Zobrazeno 1 - 10
of 30
pro vyhledávání: '"Kelvin J Liu"'
Autor:
Yunke Song, Duncan Kilburn, Jee Hoon Song, Yulan Cheng, Christopher T Saeui, Douglas G Cheung, Carlo M Croce, Kevin J Yarema, Stephen J Meltzer, Kelvin J Liu, Tza-Huei Wang
Publikováno v:
PLoS ONE, Vol 12, Iss 7, p e0180988 (2017)
Accurate measurement of miRNA expression is critical to understanding their role in gene expression as well as their application as disease biomarkers. Correct identification of changes in miRNA expression rests on reliable normalization to account f
Externí odkaz:
https://doaj.org/article/a94eaca396c447fa8071bc2f22837837
Publikováno v:
PLoS ONE, Vol 9, Iss 4, p e94619 (2014)
Adapter ligation is a critical first step in many microRNA analysis methods including microarray, qPCR, and sequencing. Previous studies have shown that ligation bias can have dramatic effects on both the fidelity of expression profiles and reproduci
Externí odkaz:
https://doaj.org/article/e12213e1ba7d45b8ba0bca3303a6382a
Autor:
M. B. Couger, Stacy Pirro, Scott William Roy, Duncan Kilburn, Todd M. Wilson, Polly Campbell, Kelvin J. Liu, Laurie Dizney, Landen Gozashti, Luis A. Ruedas, Clinton W. Epps, Michelle Kim, Lindsay S. Millward, Noelle Anderson
Publikováno v:
Science. 372:592-600
The mammalian sex chromosome system (XX female/XY male) is ancient and highly conserved. The sex chromosome karyotype of the creeping vole (Microtus oregoni) represents a long-standing anomaly, with an X chromosome that is unpaired in females (X0) an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f00321fa52556b798ab635248a88f625
https://doi.org/10.1126/science.abg7019
https://doi.org/10.1126/science.abg7019
Nanopore sequencing and the Shasta toolkit enable efficient de novo assembly of eleven human genomes
Autor:
Karen H. Miga, Miten Jain, Simon Mayes, Kristof Tigyi, Kishwar Shafin, Benedict Paten, Nicholas Maurer, Ryan Lorig-Roach, Colleen M. Bosworth, Justin M. Zook, Duncan Kilburn, Hugh E. Olsen, Vania Costa, David Haussler, Kelvin J. Liu, Richard E. Green, Tobias Marschall, Melanie Sorensen, Paolo Carnevali, Sofie R. Salama, Erik Garrison, Mark Akeson, Marina Haukness, Mitchell R. Vollger, Katherine M. Munson, Fritz J. Sedlazeck, Adam M. Phillippy, Joel Armstrong, Jean Monlong, Sergey Koren, Evan E. Eichler, Trevor Pesout
Publikováno v:
Nature biotechnology, vol 38, iss 9
Nature Biotechnology
Nature biotechnology
Nature Biotechnology
Nature biotechnology
De novo assembly of a human genome using nanopore long-read sequences has been reported but it used more than 150,000 CPU hours and weeks of wall-clock time. To enable rapid human genome assembly we present Shasta, a de novo long read assembler, and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9a270be65d4f80570a6f846fec58819c
https://doi.org/10.1038/s41587-020-0503-6
https://doi.org/10.1038/s41587-020-0503-6
Publikováno v:
Nature Communications, Vol 8, Iss 1, Pp 1-10 (2017)
Nature Communications
Nature Communications
In-line preconcentration techniques are used to improve the sensitivity of microfluidic DNA analysis platforms. The most common methods are electrokinetic and require an externally applied electric field. Here we describe a microfluidic DNA preconcen
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e2fd750ef80f4d743a59a679bf4a4d77
https://doaj.org/article/be96a85d1e7e4c87a20d2f0eaaddda44
https://doaj.org/article/be96a85d1e7e4c87a20d2f0eaaddda44
Autor:
Nicholas Maurer, Karen H. Miga, Hugh E. Olsen, Tobias Marschall, Kelvin J. Liu, Colleen M. Bosworth, Kristof Tigyi, Simon Mayes, Joel Armstrong, Mark Akeson, Benedict Paten, Evan E. Eichler, Trevor Pesout, Marina Haukness, Katherine M. Munson, Fritz J. Sedlazeck, Sofie R. Salama, Costa, Richard E. Green, Duncan Kilburn, Melanie Sorensen, Paolo Carnevali, Miten Jain, Adam M. Phillippy, Ryan Lorig-Roach, Sergey Koren, Mitchell R. Vollger, Kishwar Shafin, David Haussler, Justin M. Zook
Present workflows for producing human genome assemblies from long-read technologies have cost and production time bottlenecks that prohibit efficient scaling to large cohorts. We demonstrate an optimized PromethION nanopore sequencing method for elev
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ec6c77402585d576fe0061cbb7238ca6
Autor:
Kelvin J. Liu, Sarah M. Friedrich, Tza-Huei Wang, Jeffrey M. Burke, Ye Zhang, Yi Zhang, Kristin Rule Gleitsman
Publikováno v:
Advanced Materials. 28:10630-10636
An inexpensive, magnetic thermoplastic nanomaterial is developed utilizing a hierarchical layering of micro- and nanoscale silica lamellae to create a high-surface-area and low-shear substrate capable of capturing vast amounts of ultrahigh-molecular-
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f46af2f0b3d4790fee03104855906dff
https://doi.org/10.1002/adma.201603738
https://doi.org/10.1002/adma.201603738
Autor:
Duncan Kilburn, Stephen J. Meltzer, Yulan Cheng, Yunke Song, Carlo M. Croce, Tza-Huei Wang, Christopher T. Saeui, Douglas G. Cheung, Jee Hoon Song, Kevin J. Yarema, Kelvin J. Liu
Publikováno v:
PLoS ONE, Vol 12, Iss 7, p e0180988 (2017)
PLoS ONE
PLoS ONE
Accurate measurement of miRNA expression is critical to understanding their role in gene expression as well as their application as disease biomarkers. Correct identification of changes in miRNA expression rests on reliable normalization to account f
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3295b74fb7fe8853b131d8aa919ee8c6
http://europepmc.org/articles/PMC5509254?pdf=render
http://europepmc.org/articles/PMC5509254?pdf=render
Publikováno v:
Nano Letters
Nonviral gene delivery holds great promise not just as a safer alternative to viral vectors in traditional gene therapy applications, but also for regenerative medicine, induction of pluripotency in somatic cells, and RNA interference for gene silenc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d04595ff6baf56ea30661adc6226b959
http://europepmc.org/articles/PMC4134141
http://europepmc.org/articles/PMC4134141
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1509
Ligo-miR is an assay technology that can perform multiplexed detection of miRNAs from a wide range of biological sources. At its core are two sequential ligation steps. First in the capture ligation, template molecules are created by ligating a DNA a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::5986630dc72d38364712f1b02997431a
https://pubmed.ncbi.nlm.nih.gov/27826928
https://pubmed.ncbi.nlm.nih.gov/27826928