The TMEM16A blockers benzbromarone and MONNA cause intracellular Ca2+-release in mouse bronchial smooth muscle cells

Autor: Ritu Dwivedi, Bernard T. Drumm, Tuleen Alkawadri, S. Lorraine Martin, Gerard P. Sergeant, Mark A. Hollywood, Keith D. Thornbury

Publikováno v: Dwivedi, R, Drumm, B T, Alkawadri, T, Martin, S L, Sergeant, G P, Hollywood, M A & Thornbury, K D 2023, ' The TMEM16A blockers benzbromarone and MONNA cause intracellular Ca 2+-release in mouse bronchial smooth muscle cells ', European Journal of Pharmacology, vol. 947, 175677 . https://doi.org/10.1016/j.ejphar.2023.175677

We investigated effects of TMEM16A blockers benzbromarone, MONNA, CaCCinhA01 and Ani9 on isometric contractions in mouse bronchial rings and on intracellular calcium in isolated bronchial myocytes. Separate concentrations of carbachol (0.1–10 μM)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::970f8b29221c6dede4fba8316c3995f1
https://pure.qub.ac.uk/en/publications/09aae45e-017b-43c4-8e65-676a736699ee

Oxidation modulates LINGO2-induced inactivation of large conductance, Ca2+-activated potassium channels

Autor: Srikanth Dudem, Pei Xin Boon, Nicholas Mullins, Heather McClafferty, Michael J. Shipston, Richard D.A. Wilkinson, Ian Lobb, Gerard P. Sergeant, Keith D. Thornbury, Irina G. Tikhonova, Mark A. Hollywood

Publikováno v: Dudem, S, Boon, P X, Mullins, N, McClafferty, H, Shipston, M J, Wilkinson, R D A, Lobb, I, Sergeant, G P, Thornbury, K D, Tikhonova, I G & Hollywood, M A 2023, ' Oxidation modulates LINGO2-induced inactivation of large conductance, Ca2 +-activated potassium channels ', The Journal of Biological Chemistry, vol. 299, no. 3, 102975 . https://doi.org/10.1016/j.jbc.2023.102975

Ca 2+ and voltage-activated K + (BK) channels are ubiquitous ion channels that can be modulated by accessory proteins, including β, γ, and LINGO1 BK subunits. In this study, we utilized a combination of site-directed mutagenesis, patch clamp electr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1495f97e0c80f1b57baf1814ccbfd671
https://pure.qub.ac.uk/en/publications/5d33d0e5-f1db-494a-a1d1-cd824ca00826

Ca

Autor: Xin Rui, Lim, Bernard T, Drumm, Gerard P, Sergeant, Mark A, Hollywood, Keith D, Thornbury

Publikováno v: Physiological reportsREFERENCES. 10(22)

Penile detumescence is maintained by tonic contraction of corpus cavernosum smooth muscle cells (CCSMC), but the underlying mechanisms have not been fully elucidated. The purpose of this study was to characterize the mechanisms underlying activation

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::aebbd26c1dfb83cd61c590109a470128
https://pubmed.ncbi.nlm.nih.gov/36394209

Functional expression of Na

Autor: Ruth M, Matthews, Eamonn, Bradley, Caoimhin S, Griffin, Xin Rui, Lim, Nicolas D, Mullins, Mark A, Hollywood, Fionnuala T, Lundy, Lorcan P, McGarvey, Gerard P, Sergeant, Keith D, Thornbury

Publikováno v: American journal of physiology. Cell physiology. 323(3)

Isolated smooth muscle cells (SMCs) from mouse bronchus were studied using the whole cell patch-clamp technique at ∼21°C. Stepping from -100 mV to -20 mV evoked inward currents of mean amplitude -275 pA. These inactivated (tau = 1.1 ms) and were a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::d42f60eb3033a516e07fc0bc79596a71
https://pubmed.ncbi.nlm.nih.gov/35876287

Non-typeable Haemophilus influenzae chronic colonization in chronic obstructive pulmonary disease (COPD)

Autor: Gordon Ramage, Lorcan McGarvey, Stephen A Carson, Bryn Short, Anna-Claire Devlin, Keith D. Thornbury, Fionnuala Lundy, James Reihill, Craig Williams, William G. Mackay, S. Lorraine Martin, Anne Crilly

Publikováno v: Short, B, Carson, S, Devlin, A-C, Reihill, J A, Crilly, A, MacKay, W, Ramage, G, Williams, C, Lundy, F T, McGarvey, L P, Thornbury, K D & Martin, L 2021, ' Non-typeable Haemophilus influenzae chronic colonization in chronic obstructive pulmonary disease (COPD) ', Critical Reviews in Microbiology, vol. 47, no. 2, pp. 192-205 . https://doi.org/10.1080/1040841X.2020.1863330

Haemophilus influenzae is the most common cause of bacterial infection in the lungs of chronic obstructive pulmonary disease (COPD) patients and contributes to episodes of acute exacerbation which are associated with increased hospitalization and mor

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f119a7a145cf6dd72ffc805044bd6f02
https://doi.org/10.1080/1040841x.2020.1863330