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pro vyhledávání: '"Ken Heuermann"'
Autor:
Ken Heuermann, Jennifer Cosgrove
Publikováno v:
BioTechniques, Vol 30, Iss 5, Pp 1142-1147 (2001)
Blue/white selection is the standard method for detecting a cloned DNA fragment. In the absence of an insert, uninterrupted expression of the vector-encodeda-complement of β-galactosidase (β-gal), results in the hydrolysis of X-gal (5-bromo-4-chlor
Externí odkaz:
https://doaj.org/article/6e2118915d8c4021be22ebc16123017e
Publikováno v:
BioTechniques, Vol 28, Iss 4, Pp 789-793 (2000)
Three FLAG epitopes have been incorporated into the mammalian expression vector pCMV-5 to create a transient expression vector, p3XFLAG-CMV-7. The vector was designed to express FLAG fusion proteins that can be detected at tenfold lower expression le
Externí odkaz:
https://doaj.org/article/70e935f6ddab4cdab9ea1e5418451f53
Autor:
Jennifer Cosgrove, Ken Heuermann
Publikováno v:
BioTechniques. 30:1142-1147
Blue/white selection is the standard method for detecting a cloned DNA fragment. In the absence of an insert, uninterrupted expression of the vector-encoded alpha-complement of beta-galactosidase (beta-gal), results in the hydrolysis of X-gal (5-brom
Publikováno v:
BioTechniques. 28(4)
Three FLAG epitopes have been incorporated into the mammalian expression vector pCMV-5 to create a transient expression vector, p3XFLAG-CMV-7. The vector was designed to express FLAG fusion proteins that can be detected at tenfold lower expression le