Zobrazeno 1 - 10
of 68
pro vyhledávání: '"Kazunari Yoneda"'
Publikováno v:
FEBS Open Bio, Vol 11, Iss 7, Pp 1981-1986 (2021)
In this study, we investigated the stereospecificity of hydride transfer from NADH to flavin mononucleotide (FMN) in reactions catalyzed by the FMN‐dependent NADH‐indigo reductase expressed by thermophilic Bacillus smithii. We performed 1H‐NMR
Externí odkaz:
https://doaj.org/article/8c91848ee7564377a408ecbb06a1fcb1
Publikováno v:
The Journal of Poultry Science, Vol 49, Iss 2, Pp 111-115 (2012)
This study was conducted to elucidate the effects of dietary phytic acid supplementation on organs and serum components in broiler chickens. A total of 30 1-day-old broiler chicks were divided into three treatment groups (n=10, each). The control gro
Externí odkaz:
https://doaj.org/article/ba46db3ac97b445ca7ba680f6d3839b8
Publikováno v:
FEBS Open Bio
FEBS Open Bio, Vol 11, Iss 7, Pp 1981-1986 (2021)
FEBS Open Bio, Vol 11, Iss 7, Pp 1981-1986 (2021)
In this study, we investigated the stereospecificity of hydride transfer from NADH to flavin mononucleotide (FMN) in reactions catalyzed by the FMN‐dependent NADH‐indigo reductase expressed by thermophilic Bacillus smithii. We performed 1H‐NMR
Publikováno v:
International Journal of Biological Macromolecules. 164:3259-3267
The FMN-dependent NADH-indigo reductase gene from the thermophilic bacterium Bacillus smithii was overexpressed in Escherichia coli. The expressed enzyme functioned as a highly thermostable indigo reductase that retained complete activity even after
Publikováno v:
Proteins: Structure, Function, and Bioinformatics. 88:669-678
A gene encoding galactose 1-phosphate uridylyltransferase (GalT) was identified in the hyperthermophilic archaeon Pyrobaculum aerophilum. The gene was overexpressed in Escherichia coli, after which its product was purified and characterized. The expr
Autor:
Ryushi Kawakami, Tatsuya Ohshida, Junji Hayashi, Kazunari Yoneda, Toshio Furumoto, Toshihisa Ohshima, Haruhiko Sakuraba
Publikováno v:
International journal of biological macromolecules. 208
Ornithine δ-aminotransferase (Orn-AT) activity was detected for the enzyme annotated as a γ-aminobutyrate aminotransferase encoded by PH1423 gene from Pyrococcus horikoshii OT-3. Crystal structures of this novel archaeal ω-aminotransferase were de
Autor:
Haruhiko Sakuraba, Toshihisa Ohshima, Kazunari Yoneda, Kohei Koba, Tatsuya Ohshida, Taketo Ohmori, Junji Hayashi
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 82:2084-2093
The orientation of the three domains in the bifunctional aspartate kinase-homoserine dehydrogenase (AK-HseDH) homologue found in Thermotoga maritima totally differs from those observed in previously known AK-HseDHs; the domains line up in the order H
Publikováno v:
Extremophiles. 22:395-405
A gene encoding L-serine dehydrogenase (L-SerDH) that exhibits extremely low sequence identity to the Agrobacterium tumefaciens L-SerDH was identified in the hyperthermophilic archaeon Pyrobaculum calidifontis. The predicted amino acid sequence showe
Crystal structure of the novel amino-acid racemase isoleucine 2-epimerase fromLactobacillus buchneri
Autor:
Yuta Mutaguchi, Toshihisa Ohshima, Junji Hayashi, Taketo Ohmori, Yume Minemura, Haruhiko Sakuraba, Kazunari Yoneda, Noriko Nakagawa
Publikováno v:
Acta Crystallographica Section D Structural Biology. 73:428-437
Crystal structures ofLactobacillus buchneriisoleucine 2-epimerase, a novel branched-chain amino-acid racemase, were determined for the enzyme in the apo form, in complex with pyridoxal 5′-phosphate (PLP), in complex withN-(5′-phosphopyridoxyl)-L-
Publikováno v:
The Protein Journal. 36:98-107
Superoxide dismutase (SOD) is an antioxidant enzyme protecting cells from oxidative stress. Ginger (Zingiber officinale) is known for its antioxidant properties, however, there are no data on SODs from ginger rhizomes. In this study, we purified SOD