Zobrazeno 1 - 10
of 21
pro vyhledávání: '"Keith C. Backman"'
Publikováno v:
Journal of Bacteriology. 173:5047-5053
We have cloned and sequenced the gene for DNA ligase from Thermus thermophilus. A comparison of this sequence and those of other ligases reveals significant homology only with that of Escherichia coli. The overall amino acid composition of the thermo
Autor:
V. Dipasquantonio, Randolph T. Hatch, Rudd Edwin A, K. Venkatasubramanian, Mary Jane O'connor, Aiko Maruya, M. Radjai, Diane L. McKay, Ramaswamy Balakrishnan, Diane Shoda, Keith C. Backman
Publikováno v:
Annals of the New York Academy of Sciences. 589:16-24
Autor:
George H. Shimer, Keith C. Backman
Publikováno v:
Nonradioactive Analysis of Biomolecules ISBN: 9783540646013
Ligase chain reaction (LCR), employing just oligonucleotide probes and Principle and DNA ligase, is capable of detecting approximately
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1c7f0dc2ca4ef816e3fb2e163e251c39
https://doi.org/10.1007/978-3-642-57206-7_32
https://doi.org/10.1007/978-3-642-57206-7_32
Publikováno v:
Gene. 138(1-2)
A cassette of genes from bacteriophage X, when carried on a derivative of bacteriophage Mu, renders strains of Escherichia coli (and in principle other Mu-sensitive bacteria) capable of supporting X-based expression vectors, such as rearrangement vec
Autor:
Douglas P. Malinowski, Michael C. Little, James G. Nadeau, Rüdiger Rüger, Amy Alexander, Melinda S. Fraiser, David Segev, Euan K. James, Erko Stackebrandt, Werner Liesack, G. Terrance Walker, Keith C. Backman, George H. ShimerJr., James L. Schram
Publikováno v:
Nonradioactive Labeling and Detection of Biomolecules ISBN: 9783662001462
The polymerase chain reaction (PCR) represents the most common and widespread method for the direct amplification of specific sequences of nucleic acid target molecules. The basic reaction is comprised of three steps: 1. Denaturation of the target DN
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::731183cfee5f3c434fd125ab479d91af
https://doi.org/10.1007/978-3-662-00144-8_16
https://doi.org/10.1007/978-3-662-00144-8_16
Autor:
Keith C. Backman
Publikováno v:
Science. 314:419-419
The controversy engendered by string theory (“A ‘landscape’ too far?”, T. Siegfried, News Focus, 11 Aug., p. [750][1]) illustrates the dangers of assuming that mathematical models that seem to account for our real universe are in fact substan
Autor:
Mark Ptashne, Barbara J. Meyer, Robert T. Sauer, Andrea Jeffrey, Keith C. Backman, MZ Humayun, Russell Maurer
Publikováno v:
Science. 194:156-161
Autor:
Herbert W. Boyer, Keith C. Backman
Publikováno v:
Gene. 26:197-203
Only one polypeptide specified by plasmid pBR322 is necessary to determine tetracycline resistance. Small deletions in pBR322 constructed in vitro which result in the lack of ability to confer tetracycline resistance in vivo also result in the absenc
Publikováno v:
Proceedings of the National Academy of Sciences. 78:3743-3747
We have cloned and characterized a fragment of the Escherichia coli chromosome spanning glnA, the structural gene for glutamine synthetase (L-glutamate:ammonia ligase (ADP-forming), EC 6.3.1.2]. The fragment also carriers glnG, whose product is neces
Autor:
Anthony R. Poteete, Thomas M. Roberts, Stephen L. Swanberg, Gerard E. Riedel, Keith C. Backman
Publikováno v:
Gene. 12:123-127
We describe a plasmid cloning vehicle, pTR262, which allows a strong positive selection (resistance to tetracycline) for transformants bearing plasmids which have DNA insertions. pTR262 is derived from plasmid pBR322 and contains the cI gene and adja