Zobrazeno 1 - 10
of 17
pro vyhledávání: '"Kelvin F Cho"'
Autor:
Kelvin F Cho, Shawn M Gillespie, Nicholas A Kalogriopoulos, Michael A Quezada, Martin Jacko, Michelle Monje, Alice Y Ting
Publikováno v:
eLife, Vol 11 (2022)
Technologies for detecting cell-cell contacts are powerful tools for studying a wide range of biological processes, from neuronal signaling to cancer-immune interactions within the tumor microenvironment. Here, we report TRACC (Transcriptional Readou
Externí odkaz:
https://doaj.org/article/ba21240dbd5f4fa98d5721e296c4f39a
Publikováno v:
eLife, Vol 8 (2019)
Technologies that convert transient protein-protein interactions (PPIs) into stable expression of a reporter gene are useful for genetic selections, high-throughput screening, and multiplexing with omics technologies. We previously reported SPARK (Ki
Externí odkaz:
https://doaj.org/article/641acd926a6c41269c61c365c72d7c8c
Autor:
Kelvin F. Cho, Taylur P. Ma, Christopher M. Rose, Donald S. Kirkpatrick, Kebing Yu, Robert A. Blake
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
Quantitative profiling of small molecule-protein binding in cells can aid basic biochemical research and drug discovery. Here, the authors develop the Heat Shock Protein Inhibition Protein Stability Assay (HIPStA) as a high-throughput method to asses
Externí odkaz:
https://doaj.org/article/d8e4230757ef4fa5aa0391581b106a4b
Autor:
Song-Yi Lee, Joleen S. Cheah, Boxuan Zhao, Charles Xu, Heegwang Roh, Christina K. Kim, Kelvin F. Cho, Namrata D. Udeshi, Steven A. Carr, Alice Y. Ting
Publikováno v:
bioRxiv
The incorporation of light-responsive domains into engineered proteins has enabled control of protein localization, interactions, and function with light. We integrated optogenetic control into proximity labeling (PL), a cornerstone technique for hig
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::628372ce48b8cd89bd80b23ea0eed1a7
https://doi.org/10.1038/s41592-023-01880-5
https://doi.org/10.1038/s41592-023-01880-5
Publikováno v:
Nature Methods. 18:133-143
Many biological processes are executed and regulated through the molecular interactions of proteins and nucleic acids. Proximity labeling (PL) is a technology for tagging the endogenous interaction partners of specific protein ‘baits’, via geneti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::92696cb2d9d37c210f6a30ace9d95e92
https://doi.org/10.1038/s41592-020-01010-5
https://doi.org/10.1038/s41592-020-01010-5
Autor:
Kelvin F. Cho, Namrata D. Udeshi, Alice Y. Ting, Steven A. Carr, Tess C. Branon, Samuel A. Myers
Publikováno v:
Nature Protocols. 15:3971-3999
This protocol describes the use of TurboID and split-TurboID in proximity labeling applications for mapping protein–protein interactions and subcellular proteomes in live mammalian cells. TurboID is an engineered biotin ligase that uses ATP to conv
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::74d8f2df6a0b7c82c52c035f849cc5f5
https://doi.org/10.1038/s41596-020-0399-0
https://doi.org/10.1038/s41596-020-0399-0
Autor:
Alexander Bukreyev, Erica Ollmann Saphire, Colette Pietzsch, Alice Y. Ting, George Tsaprailis, Kelvin F. Cho, Jingru Fang, Gogce Crynen, Juan Carlos de la Torre
Ebola virus (EBOV) critically depends on the viral polymerase to replicate and transcribe the viral RNA genome. To examine whether interactions between EBOV polymerase and cellular and viral factors affect distinct viral RNA synthesis events, we appl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::01d681f79aa6f07dd5e29e7cc58f06f2
https://doi.org/10.1101/2021.07.20.453153
https://doi.org/10.1101/2021.07.20.453153
Autor:
Kelvin F Cho, Shawn M Gillespie, Nicholas A Kalogriopoulos, Michael A Quezada, Martin Jacko, Michelle Monje, Alice Y Ting
Publikováno v:
eLife. 11
Technologies for detecting cell-cell contacts are powerful tools for studying a wide range of biological processes, from neuronal signaling to cancer-immune interactions within the tumor microenvironment. Here, we report TRACC (Transcriptional Readou
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::310e485929fc4bbe0746d9c041ebe3f5
https://pubmed.ncbi.nlm.nih.gov/35311648
https://pubmed.ncbi.nlm.nih.gov/35311648
Autor:
George Tsaprailis, Alice Y. Ting, Alexander Bukreyev, Juan Carlos de la Torre, Gogce Crynen, Colette Pietzsch, Erica Ollmann Saphire, Kelvin F. Cho, Jingru Fang
Publikováno v:
SSRN Electronic Journal.
Ebola virus (EBOV) critically depends on the viral polymerase to replicate and transcribe the viral RNA genome. To examine whether interactions between EBOV polymerase and cellular and viral factors affect distinct viral RNA synthesis events, we appl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::365c9894018b47b1e07a3146eabcd73f
https://doi.org/10.2139/ssrn.3895930
https://doi.org/10.2139/ssrn.3895930
Publikováno v:
Nature methods. 18(2)
Many biological processes are executed and regulated through the molecular interactions of proteins and nucleic acids. Proximity labeling (PL) is a technology for tagging the endogenous interaction partners of specific protein 'baits', via genetic fu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::54be88458e0a0a77b067db758a99cd03
https://pubmed.ncbi.nlm.nih.gov/33432242
https://pubmed.ncbi.nlm.nih.gov/33432242