Zobrazeno 1 - 10
of 405
pro vyhledávání: '"Kei K. Ito"'
Publikováno v:
iScience, Vol 27, Iss 3, Pp 109309- (2024)
Summary: Experimental analysis of functionally related genes is key to understanding biological phenomena. The selection of genes to study is a crucial and challenging step, as it requires extensive knowledge of the literature and diverse biomedical
Externí odkaz:
https://doaj.org/article/204905eb1fde4cc88d27fa245d64e707
Autor:
Akira Mabuchi, Shoji Hata, Mariya Genova, Chiharu Tei, Kei K. Ito, Masayasu Hirota, Takuma Komori, Masamitsu Fukuyama, Takumi Chinen, Atsushi Toyoda, Daiju Kitagawa
Publikováno v:
BMC Genomics, Vol 24, Iss 1, Pp 1-16 (2023)
Abstract Background Recent advances in CRISPR technology have enabled us to perform gene knock-in in various species and cell lines. CRISPR-mediated knock-in requires donor DNA which serves as a template for homology-directed repair (HDR). For knock-
Externí odkaz:
https://doaj.org/article/ee096e6001b749df988891c536a3bf4d
Publikováno v:
Non-Coding RNA, Vol 6, Iss 1, p 13 (2020)
Mounting experimental evidence shows that non-coding RNAs (ncRNAs) serve a wide variety of biological functions. Recent studies suggest that a part of ncRNAs are critically important for supporting the structure of subcellular architectures. Here, we
Externí odkaz:
https://doaj.org/article/c9e375e42ab04d4e865bfe50a6b6ef04
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-13 (2019)
Centriole disengagement occurs towards mitotic exit and involves cleavage of pericentrin, a component of the pericentriolar material. Here the authors show that depletion of the centrosomal protein Cep57 leads to precocious centriole disengagement, a
Externí odkaz:
https://doaj.org/article/759774e53a804a65b6bc1ac46668a329
Autor:
Akira Mabuchi, Shoji Hata, Mariya Genova, Chiharu Tei, Kei K Ito, Masayasu Hirota, Takuma Komori, Masamitsu Fukuyama, Takumi Chinen, Atsushi Toyoda, Daiju Kitagawa
Recent advances in CRISPR technology have enabled us to perform gene knock-in in various species and cell lines. CRISPR-mediated knock-in requires donor DNA which serves as a template for homology-directed repair (HDR). For knock-in of short sequence
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f905236a6edcb7ef758d6db845df0e7a
https://doi.org/10.1101/2022.06.01.494308
https://doi.org/10.1101/2022.06.01.494308
Publikováno v:
SSRN Electronic Journal.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::865cefb0770edd1b98e3433c71fee092
https://doi.org/10.2139/ssrn.4290058
https://doi.org/10.2139/ssrn.4290058
MotivationIn cellular biology, researchers design wet experiments by reading the relevant articles and considering the described experiments and results. Today, researchers spend a long time exploring the literature in order to plan experiments.Resul
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c291daaf3c62ab5a89ef7d629e56ddc4
https://doi.org/10.1101/2021.12.05.471323
https://doi.org/10.1101/2021.12.05.471323
Autor:
Shoji Hata, Daiju Kitagawa, Koki Watanabe, Kyohei Matsuhashi, Takumi Chinen, Haruki Ishida, Kei K. Ito
Publikováno v:
The Journal of Cell Biology
Ito et al. revealed that centrosomal proteins Cep57 and Cep57L1 cooperatively maintain centriole engagement during interphase in human cells. Codepletion of Cep57 and Cep57L1 induces precocious centriole disengagement in interphase, which results in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bfe82adf1f9d86a22f9ed77b9c768132
https://doi.org/10.1083/jcb.202005153
https://doi.org/10.1083/jcb.202005153
Autor:
Takashi Fujii, Miki Wada, Jun-Song Wang, Yayoi Nomura, Andrés D. Maturana, Tsukasa Kusakizako, Yurika Yamada, So Iwata, Osamu Nureki, Yoshiko Nakada-Nakura, Atsuhiro Tomita, Motoyuki Hattori, Jie Ma, Keiichi Namba, Kei K. Ito, Minxuan Sun, H. Takeda, Fei Jin, Tomoko Uemura, Shichen Su, Norimichi Nomura, Ye Yu, Kehong Liu
MgtE is a Mg2+ channel conserved in organisms ranging from prokaryotes to eukaryotes, including humans, and plays an important role in Mg2+ homeostasis. The previously determined MgtE structures in the Mg2+-bound, closed state and structure-based fun
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::cee246f6a6872896253039229e7be2e3
https://doi.org/10.1101/2020.08.27.270991
https://doi.org/10.1101/2020.08.27.270991
Autor:
Koki Watanabe, Kyohei Matsuhashi, Takumi Chinen, Daiju Kitagawa, Grant S. Stewart, Shoji Hata, Kei K. Ito
Microcephalic primordial dwarfism (MPD) is a genetic disorder characterized by short stature and microcephaly. MPD-related genes are known to regulate centrosome biogenesis, DNA replication or the DNA damage response. Although some of the MPD-related
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aa176ef1766d743c466a069998229490