Výsledky vyhledávání - "Kazunori Sugata"

Akademický článek

Multicolor luciferase assay system: one-step monitoring of multiple gene expressions with a single substrate

Autor: Yoshihiro Nakajima, Takuma Kimura, Kazunori Sugata, Toshiteru Enomoto, Atsushi Asakawa, Hidehiro Kubota, Masaaki Ikeda, Yoshihiro Ohmiya

Publikováno v: BioTechniques, Vol 38, Iss 6, Pp 891-894 (2005)

Reporter assays that use luciferase are widely employed for monitoring cellular events associated with gene expression. In general, firefly luciferase and Renilla luciferase are used for monitoring single gene expression. However, the expression of m

Externí odkaz: https://doaj.org/article/a8cf9d0d08964724a3abc3eb9a986881

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Nucleotide-induced flexibility change in neck linkers of dimeric kinesin as detected by distance Measurements using spin-labeling EPR

Autor: Likai Song, Shoji Ueki, Toshiaki Arata, Kazunori Sugata, Piotr G. Fajer, Motoyoshi Nakamura

Publikováno v: Journal of molecular biology. 386(3)

Using dipolar continuous-wave and pulsed electron paramagnetic resonance methods, we have determined the distribution of the distances between two spin labels placed on the middle of each of the neck linkers of dimeric kinesin. In the absence of micr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::da62688233d4c1238fbbc18f9a4e95eb
https://pubmed.ncbi.nlm.nih.gov/19154743

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Dynamic structures of motor proteins myosin and kinesin, and switch protein troponin as detected by SDSL-ESR

Autor: Ryouhei Narumi, Hiroko Kusuhara, Yukio Yamamoto, Satoshi Yasuda, Motoyoshi Nakamura, Kazunori Sugata, Toshiaki Arata, Tomoki Aihara, Keisuke Ueda, Shoji Ueki

Publikováno v: Journal of electron microscopy. 54

We have studied biological nano-machines, motor and switch proteins operating as supramolecular complexes by electron spin resonance (ESR) and found key features of their molecular movements. In all the systems, the specific movements of elements or

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c53ce597e45b403c0b814dd5d107aac9
https://pubmed.ncbi.nlm.nih.gov/16157641

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Orientation and motion of myosin light chain and troponin in reconstituted muscle fibers as detected by ESR with a new bifunctional spin label

Autor: Toshiaki, Arata, Motoyoshi, Nakamura, Hidenobu, Akahane, Tomoki, Aihara, Shoji, Ueki, Kazunori, Sugata, Hiroko, Kusuhara, Masashi, Morimoto, Yukio, Yamamoto

Publikováno v: Advances in experimental medicine and biology. 538

Using electron spin resonance, we have studied dynamic structures of myosin neck domain and troponin C by site-directed spin labeling. We observed two broad but distinct orientations of a spin label attached specifically to a single cysteine (cys156)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::4e192a9bfb863edb2b4345a89416e172
https://pubmed.ncbi.nlm.nih.gov/15098675

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ESR reveals the mobility of the neck linker in dimeric kinesin

Autor: Toshiaki Arata, Kazunori Sugata, Peter G. Fajer, Motoyoshi Nakamura, Shoji Ueki

Publikováno v: Biochemical and biophysical research communications. 314(2)

Conventional kinesin is a highly processive motor that converts the chemical energy of ATP hydrolysis into the unidirectional motility along microtubules. The processivity is thought to depend on the coordination between ATPase cycles of two motor do

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6477ca1b8004883f2fa6bb972506290b
https://pubmed.ncbi.nlm.nih.gov/14733926

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Orientation and Motion of Myosin Light Chain and Troponin in Reconstituted Muscle Fibers as Detected by ESR with a New Bifunctional Spin Label

Autor: Hiroko Kusuhara, Toshiaki Arata, Masashi Morimoto, Kazunori Sugata, Hidenobu Akahane, Tomoki Aihara, Yukio Yamamoto, Motoyoshi Nakamura, Shoji Ueki

Publikováno v: Advances in Experimental Medicine and Biology ISBN: 9781461347644

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::2bb8c09b50513151ab3a9a47c59e4100
https://doi.org/10.1007/978-1-4419-9029-7_26

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