Single-cell transcriptome and translatome dual-omics reveals potential mechanisms of human oocyte maturation

Autor: Wenqi Hu, Haitao Zeng, Yanan Shi, Chuanchuan Zhou, Jiana Huang, Lei Jia, Siqi Xu, Xiaoyu Feng, Yanyan Zeng, Tuanlin Xiong, Wenze Huang, Peng Sun, Yajie Chang, Tingting Li, Cong Fang, Keliang Wu, Lingbo Cai, Wuhua Ni, Yan Li, Zhiyong Yang, Qiangfeng Cliff Zhang, RiCheng Chian, Zijiang Chen, Xiaoyan Liang, Kehkooi Kee

Publikováno v: Nature Communications, Vol 13, Iss 1, Pp 1-17 (2022)

Development of methods for simultaneous single cell analysis of transcription and translation is still underway. Here, Hu et al. develop single-cell transcriptome and translatome dual-omics on human oocytes, which enables them to identify OOSP2 as an

Externí odkaz: https://doaj.org/article/6ca410494d72486ebe97c1923beedc55

Single-cell profiling of lncRNAs in human germ cells and molecular analysis reveals transcriptional regulation of LNC1845 on LHX8

Autor: Nan Wang, Jing He, Xiaoyu Feng, Shengyou Liao, Yi Zhao, Fuchou Tang, Kehkooi Kee

Publikováno v: eLife, Vol 12 (2023)

Non-coding RNAs exert diverse functions in many cell types. In addition to transcription factors from coding genes, non-coding RNAs may also play essential roles in shaping and directing the fate of germ cells. The presence of many long non-coding RN

Externí odkaz: https://doaj.org/article/b45a46fff29f407b83c8a0275c9212fa

IGSF11 is required for pericentric heterochromatin dissociation during meiotic diplotene.

Autor: Bo Chen, Gengzhen Zhu, An Yan, Jing He, Yang Liu, Lin Li, Xuerui Yang, Chen Dong, Kehkooi Kee

Publikováno v: PLoS Genetics, Vol 17, Iss 9, p e1009778 (2021)

Meiosis initiation and progression are regulated by both germ cells and gonadal somatic cells. However, little is known about what genes or proteins connecting somatic and germ cells are required for this regulation. Our results show that deficiency

Externí odkaz: https://doaj.org/article/d38d544322054ec993d85cad0a8e0a6f

5-Formylcytosine landscapes of human preimplantation embryos at single-cell resolution.

Autor: Yun Gao, Lin Li, Peng Yuan, Fan Zhai, Yixin Ren, Liying Yan, Rong Li, Ying Lian, Xiaohui Zhu, Xinglong Wu, Kehkooi Kee, Lu Wen, Jie Qiao, Fuchou Tang

Publikováno v: PLoS Biology, Vol 18, Iss 7, p e3000799 (2020)

Epigenetic dynamics, such as DNA methylation and chromatin accessibility, have been extensively explored in human preimplantation embryos. However, the active demethylation process during this crucial period remains largely unexplored. In this study,

Externí odkaz: https://doaj.org/article/bbed9b1f4ddc46b680a960bbec4fe512

Quality criteria for in vitro human pluripotent stem cell-derived models of tissue-based cells

Autor: Francesca Pistollato, Anna Bal-Price, Sandra Coecke, Surat Parvatam, David Pamies, Katherine Czysz, Jie Hao, Kehkooi Kee, Adrian Kee Keong Teo, Shuaishuai Niu, Anja Wilmes, Lena Smirnova, Christian Freund, Christine Mummery, Glyn Stacey

Publikováno v: Pistollato, F, Bal-Price, A, Coecke, S, Parvatam, S, Pamies, D, Czysz, K, Hao, J, Kee, K, Teo, A K K, Niu, S, Wilmes, A, Smirnova, L, Freund, C, Mummery, C & Stacey, G 2022, ' Quality criteria for in vitro human pluripotent stem cell-derived models of tissue-based cells ', Reproductive Toxicology, vol. 112, pp. 36-50 . https://doi.org/10.1016/j.reprotox.2022.06.003
Reproductive Toxicology, 112, 36-50. Elsevier Inc.
Reproductive Toxicology, 112, 36-50. PERGAMON-ELSEVIER SCIENCE LTD

The advent of the technology to isolate or generate human pluripotent stem cells provided the potential to develop a wide range of human models that could enhance understanding of mechanisms underlying human development and disease. These systems are

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8146a56e62179eec18f54a0202bf68df
https://doi.org/10.1016/j.reprotox.2022.06.003