Zobrazeno 1 - 10
of 17
pro vyhledávání: '"Kelley M, Heffner"'
Autor:
Nathan E. Lewis, Michael A. Bowen, Paula Meleady, Seong Won Cha, Shangzhong Li, Deniz Baycin Hizal, Susan T. Sharfstein, Prashant Kaushik, Vijay Tejwani, Robert N. Cole, Michael J. Betenbaugh, Kelley M. Heffner, Michael Henry, Raghothama Chaerkady, Vineet Bafna
Publikováno v:
J Proteome Res
A high quality genome annotation greatly facilitates successful cell line engineering. Standard draft genome annotation pipelines are based largely onde novogene prediction, homology, and RNA-Seq data. However, draft annotations can suffer from incor
Autor:
Shangzhong Li, Oliver Rupp, Kelvin H. Lee, Shawn W. Polson, Madolyn L. MacDonald, Nicole Borth, Sven Griep, Haiping Hao, Kelley M. Heffner, Michael J. Betenbaugh, Mojtaba Samoudi, Nathan E. Lewis, Brewster F. Kingham, Inmaculada Hernandez, Vaibhav Jadhav, Alexander Goesmann, Heena Dhiman, Karina Brinkrolf
Publikováno v:
Biotechnology and bioengineering, vol 115, iss 8
Biotechnology and Bioengineering
Rupp, O; MacDonald, ML; Li, S; Dhiman, H; Polson, S; Griep, S; et al.(2018). A reference genome of the Chinese hamster based on a hybrid assembly strategy. BIOTECHNOLOGY AND BIOENGINEERING, 115(8), 2087-2100. doi: 10.1002/bit.26722. UC San Diego: Retrieved from: http://www.escholarship.org/uc/item/27b6z5c8
Biotechnology and Bioengineering
Rupp, O; MacDonald, ML; Li, S; Dhiman, H; Polson, S; Griep, S; et al.(2018). A reference genome of the Chinese hamster based on a hybrid assembly strategy. BIOTECHNOLOGY AND BIOENGINEERING, 115(8), 2087-2100. doi: 10.1002/bit.26722. UC San Diego: Retrieved from: http://www.escholarship.org/uc/item/27b6z5c8
Accurate and complete genome sequences are essential in biotechnology to facilitate genome‐based cell engineering efforts. The current genome assemblies for Cricetulus griseus, the Chinese hamster, are fragmented and replete with gap sequences and
Autor:
Albert E. Schmelzer, Chris Barton, David Spencer, Jeong H. Lee, Jason Reier, Raghavan Venkat, Kelley M. Heffner
Publikováno v:
Biotechnology and Bioengineering. 114:1991-2000
Coagulation factor II (prothrombin; FII) is the pre-proteolyzed precursor to thrombin in the coagulation cascade. It has 10 sites of gamma-carboxylation, which are required for its bioactivity, and is N-glycosylated at three of four putative sites. P
Autor:
Miao Wang, Xianlin Han, Joseph Priola, Kelley M. Heffner, Yue Zhang, Deniz Baycin-Hizal, Michelle Bahri, Amit Kumar, Michael J. Betenbaugh, Michael A. Bowen
Publikováno v:
Analytical Chemistry. 89:1477-1485
A combined lipidomics and transcriptomics analysis was performed on mouse myeloma SP2/0, Chinese hamster ovary (CHO), and human embryonic kidney (HEK) cells in order to compare widely used mammalian expression systems. Initial thin layer chromatograp
Autor:
Athena Yerganian, Robert N. O'Meally, Michael A. Bowen, Venkata Gayatri Dhara, Kelley M. Heffner, Swetha Kumar, Natalia I. Majewska, Robert Cole, George Yerganian, Michael J. Betenbaugh, Deniz Baycin Hizal, Diane Hatton, Jie Zhu
Publikováno v:
Scientific Reports
Scientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
Scientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
Chinese hamster ovary (CHO) cells are the predominant production vehicle for biotherapeutics. Quantitative proteomics data were obtained from two CHO cell lines (CHO-S and CHO DG44) and compared with seven Chinese hamster (Cricetulus griseus) tissues
Autor:
Michael J. Betenbaugh, Nathan E. Lewis, Michael A. Bowen, Deniz Baycin-Hizal, Raghothama Chaerkady, Amit Kumar, Daniel Wolozny, Kelley M. Heffner, Joseph Shiloach, Lasse Ebdrup Pedersen, Robert N. Cole, Hui Zhang
Publikováno v:
Journal of Proteome Research. 14:4687-4703
Chinese hamster ovary (CHO) cells are the preferred host cell line for manufacturing a variety of complex biotherapeutic drugs including monoclonal antibodies. We performed a proteomics and bioinformatics analysis on the spent medium from adherent CH
Publikováno v:
Advances in biochemical engineering/biotechnology. 175
Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics. M
Publikováno v:
Advances in Glycobiotechnology ISBN: 9783030695897
Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics. M
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::19ff015273ed7333c00289110c38eb3f
https://doi.org/10.1007/10_2017_57
https://doi.org/10.1007/10_2017_57
Autor:
Jie Zhu, Joseph Shiloach, Michael J. Betenbaugh, Michael A. Bowen, Amit Kumar, Kelley M. Heffner, Deniz Baycin Hizal
Publikováno v:
Curr Opin Biotechnol
Recent advancements in proteomics have enabled the generation of high-quality data sets useful for applications ranging from target and monoclonal antibody (mAB) discovery to bioprocess optimization. Comparative proteomics approaches have recently be
Publikováno v:
Pharmaceutical Bioprocessing. 2:421-435
Chinese hamster ovary (CHO) cell lines are the preferred host for manufacturing therapeutic proteins. The use of proteomics for recombinant protein production in CHO cells has expanded recently because of the ease in identifying protein levels and it