Zobrazeno 1 - 10
of 23
pro vyhledávání: '"Kenichi Higashiyama"'
Autor:
Kenichi Higashiyama, Mari Sato, Masaaki Kawata, Shoko Nishihara, Shinya Sugimoto, Kazumi Hirano, Ken-ichi Okuda, Yosio Mori, Takeshi Namiki, Yoshimitsu Mizunoe, Kenji Sonomoto, Chikara Sato, Hidetoshi Nishiyama, Tatsuhiko Ebihara, Takaaki Kinoshita, Shunsuke Matsumoto, Mitsuo Suga
Publikováno v:
Microscopy and Microanalysis. 20:469-483
High-throughput immuno-electron microscopy is required to capture the protein–protein interactions realizing physiological functions. Atmospheric scanning electron microscopy (ASEM) allowsin situcorrelative light and electron microscopy of samples
Autor:
Jun Ogawa, Hiroshi Kawashima, Yuriko Hirano, Kenichi Higashiyama, Nozomu Kamada, Sakayu Shimizu, M. Nishihara, Kengo Akimoto, Eiji Sakuradani
Publikováno v:
Applied Microbiology and Biotechnology. 60:281-287
Enhanced production of 5,8,11-eicosatrienoic acid (Mead acid, 20:3omega9) was attained with a mutant fungus, Mortierella alpina JT-180, derived from delta12 desaturation activity-defective and delta6 desaturation activity-enhanced M. alpina M209-7. P
Publikováno v:
Biotechnology and Bioprocess Engineering. 7:252-262
The growing interest in the application of arachidonic acid (ARA) in various fields of health and dietary requirements has elicited much attention on the industrial production of ARA-containing oil by the cultivation ofMortierella fungi. For the indu
Autor:
Yoshio Tanaka, Kazuhisa Ono, Tsutomu Morinaga, Osamu Suzuki, Seiko Shigeta, Tsunehiro Aki, Kengo Akimoto, Katsuyuki Ishihara, Yumiko Nagahata, Kenichi Higashiyama, Shigeaki Fujikawa, Seiji Kawamoto
Publikováno v:
Journal of the American Oil Chemists' Society. 78:599-604
A filamentous fungus producing significant levels of arachidonic acid (AA, C20∶4n−6) was isolated from a freshwater pond sample and assigned to the species Mortierella alliacea. This strain, YN-15, accumulated AA mainly in the form of triglycerid
Publikováno v:
Journal of Bioscience and Bioengineering. 91:382-389
The influence of the consumed carbon to nitrogen (C/N) ratio on arachidonic acid (AA) production and mycelial morphology was investigated in cultures of Mortierella alpina using shake flasks and a fermentor. The consumed C/N ratio was varied from 5 t
Publikováno v:
Biotechnology and Bioengineering. 65:537-541
The dielectric behavior of the filamentous fungi Mortierella alpina SAM2104 and 1S-4, which produce polyunsaturated fatty acid enriched oil in the mycelia, was investigated. During the cultivation carried out in a 10-kL fermentor for 12-15 days, the
Publikováno v:
Biotechnology and Bioengineering. 63:442-448
Arachidonic acid (AA) production by Mortierella alpina 1 S-4 was investigated using a 50-L fermentor. In order to optimize the dissolved oxygen (DO) concentration and to investigate the effect of DO on morphology, cultivation was carried out under co
Image analysis of morphological change during arachidonic acid production by Mortierella alpina 1S-4
Publikováno v:
Journal of Bioscience and Bioengineering. 87:489-494
The changes in mycelial morphology during arachidonic acid (AA) production by Mortierella alpina 1S-4 were investigated using an image analysis system. Cultivation was performed in a 10-kl fermentor, and the culture broth was separated into two fract
Autor:
Yumi Minamigawa, Kenichi Higashiyama, Shigeaki Fujikawa, Yuji Shimada, Akio Sugihara, Kengo Akimoto, Yoshio Tominaga, Sadao Komemushi
Publikováno v:
Journal of the American Oil Chemists' Society. 75:1213-1217
An attempt was made to enrich arachidonic acid (AA) from Mortierella single-cell oil, which had an AA content of 25%. The first step involved the hydrolysis of the oil with Pseudomonas sp. lipase. A mixture of 2.5 g oil, 2.5 g water, and 4000 units (
Publikováno v:
Journal of the American Oil Chemists' Society. 75:1815-1819
The culture conditions for arachidonic acid (AA) production by Mortierella alpina 1S-4 were investigated by means of a morphological study with the aims of obtaining a high AA yield and scale-up. In a 50-L jar fermentor study, a medium containing 3.1