An acetylation-mediated chromatin switch governs H3K4 methylation read-write capability

Autor: Kanishk Jain, Matthew R Marunde, Jonathan M Burg, Susan L Gloor, Faith M Joseph, Karl F Poncha, Zachary B Gillespie, Keli L Rodriguez, Irina K Popova, Nathan W Hall, Anup Vaidya, Sarah A Howard, Hailey F Taylor, Laylo Mukhsinova, Ugochi C Onuoha, Emily F Patteson, Spencer W Cooke, Bethany C Taylor, Ellen N Weinzapfel, Marcus A Cheek, Matthew J Meiners, Geoffrey C Fox, Kevin EW Namitz, Martis W Cowles, Krzysztof Krajewski, Zu-Wen Sun, Michael S Cosgrove, Nicolas Young, Michael-C Keogh, Brian D Strahl

In nucleosomes, histone N-terminal tails exist in dynamic equilibrium between free/accessible and collapsed/DNA-bound states. The latter state is expected to impact histone N-termini availability to the epigenetic machinery. Notably, H3 tail acetylat

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::56293051dfd39cb6a2645e19d0fa20ad
https://doi.org/10.1101/2022.02.28.482307

Complex-dependent histone acetyltransferase activity of KAT8 determines its role in transcription and cellular homeostasis

Autor: Andrea L. Johnstone, Helene Damhofer, Ole N. Jensen, Michael-Christopher Keogh, Aliaksandra Radzisheuskaya, Yingqian Zhan, Richard Koche, Vasily V. Grinev, Daria Shlyueva, Vladimir Gorshkov, Ronald C. Hendrickson, Kristian Helin, Sergey Kovalchuk, Keli L. Rodriguez, Pavel V. Shliaha

Publikováno v: Radzisheuskaya, A, Shliaha, P V, Grinev, V V, Shlyueva, D, Damhofer, H, Koche, R, Gorshkov, V, Kovalchuk, S, Zhan, Y, Rodriguez, K L, Johnstone, A L, Keogh, M-C, Hendrickson, R C, Jensen, O N & Helin, K 2021, ' Complex-dependent histone acetyltransferase activity of KAT8 determines its role in transcription and cellular homeostasis ', Molecular Cell, vol. 81, no. 8, pp. 1749-1765.e8 . https://doi.org/10.1016/j.molcel.2021.02.012
Radzisheuskaya, A, Shliaha, P V, Grinev, V V, Shlyueva, D, Damhofer, H, Koche, R, Gorshkov, V, Kovalchuk, S, Zhan, Y, Rodriguez, K L, Johnstone, A L, Keogh, M C, Hendrickson, R C, Jensen, O N & Helin, K 2021, ' Complex-dependent histone acetyltransferase activity of KAT8 determines its role in transcription and cellular homeostasis ', Molecular Cell, vol. 81, no. 8, pp. 1749-1765.e8 . https://doi.org/10.1016/j.molcel.2021.02.012
Mol Cell

Summary Acetylation of lysine 16 on histone H4 (H4K16ac) is catalyzed by histone acetyltransferase KAT8 and can prevent chromatin compaction in vitro. Although extensively studied in Drosophila, the functions of H4K16ac and two KAT8-containing protei

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6dbe3ef6ffe7afe53ff13473b14698e7
https://portal.findresearcher.sdu.dk/da/publications/3c30878e-5994-484c-a75b-48f2827b2915

Chromatin Immunoprecipitation (ChIP) to Study DNA–Protein Interactions

Autor: Keli L. Rodriguez, Danielle Maryanski, Andrea L. Johnstone, Michael-Christopher Keogh, Eliza C. Small, Kevin J. Harvey

Publikováno v: Methods in Molecular Biology ISBN: 9781071611852

Chromatin immunoprecipitation (ChIP) is a method used to examine the genomic localization of a target of interest (e.g., proteins, protein posttranslational modifications, or DNA elements). As ChIP provides a snapshot of in vivo DNA-protein interacti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::1f33df8feb6a0ebb1a7c3a420f2fc156
https://doi.org/10.1007/978-1-0716-1186-9_20

Dysregulation of the histone demethylase KDM6B in alcohol dependence is associated with epigenetic regulation of inflammatory signaling pathways

Autor: Esi Domi, Samara Vilca, Christopher A. Rienas, Bohdan B. Khomtchouk, Zane Zeier, Danielle Maryanski, Nadja S. Andrade, Jenica D. Tapocik, Markus Heilig, Keli L. Rodriguez, Derek J. Van Booven, Estelle Barbier, Andrea L. Johnstone, Rustam Esanov, Claes Wahlestedt, Michael-Christopher Keogh, Marcus W. Meinhardt, Kenneth Lowe, Wolfgang H. Sommer

Publikováno v: Addiction Biology

Epigenetic enzymes oversee long‐term changes in gene expression by integrating genetic and environmental cues. While there are hundreds of enzymes that control histone and DNA modifications, their potential roles in substance abuse and alcohol depe

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c7bf89bfc1b565ced696f04da39fc645
https://pubmed.ncbi.nlm.nih.gov/31373129

Characterization of the plant homeodomain (PHD) reader family for their histone tail interactions

Autor: Kanishk Jain, Caroline S. Fraser, Matthew R. Marunde, Madison M. Parker, Cari Sagum, Jonathan M. Burg, Nathan Hall, Irina K. Popova, Keli L. Rodriguez, Anup Vaidya, Krzysztof Krajewski, Michael-Christopher Keogh, Mark T. Bedford, Brian D. Strahl

Publikováno v: Epigenetics & Chromatin, Vol 13, Iss 1, Pp 1-11 (2020)

Abstract Background Plant homeodomain (PHD) fingers are central “readers” of histone post-translational modifications (PTMs) with > 100 PHD finger-containing proteins encoded by the human genome. Many of the PHDs studied to date bind to unmodifie

Externí odkaz: https://doaj.org/article/42e1fad0bbb845c0b5d819ca89ab4149