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pro vyhledávání: '"Kelli G. Kline"'
Publikováno v:
Current Opinion in Plant Biology. 14:507-511
Protein phosphorylation is a major post-translational modification in plants crucial for the regulation of diverse cellular functions. In the early stages of this field, efforts were focused on the qualitative detection, identification, and catalogin
Autor:
Marisa S. Otegui, Michael R. Sussman, Miyoshi Haruta, Jeffery C. Young, Rachel B. Nelson, Kelli G. Kline, Heather L. Burch, Sheher B. Mohsin, Greg Barrett-Wilt
Publikováno v:
Journal of Biological Chemistry. 285:17918-17929
Arabidopsis mutants containing gene disruptions in AHA1 and AHA2, the two most highly expressed isoforms of the Arabidopsis plasma membrane H(+)-ATPase family, have been isolated and characterized. Plants containing homozygous loss-of-function mutati
Publikováno v:
Analytical Chemistry. 82:3435-3440
Elevated chromatographic temperatures are well recognized to provide beneficial analytical effects. Previously, we demonstrated that elevated chromatographic temperature enhances the identification of hydrophobic peptides from enriched membrane sampl
Autor:
Kelli G. Kline, Michael R. Sussman
Publikováno v:
Annual Review of Biophysics. 39:291-308
Genetic, chemical, and environmental perturbations can all induce large changes in cellular proteomes, and research aimed at quantifying these changes are an important part of modern biology. Although improvements in the hardware and software of mass
Publikováno v:
Journal of Proteome Research. 7:5055-5061
Proteomics research is beginning to expand beyond the more traditional shotgun analysis of protein mixtures to include targeted analyses of specific proteins using mass spectrometry. Integral to the development of a robust assay based on targeted mas
Publikováno v:
Plant physiology. 154(2)
Abscisic acid (ABA), a sesquiterpenoid phytohormone, was first discovered in the 1960s, and has since been found to be a key regulator of such diverse processes as dormancy, germination, seed development, and responses to biotic and abiotic stress (C
Publikováno v:
Briefings in functional genomicsproteomics. 8(2)
The ultimate goal of most shotgun proteomic pipelines is the discovery of novel biomarkers to direct the development of quantitative diagnostics for the detection and treatment of disease. Differential comparisons of biological samples identify candi
Autor:
Kelli G. Kline, Christine C. Wu
Publikováno v:
Methods in Molecular Biology ISBN: 9781603273091
Although two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) has been used as the standard proteomic approach for separating proteins in a complex mixture, this technique has many drawbacks. These include a limited molecular mass range, poor