Zobrazeno 1 - 10
of 19
pro vyhledávání: '"Kazuo Hanawa"'
Autor:
Subhendu Sekhar Bag, Yoshio Saito, Kenzo Fujimoto, Isao Saito, Katsuhiko Matsumoto, Kazuo Hanawa, Shinzi Ogasawara
Publikováno v:
Tetrahedron. 64:3578-3588
Incorporation of modified nucleosides with a flexible universal linker is of great value for post-synthetic modification of nucleic acids. Thus, C8-alkynyl- and alkylamino substituted 2′-deoxyguanosines were synthesized for the first time and incor
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4a2f2103ccce890914947991fc06cc1b
https://doi.org/10.1016/j.tet.2008.01.091
https://doi.org/10.1016/j.tet.2008.01.091
Autor:
Masatoshi Hasegawa, Hiroaki Ozaki, Toshiyuki Kasamatsu, Rina Kitagata, Kazuo Hanawa, Hiroaki Sawai, Shin-ichi Hososhima, Masayasu Kuwahara, Jun-ichi Nagashima, Takehiro Tamura
Publikováno v:
Nucleic Acids Research
We synthesized C5-modified analogs of 2′-deoxyuridine triphosphate and 2′-deoxycytidine triphosphate and investigated them as substrates for PCRs using Taq, Tth, Vent(exo-), KOD Dash and KOD(exo-) polymerases and pUC 18 plasmid DNA as a template.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f6dbb88b65f928f2f6b4974fc505cbe6
https://doi.org/10.1093/nar/gkl637
https://doi.org/10.1093/nar/gkl637
Autor:
Chikara Dohno, Subhendu Sekhar Bag, Kaori Motegi, Kazuo Hanawa, Yoshio Saito, Akimitsu Okamoto, Tomohisa Ichiba, Isao Saito, Kazuki Tainaka, Keigo Hayashi
Publikováno v:
Pure and Applied Chemistry. 78:2305-2312
There is increasing interest in single nucleotide polymorphism (SNP) typing since they can be used as markers to identify the genes that underlie complex diseases and to realize the full potential of pharmacogenomics in analyzing variable response to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d2e2e633e0739b940d6e17d1e94a2b3b
https://doi.org/10.1351/pac200678122305
https://doi.org/10.1351/pac200678122305
Publikováno v:
Tetrahedron Letters. 46:7605-7608
Novel base-discriminating fluorescent (BDF) nucleoside, 8-fluorescence-labeled adenosine derivative ( 8Py A ), was developed for the detection of thymine base on a target DNA. The BDF nucleoside was incorporated into oligodeoxynucleotides by post-syn
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6565e2318f36d59afda924c1b14a7952
https://doi.org/10.1016/j.tetlet.2005.08.157
https://doi.org/10.1016/j.tetlet.2005.08.157
Publikováno v:
ChemInform. 39
We designed a new type of pyrene-labeled base-discrimination fluorescent (BDF) nucleosides (Py)U, (Py)C, (8Py)A and (MePy)dA, which emitted strong fluorescence only when the bases opposite the BDF base are A, G, T and C, respectively. The DNA probes
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c51d724dcfa362731864345231331cd8
https://doi.org/10.1002/chin.200833236
https://doi.org/10.1002/chin.200833236
Autor:
Kazuomi Ohsawa, Toshiyuki Kasamatsu, Hiroaki Ozaki, Hiroaki Sawai, Kazuo Hanawa, Masayasu Kuwahara, Jun-ichi Nagashima
Publikováno v:
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry. 24(1)
We have screened glutamic acid-binding aptamers from a modified DNA pool containing arginine residues using the method of systematic evolution of ligands by exponential enrichment (SELEX). Thirty-one modified DNA molecules were obtained from the enri
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a28cc0643929a6fe82de4af6ed263d5c
https://pubmed.ncbi.nlm.nih.gov/18187867
https://pubmed.ncbi.nlm.nih.gov/18187867
Publikováno v:
Nucleic acids symposium series (2004). (49)
We designed a new type of pyrene-labeled base-discrimination fluorescent (BDF) nucleosides (Py)U, (Py)C, (8Py)A and (MePy)dA, which emitted strong fluorescence only when the bases opposite the BDF base are A, G, T and C, respectively. The DNA probes
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1e5033d13b683c53b35eec4a7b84d841
https://pubmed.ncbi.nlm.nih.gov/17150679
https://pubmed.ncbi.nlm.nih.gov/17150679
Publikováno v:
Scopus-Elsevier
We have developed novel acridone-labelled BDF probe which showed its potential in recognizing opposite matched base from its target sequence via enhancement of fiuorescence intensity. This probe emit at a longer wavelength than previously reported py
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::523668d440721430d66d311721069a66
https://pubmed.ncbi.nlm.nih.gov/17150877
https://pubmed.ncbi.nlm.nih.gov/17150877
Publikováno v:
Bioorganicmedicinal chemistry letters. 16(24)
Fluorescence response upon hybridization of perylene labeled oligonucleotide probes depends on the microenvironment experienced by the perylene fluorophore. In mismatched duplex ((Per)U-C), enhanced fluorescence was observed while in matched duplex (
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c3a75c84b198746a4aebf79bc2a5d0f3
https://pubmed.ncbi.nlm.nih.gov/17035014
https://pubmed.ncbi.nlm.nih.gov/17035014
Publikováno v:
Bioorganicmedicinal chemistry. 14(8)
We synthesized modified 2'-deoxyuridine triphosphates bearing amino acids at the C5 position and investigated their substrate properties for KOD Dash DNA polymerase during polymerase chain reaction (PCR). PCR using C5-modified dUTP having an amino ac
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d4435800585e243299d5f70dc97560bb
https://pubmed.ncbi.nlm.nih.gov/16359870
https://pubmed.ncbi.nlm.nih.gov/16359870