Zobrazeno 1 - 10
of 205
pro vyhledávání: '"Keitaro Hiromi"'
Publikováno v:
International Journal of Peptide and Protein Research. 10:219-225
The kinetic study of fluorescence stopped-flow method suggested that the interaction between lipoxygenase and H2O2 is consistent with a simple irreversible one-step mechanism. The activation energy of the reaction was 7.2 kcal/mol. Participation of a
Autor:
Tsutomu Morikawa, Junzo Hirose, Hirokuni Sakuragi, Mituru Kikkawa, Keitaro Hiromi, Masayoshi Minakami, Hiroyuki Iwamoto, Kaori Inoue
Publikováno v:
Inorganica Chimica Acta. 273:204-212
Bilirubin oxidase obtained from Trachyderma tsunodae K-2593 is a multi-copper enzyme which has type 1:type 2:type 3 copper atoms in the ratio 1:1:2. Anion binding properties of bilirubin oxidase were investigated to determine the structural and funct
Autor:
Takanori Muto, Satoshi Hashimoto, Keitaro Hiromi, Ben'ichiro Tonomura, Tokuo Sukata, Naofumi Shimizu, Kuniyo Inouye, Yuji Ohkubo, Teisuke Takita
Publikováno v:
Journal of Biochemistry. 121:244-250
The formation of an enzyme.lysyladenylate complex was studied with a highly purified lysyl-tRNA synthetase [L-lysine:tRNALYS ligase (AMP-forming); EC 6.1.1.6] from Bacillus stearothermophilus. The apparent dissociation equilibrium constants of the en
Publikováno v:
Carbohydrate Research. 282:171-180
The subsite structure of bacterial saccharifying alpha-amylase (BSAm) was elucidated by two methods using a series of maltooligosaccharides labeled with [14C]D-glucose at the reducing end. The rate parameter k0/Km and the cleavage frequency were obta
Autor:
Masahiro Ohno, Masayuki Ohmori, Akiyoshi Tanaka, Hiroyuki Iwamoto, Shuzo Sakai, Keitaro Hiromi, Junzo Hirose
Publikováno v:
The Journal of Biochemistry. 116:1264-1268
The change in fluorescence spectra of crystalline pullulanase from Klebsiella pneumoniae caused by the addition of alpha-, beta-, and gamma-cyclodextrins and 6-O-alpha-glucosyl-alpha-cyclodextrin and 6-O-alpha-glucosyl-beta-cyclodextrin was investiga
Autor:
Keiko Masuda-Momma, Ben'ichiro Tonomura, Kin-ichiro Miura, Kuniyo Inouye, Toyohiro Shimakawa, Keitaro Hiromi, Izumi Kumagai, Shuichi Kojima
Publikováno v:
The Journal of Biochemistry. 114:906-911
An ultraviolet absorption difference spectrum characteristic of the ionization change of a tyrosyl residue was observed on the binding of subtilisin BPN' with Streptomyces subtilisin inhibitor (SSI) at alkaline pH. This difference spectrum was consid
Autor:
Masayuki Ohmori, Hitoshi Hashimoto, Junzo Hirose, Masahiro Ohno, Keitaro Hiromi, Hiroyuki Iwamoto, Harumi Fukada, Katsutada Takahashi, Shuzo Sakai
Publikováno v:
The Journal of Biochemistry. 113:93-96
The interaction between pullulanase from Klebsiella pneumoniae and alpha-, beta-, and gamma-cyclodextrins and 6-O-alpha-glucosyl-alpha-cyclodextrin and 6-O-alpha-glucosyl-beta-cyclodextrin was examined by means of inhibition studies of the enzyme act
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 57:1902-1905
alpha-Glucosidase was partially purified 103-fold from a cell-free extract of Torulaspora pretoriensis YK-1 by column chromatography on Toyopearl HW55F, DEAE-Toyopearl 650M, hydroxylapatite and phenyl-Toyopearl 650M. Further purification by preparati
Autor:
Keitaro Hiromi
Publikováno v:
Journal of the agricultural chemical society of Japan. 66:1319-1326
Autor:
Masatake Ohnishi, Yokox Fujioka, Chieko Hashizume, Shigeo Takewuti, Ben'ichiro Tonomura, Keitaro Hiromi, Takahito Yoshida
Publikováno v:
Journal of the Japanese Society of Starch Science. 38:41-44
Binding of a substrate xylose to Streptomyces xylose isomerase (XIase) was observed by using a change (decrease) in fluorescence intensity (based on tryptophan residue) as a probe, and the binding parameters Kb and ΔFmax were evaluated for the xylos