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pro vyhledávání: '"Keith A. Knisley"'
Autor:
Keith A. Knisley, L. Scott Rodkey
This chapter explores the effects of alternative buffer formulations on resolution of separated cells in the McDonnell-Douglas continuous flow electrophoresis system. Specifically, organic zwitterionic buffers were selected for analysis of their util
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::861fa5bdbaeea4d9012dd9f049619ea8
https://doi.org/10.1201/9781003069188-10
https://doi.org/10.1201/9781003069188-10
Autor:
Harry M. Weitlauf, Janelle Dorsett, Keith A. Knisley, Vaughan H. Lee, Brooke Phillips, Kayla D. Weitlauf
Publikováno v:
Biology of Reproduction. 55:548-558
Two beta-galactoside-binding proteins were isolated from uteroplacental complexes of pregnant mice and identified as the S-Lac lectins galectin-1 and galectin-3. The spatiotemporal pattern of appearance of those proteins was determined by immunocytoc
Autor:
L. Scott Rodkey, Keith A. Knisley
Publikováno v:
Electrophoresis. 13:220-224
A protocol is described for monitoring the heterogeneity of end products of organic syntheses yielding amphoteric molecules containing two or more amino groups. This protocol was found to be a valuable aid in synthesis of carrier ampholytes for speci
Publikováno v:
Developmental and comparative immunology. 17(5)
Neonatal germfree (GF) colostrum-deprived and conventional (CV) colostrum-fed piglets were immunized IP with p -azo-phenylarsonate-bovine gamma globulin (Ars-BGG) in Freund's adjuvant to study the development of the immune response in the absence or
Autor:
Harry M. Weitlauf, Keith A. Knisley
Publikováno v:
Biology of reproduction. 46(5)
Changes in the expression of specific cell surface antigens on preimplantation mouse embryos were examined by immunocytochemistry. Embryos were recovered at various times during the preimplantation phase of normal pregnancy, and from pregnancies with
Autor:
Keith A. Knisley, Rodkey Ls
Publikováno v:
Electrophoresis. 11(11)
Continous flow zone electrophoresis (CFE) and recycling isoelectric focusing (RIEF) are two of the alternative formats for fluid phase preparative isolation of biological products in liquid separation media. The McDonnell Douglas CFE system has been
Autor:
Keith A. Knisley, L. Scott Rodkey
Publikováno v:
Journal of Immunological Methods. 95:79-87
A sensitive and specific method is proposed for the analysis of specific antibody clonotype changes occurring during an immune response and for comparing multiple sera for antibody clonotype similarities. Polyclonal serum antibodies separated by isoe
Autor:
L. Scott Rodkey, Keith A. Knisley
Publikováno v:
Electrophoresis. 9(4)
Serum was collected from rabbits at 2-day intervals following a single injection with tetanus toxoid or at weekly intervals following multiple injections with Micrococcus lysodeikticus cell walls. These sera were analyzed for the presence of individu
Autor:
L. D. Pearson, Keith A. Knisley
Publikováno v:
Veterinary immunology and immunopathology. 16(1-2)
Conditions for the production and assay of ovine T cell growth factor (TCGF) activity were evaluated. Peripheral blood leukocytes were stimulated with concanavalin A (Con A) in the presence of 2% autologous serum or serum-free media. Supernatants wer