Výsledky vyhledávání

Akademický článek

Functional Gene Knockout of NRF2 Increases Chemosensitivity of Human Lung Cancer A549 Cells In Vitro and in a Xenograft Mouse Model

Autor: Pawel Bialk, Yichen Wang, Kelly Banas, Eric B. Kmiec

Publikováno v: Molecular Therapy: Oncolytics, Vol 11, Iss , Pp 75-89 (2018)

Recent studies point to the evolution of drug resistance in lung cancer as being centered, at least in part, on the upregulation of various genes involved in controlling efflux or drug inactivation. Among the most important of these genes is Nuclear

Externí odkaz: https://doaj.org/article/b08f67e4d39b4cea9416503519598f39

Zobrazit plný text záznamu

Akademický článek

Insertional Mutagenesis by CRISPR/Cas9 Ribonucleoprotein Gene Editing in Cells Targeted for Point Mutation Repair Directed by Short Single-Stranded DNA Oligonucleotides.

Autor: Natalia Rivera-Torres, Kelly Banas, Pawel Bialk, Kevin M Bloh, Eric B Kmiec

Publikováno v: PLoS ONE, Vol 12, Iss 1, p e0169350 (2017)

CRISPR/Cas9 and single-stranded DNA oligonucleotides (ssODNs) have been used to direct the repair of a single base mutation in human genes. Here, we examine a method designed to increase the precision of RNA guided genome editing in human cells by ut

Externí odkaz: https://doaj.org/article/d05a9e9964e243fc8fc758f9d43231a1

Zobrazit plný text záznamu

Figure S1, Figure S2, Figure S3 from Kinetics of Nuclear Uptake and Site-Specific DNA Cleavage during CRISPR-Directed Gene Editing in Solid Tumor Cells

Autor: Eric B. Kmiec, Byung-Chun Yoo, Pawel Bialk, Natalia Rivera-Torres, Kelly Banas

Figure S1. Fluorescence compensation of mock transfected, GFP- and ATTO 550-fluorescing cells. Figure S2. Representative images used for time course analysis. Figure S3. In vitro cleavage reaction of wildtype and R34G-mutated NRF2 amplicons with vary

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::63b3da7d77a5cb7115e48adbf58b0236
https://doi.org/10.1158/1541-7786.22513666.v1

Zobrazit plný text záznamu

Deconvolution of Complex DNA Repair (DECODR): Establishing a Novel Deconvolution Algorithm for Comprehensive Analysis of CRISPR-Edited Sanger Sequencing Data

Autor: Kevin Bloh, Zugui Zhang, Eric B. Kmiec, Pawel Bialk, Rohan Kanchana, Kelly Banas, Byung-Chun Yoo

Publikováno v: The CRISPR Journal

During CRISPR-directed gene editing, multiple gene repair mechanisms interact to produce a wide and largely unpredictable variety of sequence changes across an edited population of cells. Shortcomings inherent to previously available proposal-based i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b4ec2a9afaf32fee3c39465795c76613
http://europepmc.org/articles/PMC7898406

Zobrazit plný text záznamu

Modeling pediatric AML FLT3 mutations using CRISPR/Cas12a- mediated gene editing

Autor: Kelly Banas, Eric B. Kmiec, Natalia Rivera-Torres

Publikováno v: Leuk Lymphoma

Clustered regularly interspaced palindromic repeats (CRISPR) with the associated (Cas) nuclease complexes have democratized genetic engineering through their precision and ease-of-use. We have applied a variation of this technology, known as CRISPR-d

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cdf11696e720e8b08c9213f320092a5c
https://doi.org/10.1080/10428194.2020.1805740

Zobrazit plný text záznamu

Plný text ve formátu HTML

Kinetics of Nuclear Uptake and Site-Specific DNA Cleavage during CRISPR-Directed Gene Editing in Solid Tumor Cells

Autor: Eric B. Kmiec, Natalia Rivera-Torres, Pawel Bialk, Kelly Banas, Byung-Chun Yoo

Publikováno v: Mol Cancer Res

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)–directed gene editing is approaching clinical implementation in cancer. Thus, it is imperative to define the molecular framework upon which safe and efficacious therapeutic strategi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::72f70836966f6bb213b2e1a0f7f259b1
https://doi.org/10.1158/1541-7786.mcr-19-1208

Zobrazit plný text záznamu

Exon skipping induced by CRISPR-directed gene editing regulates the response to chemotherapy in non-small cell lung carcinoma cells

Autor: Kelly Banas, Shirin Modarai, Natalia Rivera-Torres, Byung-Chun Yoo, Pawel A. Bialk, Connor Barrett, Mona Batish, Eric B. Kmiec

Publikováno v: Gene therapy. 29(6)

We have been developing CRISPR-directed gene editing as an augmentative therapy for the treatment of non-small cell lung carcinoma (NSCLC) by genetic disruption of Nuclear Factor Erythroid 2-Related Factor 2 (NRF2). NRF2 promotes tumor cell survival

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f60abb70a164e4cc02343e9373d17dd7
https://pubmed.ncbi.nlm.nih.gov/35314779

Zobrazit plný text záznamu

Temporal analyses of CRISPR-directed gene editing on NRF2, a clinically relevant human gene involved in chemoresistance

Autor: Eric B. Kmiec, Byung-Chun Yoo, Natalia Rivera-Torres, Kelly Banas, Pawel Bialk

Recent data suggest that a time lag exists between nuclear penetration and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-directed gene editing activity in human cells. As CRISPR/Cas approaches clinical implementation, it is criti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c2d32a02f0323c9613e40ab3c426212c
https://doi.org/10.1101/799676

Zobrazit plný text záznamu

Insertional Mutagenesis by CRISPR/Cas9 Ribonucleoprotein Gene Editing in Cells Targeted for Point Mutation Repair Directed by Short Single-Stranded DNA Oligonucleotides

Autor: Kevin Bloh, Kelly Banas, Pawel Bialk, Eric B. Kmiec, Natalia Rivera-Torres

Publikováno v: PLoS ONE, Vol 12, Iss 1, p e0169350 (2017)
PLoS ONE

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3ebbc74e49a7b973b2aff0e056d987d8
https://doi.org/10.1371/journal.pone.0169350

Zobrazit plný text záznamu

Vyhledávací nástroje:

Upřesnit hledání