Zobrazeno 1 - 10
of 177
pro vyhledávání: '"Kees Jalink"'
Publikováno v:
iScience, Vol 27, Iss 7, Pp 110268- (2024)
Summary: The outcome of cell signaling depends not only on signal strength but also on temporal progression. We use Fluorescence Lifetime Imaging of Resonance Energy Transfer (FLIM/FRET) biosensors to investigate intracellular signaling dynamics. We
Externí odkaz:
https://doaj.org/article/31c1435066444e929e0fb686efc30850
Autor:
Miguel Vizoso, Colin E. J. Pritchard, Lorenzo Bombardelli, Bram van den Broek, Paul Krimpenfort, Roderick L. Beijersbergen, Kees Jalink, Jacco van Rheenen
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-15 (2022)
Achieving spatial control of gene expression is important. Here the authors report an optimised photoactivatable Cre recombinase system, doxycycline- and light-inducible Cre recombinase (DiLiCre), and generate a DiLiCre mouse line which they use for
Externí odkaz:
https://doaj.org/article/ca99867f51bf4b11a30935fbe673b977
Autor:
Rolf Harkes, Olga Kukk, Sravasti Mukherjee, Jeffrey Klarenbeek, Bram van den Broek, Kees Jalink
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
Abstract Fluorescence Lifetime Imaging (FLIM) is an intrinsically quantitative method to screen for protein–protein interactions and is frequently used to record the outcome of signal transduction events. With new highly sensitive and photon effici
Externí odkaz:
https://doaj.org/article/fb0e1af6ef0d4af28dabea59f40f525b
Autor:
Paul L.G. Slangen, Yaara Porat, Marjolijn Mertz, Bram van den Broek, Kees Jalink, Mark C. de Gooijer, Olaf van Tellingen, Gerben R. Borst
Publikováno v:
STAR Protocols, Vol 3, Iss 2, Pp 101246- (2022)
Summary: Tumor Treating Fields (TTFields) are an FDA-approved anticancer treatment using alternating electric fields. Here, we present a protocol to perform live-cell imaging (LCI) of cells during TTFields treatment with the Inovitro LiveTM system. T
Externí odkaz:
https://doaj.org/article/f04b5b459e334ac685b4ae8f4181bf0a
Autor:
Koen van den Dries, Leila Nahidiazar, Johan A. Slotman, Marjolein B. M. Meddens, Elvis Pandzic, Ben Joosten, Marleen Ansems, Joost Schouwstra, Anke Meijer, Raymond Steen, Mietske Wijers, Jack Fransen, Adriaan B. Houtsmuller, Paul W. Wiseman, Kees Jalink, Alessandra Cambi
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-16 (2019)
Podosomes are actin-based protrusions used by cells for invasion and local degradation but the structure underlying their protrusiveness and mechanosensitivity is unclear. Here, the authors report that podosomes have a modular actin nano-architecture
Externí odkaz:
https://doaj.org/article/f02ec8baa69243f385139e1d26c11672
Autor:
Daniela Leyton-Puig, Tadamoto Isogai, Elisabetta Argenzio, Bram van den Broek, Jeffrey Klarenbeek, Hans Janssen, Kees Jalink, Metello Innocenti
Publikováno v:
Nature Communications, Vol 8, Iss 1, Pp 1-14 (2017)
Clathrin lattices coat flat membrane regions, called plaques, whose regulation and function are poorly understood. Here the authors find that plaques are regulated by actin dynamics and contain both the endocytic and the cell adhesion machineries, an
Externí odkaz:
https://doaj.org/article/a675eefc386747468d8c562060201f7b
Autor:
Umberto Rodella, Samuele Negro, Michele Scorzeto, Elisanna Bergamin, Kees Jalink, Cesare Montecucco, Nobuhiro Yuki, Michela Rigoni
Publikováno v:
Disease Models & Mechanisms, Vol 10, Iss 5, Pp 597-603 (2017)
The neuromuscular junction is exposed to different types of insult, including mechanical trauma, toxins and autoimmune antibodies and, accordingly, has retained through evolution a remarkable ability to regenerate. Regeneration is driven by multiple
Externí odkaz:
https://doaj.org/article/495ecd184cb94f6ab74543a66733deb1
Publikováno v:
PLoS ONE, Vol 14, Iss 1, p e0209563 (2019)
The nonspecific divalent cation channel TRPM7 (transient receptor potential-melastatin-like 7) is involved in many Ca2+ and Mg2+-dependent cellular processes, including survival, proliferation and migration. TRPM7 expression predicts metastasis and r
Externí odkaz:
https://doaj.org/article/d2306007c79e4a6089aca46e52bfe946
Autor:
Femke M. Feringa, Lenno Krenning, André Koch, Jeroen van den Berg, Bram van den Broek, Kees Jalink, René H. Medema
Publikováno v:
Nature Communications, Vol 7, Iss 1, Pp 1-10 (2016)
The DNA damage response induces a reversible arrest at different cell cycle stages. Here the authors find that loss of the APC/C inhibitor Emi1 in antephase (late G2) results in hypersensitivity to DNA damage and cell cycle exit at DNA damage levels
Externí odkaz:
https://doaj.org/article/a2284338e2d945e09762ac87bdafcf59
Autor:
Daniela Leyton-Puig, Katarzyna M. Kedziora, Tadamoto Isogai, Bram van den Broek, Kees Jalink, Metello Innocenti
Publikováno v:
Biology Open, Vol 5, Iss 7, Pp 1001-1009 (2016)
Super-resolution microscopy (SRM) allows precise localization of proteins in cellular organelles and structures, including the actin cytoskeleton. Yet sample preparation protocols for SRM are rather anecdotal and still being optimized. Thus, SRM-base
Externí odkaz:
https://doaj.org/article/8f3bdf4392c8494fb004c17914ee4257