Zobrazeno 1 - 10
of 277
pro vyhledávání: '"Kelvin P, Lee"'
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 32, Iss 4, Pp 101383- (2024)
To better understand host cell protein (HCP) retention in adeno-associated virus (AAV) downstream processes, sequential window acquisition of all theoretical fragment ion mass spectra (SWATH-MS) was used to quantitatively profile residual HCPs for fo
Externí odkaz:
https://doaj.org/article/80fedb1420ec48118567e0e6bd3d78ef
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 32, Iss 3, Pp 101306- (2024)
Faster and more accurate analytical methods are needed to support the advancement of recombinant adeno-associated virus (rAAV) production systems. Recently, biolayer interferometry (BLI) has been developed for high-throughput AAV capsid titer measure
Externí odkaz:
https://doaj.org/article/61f39c286a5e4d5784a0d64c7f355237
Publikováno v:
BMC Biotechnology, Vol 23, Iss 1, Pp 1-13 (2023)
Abstract Background Mammalian cell lines are frequently used as protein expression hosts because of their ability to correctly fold and assemble complex proteins, produce them at high titers, and confer post-translational modifications (PTMs) critica
Externí odkaz:
https://doaj.org/article/0ef028fca7a140df8d03a8cc0206cd78
Autor:
Madolyn L. MacDonald, Kelvin H. Lee
Publikováno v:
BMC Bioinformatics, Vol 22, Iss 1, Pp 1-26 (2021)
Abstract Background To select the most complete, continuous, and accurate assembly for an organism of interest, comprehensive quality assessment of assemblies is necessary. We present a novel tool, called Evaluation of De Novo Assemblies (EvalDNA), w
Externí odkaz:
https://doaj.org/article/9149ddb6466743b6a1005ecd98f92813
Autor:
Scott I Abrams, Audry Fernández, Liliana Oliver, Rydell Alvarez, Leslie Pérez, Circe Mesa, Kevin H Eng, Raquel Diaz, Anet Valdés, Sean H Colligan, Michael J Nemeth, Danielle Y F Twum, Olivia Fernández-Medina, Louise M Carlson, Han Yu, Mary L Hensen, Maura L Rábade-Chediak, Luis Enrique Fernández, Kelvin P Lee, Jason B Muhitch
Publikováno v:
Journal for ImmunoTherapy of Cancer, Vol 10, Iss 9 (2022)
Background Immune suppression is common in neoplasia and a major driver is tumor-induced myeloid dysfunction. Yet, overcoming such myeloid cell defects remains an untapped strategy to reverse suppression and improve host defense. Exposure of bone mar
Externí odkaz:
https://doaj.org/article/47e829035a71457082f388bc215f48d3
Publikováno v:
Frontiers in Oncology, Vol 12 (2022)
Multiple myeloma (MM) is an incapacitating hematological malignancy characterized by accumulation of cancerous plasma cells in the bone marrow (BM) and production of an abnormal monoclonal protein (M-protein). The BM microenvironment has a key role i
Externí odkaz:
https://doaj.org/article/93f18b0737f542da94b3ffea6fb665c9
Autor:
Sumit K. Singh, Kelvin H. Lee
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 9 (2022)
Glycosylation is a critical quality attribute of monoclonal antibody (mAb) therapeutics. Hydrophilic interaction liquid chromatography-mass spectrometry (HILIC-MS) is an invaluable technology for the characterization of protein glycosylation. HILIC/M
Externí odkaz:
https://doaj.org/article/6bd1380ac877487d934d5461a0477b36
Autor:
Peter Bloomingdale, Cristina Meregalli, Kevin Pollard, Annalisa Canta, Alessia Chiorazzi, Giulia Fumagalli, Laura Monza, Eleonora Pozzi, Paola Alberti, Elisa Ballarini, Norberto Oggioni, Louise Carlson, Wensheng Liu, Mehrnoosh Ghandili, Tracey A. Ignatowski, Kelvin P. Lee, Michael J. Moore, Guido Cavaletti, Donald E. Mager
Publikováno v:
Frontiers in Pharmacology, Vol 12 (2022)
Chemotherapy-induced peripheral neurotoxicity is a common dose-limiting side effect of several cancer chemotherapeutic agents, and no effective therapies exist. Here we constructed a systems pharmacology model of intracellular signaling in peripheral
Externí odkaz:
https://doaj.org/article/1ccf464a944a4141a28474e8a0809c1d
Autor:
Seyeon Bae, Peter Sang Uk Park, Yeji Lee, Se Hwan Mun, Eugenia Giannopoulou, Takayuki Fujii, Kelvin P. Lee, Sara Nunes Violante, Justin R. Cross, Kyung-Hyun Park-Min
Publikováno v:
Cell Reports, Vol 35, Iss 11, Pp 109264- (2021)
Summary: MYC activates different metabolic programs in a cell-type- and cell-status-dependent manner. However, the role of MYC in inflammatory macrophages has not yet been determined. Metabolic and molecular analyses reveal that MYC, but not hypoxia
Externí odkaz:
https://doaj.org/article/f1316364c7fb416295e88be27e4869c4
Publikováno v:
mSphere, Vol 6, Iss 2 (2021)
Next-generation sequencing (NGS) has been proposed as a complementary method to detect adventitious viruses in the production of biotherapeutics and vaccines to current in vivoin vitro
Externí odkaz:
https://doaj.org/article/f3042c6702294b7c8e929777cc87ed15