Kuromi, Kenji, Kokura, Yasuhiro, Kazuki, Mitsuo, Oshimura, Tetsuya, Ohbayashi

Publikováno v: Yonago acta medica. 59(2)

Hypoxanthine guanine phosphoribosyltransferase (Hprt) is known as a house-keeping gene, and has been used as an internal control for real-time quantitative RT-PCR and various other methods of gene expression analysis. To evaluate the Hprt mRNA levels

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::60b05a28f08a612f162191454015f993
https://pubmed.ncbi.nlm.nih.gov/27493490