Zobrazeno 1 - 6
of 6
pro vyhledávání: '"Kazuki Nakamae"'
Autor:
Shota Nakade, Keiji Mochida, Atsushi Kunii, Kazuki Nakamae, Tomomi Aida, Kohichi Tanaka, Naoaki Sakamoto, Tetsushi Sakuma, Takashi Yamamoto
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-10 (2018)
Genome editing using CRISPR can be enhanced by manipulating DNA double-strand break repair pathways. Here the authors demonstrate LoAD, local accumulation of repair molecules, which shifts repair to microhomology-mediated end-joining.
Externí odkaz:
https://doaj.org/article/7714049adc384aa7a9920f7849997032
Autor:
Kazuki Nakamae, Mitsumasa Takenaga, Shota Nakade, Akinori Awazu, Naoaki Sakamoto, Takashi Yamamoto, Tetsushi Sakuma
Highly efficient gene knock-out and knock-in have been achieved by harnessing CRISPR-Cas9 and its advanced technologies such as Prime Editor. In addition, various bioinformatics resources have become available to quantify and qualify the efficiency a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e6022d52c3e9490508f564ba3da56e17
https://doi.org/10.21203/rs.3.rs-2772600/v1
https://doi.org/10.21203/rs.3.rs-2772600/v1
Autor:
Hidemasa Bono, Kazuki Nakamae
The CRISPR-Cas9 system has successfully achieved site-specific gene editing in organisms ranging from humans to bacteria. The technology efficiently generates mutants, allowing for phenotypic analysis of the on-target gene. However, some conventional
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::33f1adcd480040d5b6e45413ef42f06b
https://doi.org/10.1101/2023.03.11.531115
https://doi.org/10.1101/2023.03.11.531115
Autor:
Shota Nakade, Kazuki Nakamae, Tzu-Chieh Tang, Dou Yu, Tetsushi Sakuma, Takashi Yamamoto, Timothy K. Lu
Efficiency and accuracy are paramount in genome editing. While CRISPR-Cas nucleases are efficient at editing target genes, their accuracy is limited because following DNA cleavage by Cas proteins, error-prone repair mechanisms introduce random mutati
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6a104afa9e3c5510d2067a85229dd613
https://doi.org/10.1101/2022.12.05.518807
https://doi.org/10.1101/2022.12.05.518807
Autor:
Hidemasa Bono, Kazuki Nakamae
Publikováno v:
Gene and Genome Editing. :100018
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::59ba0459e46fd0d3f2473f1ddf3f8666
https://doi.org/10.1016/j.ggedit.2022.100018
https://doi.org/10.1016/j.ggedit.2022.100018
Autor:
Hiroshi Ide, Mitsumasa Takenaga, Takashi Yamamoto, Naoaki Sakamoto, Tetsushi Sakuma, Kazuki Nakamae, Shota Nakade, Yuki Nishimura
Publikováno v:
Bioengineered
The emerging genome editing technology has enabled the creation of gene knock-in cells easily, efficiently, and rapidly, which has dramatically accelerated research in the field of mammalian functional genomics, including in humans. We recently devel
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::93d8760cf4f6764e370e2859f02de761
https://pubmed.ncbi.nlm.nih.gov/28453368
https://pubmed.ncbi.nlm.nih.gov/28453368