Zobrazeno 1 - 10
of 15
pro vyhledávání: '"Keiko Tamura-Kawakami"'
Autor:
Emiko Mihara, Hidenori Hirai, Hideki Yamamoto, Keiko Tamura-Kawakami, Mami Matano, Akira Kikuchi, Toshiro Sato, Junichi Takagi
Publikováno v:
eLife, Vol 5 (2016)
Wnt plays important role during development and in various diseases. Because Wnts are lipidated and highly hydrophobic, they can only be purified in the presence of detergents, limiting their use in various in vitro and in vivo assays. We purified N-
Externí odkaz:
https://doaj.org/article/9b04a5fa9a914e4e98f0739e29aaa5bb
Publikováno v:
The Journal of Biochemistry. 167:577-586
Activation of a tyrosine kinase receptor Met by hepatocyte growth factor (HGF) requires binding of proteolytically activated, two-chain (tc) HGF, but the biochemical detail of this ligand–receptor interaction specificity remains elusive because bio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ed3784523ffcb5a6345aa0d0a69b5bc1
https://doi.org/10.1093/jb/mvaa006
https://doi.org/10.1093/jb/mvaa006
Autor:
Junichi Takagi, Mika K. Kaneko, Emiko Mihara, Katsu Takahashi, Yuki Fujii, Yu Kitago, Yukinari Kato, Keiko Tamura-Kawakami, Naoko Norioka, Sanae Tabata
Publikováno v:
Protein Expression and Purification. 147:94-99
Peptide-based affinity tags are commonly used in recombinant production/purification of proteins, and are often preceded or followed by a protease recognition sequence to allow tag removal. We describe a rat monoclonal antibody 2H5 recognizing an und
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::57409d42b8e5e0312d96e005b7187767
https://doi.org/10.1016/j.pep.2018.03.004
https://doi.org/10.1016/j.pep.2018.03.004
Autor:
Junichi Takagi, Naoyuki Miyazaki, Keiko Tamura-Kawakami, Hidenori Hirai, Shintaro Maeda, Kyoko Matoba, Emiko Mihara, Samuel Thompson, Kenji Iwasaki
Publikováno v:
Cell Reports. 18(1):32-40
LDL-receptor-related protein 6 (LRP6) is a single-pass membrane glycoprotein with a large modular ectodomain and forms a higher order signaling platform upon binding Wnt ligands on the cell surface. Although multiple crystal structures are available
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6ef294a05a0874bf8e88e049dae5897b
Autor:
Satoshi Ogasawara, Yukinari Kato, Keiko Tamura-Kawakami, Masataka Umitsu, Mika K. Kaneko, Kunio Matsumoto, Katsuya Sakai, Junichi Takagi, Ryoko Asaki
Publikováno v:
Scientific Reports
HGF-Met signaling contributes to various biological events by controlling cell migration. Since the abnormal activation of Met receptor causes cancer progression, inhibitors such as neutralizing antibodies are regarded as promising therapeutics. HGF
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fb85949667a6c16b56088d675bb9b8f9
https://doi.org/10.1038/srep33149
https://doi.org/10.1038/srep33149
Autor:
Hideaki Unno, Junichi Takagi, Emiko Mihara, Keiko Tamura-Kawakami, Maiko Nampo, Norihisa Yasui, Kyoichi Matsumoto
Publikováno v:
Journal of Immunological Methods. 352:153-160
LRP6 is a cell surface molecule that plays a critical role in the Wnt signaling pathway, and is implicated in numerous human diseases. Studies of cellular signaling mediated by LRP6 have relied on overexpression experiments, due to the lack of good m
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::21058995be59d9d0e5b9e36ef6420373
https://doi.org/10.1016/j.jim.2009.11.016
https://doi.org/10.1016/j.jim.2009.11.016
Autor:
Mitsuharu Hattori, Takeshi Sangawa, Masamichi Nagae, Sanae Tabata, Keiko Tamura-Kawakami, Junichi Takagi, Ayako Beppu, Norihisa Yasui, Terukazu Nogi
Publikováno v:
Protein Science. 17:2120-2126
Biologically important human proteins often require mammalian cell expression for structural studies, presenting technical and economical problems in the production/purification processes. We introduce a novel affinity peptide tagging system that use
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b42e66c80ade857c6bf26eccabac771e
https://doi.org/10.1110/ps.038299.108
https://doi.org/10.1110/ps.038299.108
Autor:
Yuki Fujii, Masataka Umitsu, Ryoko Asaki, Junichi Takagi, Keiko Tamura-Kawakami, Yu Kitago, Takao Arimori
Publikováno v:
Structure. 25:1611-1622.e4
Summary Antibody fragments are frequently used as a "crystallization chaperone" to aid structural analysis of complex macromolecules that are otherwise crystallization resistant, but conventional fragment formats have not been designed for this parti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::01a21b8941519090e094dfcdee11a732
https://doi.org/10.1016/j.str.2017.08.011
https://doi.org/10.1016/j.str.2017.08.011
Publikováno v:
Journal of proteomics. 73(9)
Recombinant production of extracellular glycoproteins in stable mammalian cell lines is an ideal technique for obtaining a large quantity of high-quality proteins. In most cases, however, current methodologies do not allow for sufficiently rapid cell
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9626f435b25af23a9f1e1cc0bfea2475
https://pubmed.ncbi.nlm.nih.gov/20566373
https://pubmed.ncbi.nlm.nih.gov/20566373