Residue-specific radical-driven dissociation of peptides using matrix-assisted laser desorption/ionization in-source decay with hydrogen-donating and -abstracting matrices

Autor: Keishiro Nagoshi, Mitsuo Takayama

Publikováno v: International Journal of Mass Spectrometry. 436:25-32

Radical-driven dissociation (RDD) of peptides was examined by MALDI-ISD with hydrogen-donating and -abstracting matrices. The observed fragment ions could be explained by the RDD of carbonyl-carbon (CCO)-centered hydrogen-excess radical ions [M + HCO

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::44263a0e76cc8f0bfa28928b15bf6993
https://doi.org/10.1016/j.ijms.2018.11.002

Access of Hydrogen-Radicals to the Peptide-Backbone as a Measure for Estimating the Flexibility of Proteins Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

Autor: Kazuma Inatomi, Mitsuo Takayama, Ryunosuke Iimuro, Keishiro Nagoshi

Publikováno v: International Journal of Molecular Sciences, Vol 15, Iss 5, Pp 8428-8442 (2014)
International Journal of Molecular Sciences
Volume 15
Issue 5
Pages 8428-8442

A factor for estimating the flexibility of proteins is described that uses a cleavage method of “in-source decay (ISD)” coupled with matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). The MALDI-ISD spectra of bovine serum a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8553e071fb28dda558a8d2ca73204c4c
http://www.mdpi.com/1422-0067/15/5/8428

Laser Desorption Ionization Mass Spectrometry by Using Surface Plasmon Excitation on Gold Nanoparticle

Autor: Takashi Korenaga, Kohei Shibamoto, Keishiro Nagoshi, Kazuhiro Sakata

Publikováno v: The Journal of Physical Chemistry C. 113:17774-17779

We show development of a laser desorption/ionization (LDI) method with ultrahigh sensitivity by adding gold nanoparticles with a diameter of several tens of nanometers into a sample solution. In this paper, we succeeded in detection of an ultratrace

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::7000cbf62ccaefe75c04ceb79e527cb7
https://doi.org/10.1021/jp9020432

Timeframe Dependent Fragment Ions Observed in In-Source Decay Experiments with β-Casein Using MALDI MS

Autor: Mitsuo Takayama, Shinichi Iwamoto, Keishiro Nagoshi, Koichi Tanaka, Sadanori Sekiya

Publikováno v: Journal of the American Society for Mass Spectrometry. 26(9)

The fragment ions observed with time-of-flight (TOF) and quadrupole ion trap (QIT) TOF mass spectrometers (MS) combined with matrix-assisted laser desorption/ionization in-source decay (MALDI-ISD) experiments of phosphorylated analytes β-casein and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4a09982ef22bba5894756c73418536b9
https://pubmed.ncbi.nlm.nih.gov/26148524