Kidney organoid derived from renal tissue stem cells is a useful tool for histopathological assessment of nephrotoxicity in a cisplatin-induced acute renal tubular injury model.

Autor: Shota Ueno^1,2 sueno@mail.kikkoman.co.jp, Kenji Kokura³, Yasushi Kuromi⁴, Mitsuhiko Osaki^1,5, Futoshi Okada^1,5, Shinji Kitamura⁶, Tetsuya Ohbayashi⁴

Publikováno v: Journal of Toxicologic Pathology. 2022, Vol. 35 Issue 4, p333-343. 11p.

Cisplatin Induces Cell Death in Rat Adult Kidney Stem/ Progenitor Cell-Derived Kidney Organoids

Autor: Yasushi Kuromi, Shinji Kitamura, Takeshi Endo, Shota Ueno, Kenji Kokura, Tetsuya Ohbayashi

Publikováno v: Yonago Acta Med
Yonago Acta Medica. 2023, 66(1), 153-158. doi:10.33160/yam.2023.02.018

Background: The use of stem/ progenitor cell-derived organoids to evaluate the toxicity of chemical substances has widely increased. Organoids with nephron-like structures (NLS) can be derived from rat adult kidney stem/ progenitor cells (rKS cells)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cf98d4cae67e8b4a1e671c42c4ee36fe
https://europepmc.org/articles/PMC9937970/

Stabilization of P/CAF, as a ubiquitin ligase toward MDM2, suppresses mitotic cell death through p53-p21 activation in HCT116 cells with SIRT2 suppression

Autor: Yanze Li, Kenji Kokura, Toshiaki Inoue

Publikováno v: This work was published by Elsevier : Li, Yanze, Kenji Kokura, and Toshiaki Inoue. "Stabilization of P/CAF, as a ubiquitin ligase toward MDM2, suppresses mitotic cell death through p53-p21 activation in HCT116 cells with SIRT2 suppression." Biochemical an

We previously reported that the suppression of SIRT2, an NAD + -dependent protein deacetylases, induces p53 accumulation via degradation of p300 and the subsequent MDM2 degradation, eventually leading to apoptosis in HeLa cells. The present study ide

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6ac50ea36832ec793f913340234a98c5
http://repository.lib.tottori-u.ac.jp/6220

Multiple expression cassette exchange via TP901-1, R4, and Bxb1 integrase systems on a mouse artificial chromosome

Autor: Kosuke Tomimatsu, Yuki Yoshimura, Tetsuya Ohbayashi, Kenji Kokura, Masashi Narita, Tadashi Nishida, Mitsuo Oshimura, Yasuhiro Kazuki

Publikováno v: FEBS Open Bio

The site‐specific excision of a target DNA sequence for genetic knockout or lineage tracing is a powerful tool for investigating biological systems. Currently, site‐specific recombinases (SSRs), such as Cre or Flp recombination target cassettes,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::180b8b64cdf2b8b4690052dcb0013cf8
https://doi.org/10.1002/2211-5463.12169

Evaluation of an Hprt-Luciferase Reporter Gene on a Mammalian Artificial Chromosome in Response to Cytotoxicity

Autor: Takeshi, Endo, Natsumi, Noda, Yasushi, Kuromi, Kenji, Kokura, Yasuhiro, Kazuki, Mitsuo, Oshimura, Tetsuya, Ohbayashi

Publikováno v: Yonago acta medica. 59(2)

Hypoxanthine guanine phosphoribosyltransferase (Hprt) is known as a house-keeping gene, and has been used as an internal control for real-time quantitative RT-PCR and various other methods of gene expression analysis. To evaluate the Hprt mRNA levels

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::60b05a28f08a612f162191454015f993
https://pubmed.ncbi.nlm.nih.gov/27493490