Zobrazeno 1 - 10
of 18
pro vyhledávání: '"Keiko Umezu"'
Autor:
Michio Hayashi, Keiko Umezu
Publikováno v:
Genes & Genetic Systems. 92:73-80
We have been studying the genetic events, including chromosome loss, chromosome rearrangements and intragenic point mutations, that are responsible for the deletion of a URA3 marker in a loss of heterozygosity (LOH) assay in the yeast Saccharomycess
Publikováno v:
FEBS Journal. 275:5733-5744
Recently, we have shown that phenyl hydroquinone, a hepatic metabolite of the Ames test-negative carcinogen o-phenylphenol, efficiently induced aneuploidy in Saccharomyces cerevisiae. We further found that phenyl hydroquinone arrested the cell cycle
Publikováno v:
Genetics. 164:31-46
In previous studies of the loss of heterozygosity (LOH), we analyzed a hemizygous URA3 marker on chromosome III in S. cerevisiae and showed that homologous recombination is involved in processes that lead to LOH in multiple ways, including allelic re
Publikováno v:
Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 504:157-172
The SGS1 gene of Saccharomyces cerevisiae is a member of the RecQ helicase family, which includes the human BLM, WRN and RECQL4 genes responsible for Bloom and Werner's syndrome and Rothmund-Thomson syndrome, respectively. Cells defective in any of t
Publikováno v:
Genetics. 160:97-110
The structural analysis of aberrant chromosomes is important for our understanding of the molecular mechanisms underlying chromosomal rearrangements. We have identified a number of diploid Saccharomyces cerevisiae clones that have undergone loss of h
Publikováno v:
Molecular Cell. 2(1):9-22
Three temperature-sensitive S. cerevisiae RFA1 alleles were found to cause elevated mutation rates. These mutator phenotypes resulted from the accumulation of base substitutions, frameshifts, gross deletions (8 bp-18 kb), and nonreciprocal translocat
Publikováno v:
Genetics. 148:989-1005
Replication protein A (RPA) is a single-stranded DNA-binding protein identified as an essential factor for SV40 DNA replication in vitro. To understand the in vivo functions of RPA, we mutagenized the Saccharomyces cerevisiae RFA1 gene and identified
Autor:
Keiko Umezu, Richard D. Kolodner
Publikováno v:
Journal of Biological Chemistry. 269:30005-30013
RecA promotes homologous pairing of single-stranded DNA (ssDNA) with double-stranded DNA (dsDNA). This reaction occurs inefficiently if the ssDNA substrate is preincubated with Escherichia coli ssDNA-binding protein (SSB). However, RecO and RecR can
Publikováno v:
Proceedings of the National Academy of Sciences. 90:3875-3879
The Escherichia coli RecF, RecO, and RecR proteins were analyzed for their effect on RecA-mediated pairing of single-stranded circular DNA and homologous linear duplex DNA substrates. As shown by other workers, joint molecule formation by RecA was in
Autor:
Hiroaki Nakayama, Keiko Umezu
Publikováno v:
Journal of Molecular Biology. 230:1145-1150
RecQ protein of Escherichia coli is a DNA helicase implicated in the RecF pathway of genetic recombination. To gain insight into the mode of its action, the effect of single-stranded DNA-binding proteins (SSBs) on the RecQ-mediated unwinding reaction