Výsledky vyhledávání - "Kazuya Shiroishi"

Identification and Characterization of the ackA (Acetate Kinase A)-pta (Phosphotransacetylase) Operon and Complementation Analysis of Acetate Utilization by an ackA-pta Deletion Mutant of Escherichia coli

Autor: Kazuya Shiroishi, Shigeyuki Ichihara, Kiyoshi Hosono, Hiroyuki Kakuda

Publikováno v: The Journal of Biochemistry. 116:916-922

The pta gene encoding phosphotransacetylase was cloned on a high copy plasmid with or without the ackA gene encoding acetate kinase in Escherichia coli. The acetate kinase and phosphotransacetylase were overproduced in cells harboring the plasmid pos

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::31991891af1ce4bc849dfdceb0fe8aca
https://doi.org/10.1093/oxfordjournals.jbchem.a124616

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Construction of Pta-Ack Pathway Deletion Mutants ofEscherichia coliand Characteristic Growth Profiles of the Mutants in a Rich Medium

Autor: Kazuya Shiroishi, Shigeyuki Ichihara, Hiroyuki Kakuda, Kiyoshi Hosono

Publikováno v: Bioscience, Biotechnology, and Biochemistry. 58:2232-2235

Escherichia coli grown in a rich medium excreted acetate and reused the acetate. Using cloned genes and a plasmid with a temperature-sensitive replication origin, three kinds of Pta-Ack pathway deletion mutants were constructed. Acetate production an

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::443cd2ff6e12683273d2d20b271ec9b0
https://doi.org/10.1271/bbb.58.2232

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