Zobrazeno 1 - 10
of 204
pro vyhledávání: '"Kazuhito Fujiyama"'
Publikováno v:
PLoS ONE, Vol 19, Iss 3, p e0299403 (2024)
N-linked glycosylation is a pivotal post-translational modification that significantly influences various aspects of protein biology. Autophagy, a critical cellular process, is instrumental in cell survival and maintenance. The hepatitis B virus (HBV
Externí odkaz:
https://doaj.org/article/0652028069cc4acea0d1f257af5d8e85
Autor:
Hiroyuki Kajiura, Ken-ichiro Tatematsu, Tsuyoshi Nomura, Mitsuhiro Miyazawa, Akihiro Usami, Toshiki Tamura, Hideki Sezutsu, Kazuhito Fujiyama
Publikováno v:
Scientific Reports, Vol 12, Iss 1, Pp 1-15 (2022)
Abstract The silkworm, Bombyx mori, is an attractive host for recombinant protein production due to its high expression efficiency, quality, and quantity. Two expression systems have been widely used for recombinant protein production in B. mori: bac
Externí odkaz:
https://doaj.org/article/9b9a16dad20a4bc99efd5c515a91f0db
Autor:
Kim Dua Nguyen, Hiroyuki Kajiura, Ryo Kamiya, Takahiro Yoshida, Ryo Misaki, Kazuhito Fujiyama
Publikováno v:
Frontiers in Plant Science, Vol 14 (2023)
N-glycan engineering has dramatically evolved for the development and quality control of recombinant antibodies. Fc region of IgG contains two N-glycans whose galactose terminals on Fc-glycan have been shown to increase the stability of CH2 domain an
Externí odkaz:
https://doaj.org/article/41a31557575945e393caa5e5815afe6b
Autor:
Hiroyuki Kajiura, Ryousuke Miyauchi, Akemi Kakudo, Takao Ohashi, Ryo Misaki, Kazuhito Fujiyama
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
Abstract N-Glycosylation is one of the most important post-translational protein modifications in eukaryotic cells. Although more than 200 N-glycogenes contributing to N-glycan biosynthesis have been identified and characterized, the information on i
Externí odkaz:
https://doaj.org/article/66dc3cfbc89c474a86596a4dccf9a055
Autor:
Hiroyuki Kajiura, Takuya Yoshizawa, Yuji Tokumoto, Nobuaki Suzuki, Shinya Takeno, Kanokwan Jumtee Takeno, Takuya Yamashita, Shun-ichi Tanaka, Yoshinobu Kaneko, Kazuhito Fujiyama, Hiroyoshi Matsumura, Yoshihisa Nakazawa
Publikováno v:
Communications Biology, Vol 4, Iss 1, Pp 1-13 (2021)
Kajiura and Yoshizawa et al. identify three new prenyltransferases in the tree Eucommia ulmoides that synthesize exceptionally high molecular weight trans-1,4-polyisoprene (TPI). Through crystal structure and mutational analyses, they identify key re
Externí odkaz:
https://doaj.org/article/0d8d4e20f5c54474bdd6cf4c60bfba98
Publikováno v:
Frontiers in Plant Science, Vol 12 (2021)
Plant cell cultures have emerged as a promising platform for the production of biopharmaceutics due to their cost-effectiveness, safety, ability to control the cultivation, and secrete products into culture medium. However, the use of this platform i
Externí odkaz:
https://doaj.org/article/003a591b60cb433c84cbb38892ee6f9f
Publikováno v:
Frontiers in Plant Science, Vol 12 (2021)
Gaucher disease is an inherited lysosomal storage disorder caused by a deficiency of functional enzyme β-glucocerebrosidase (GCase). Recombinant GCase has been used in enzyme replacement therapy to treat Gaucher disease. Importantly, the terminal ma
Externí odkaz:
https://doaj.org/article/e7ab9fd5b00e428fa6a5ba8d630eb677
Autor:
Sakaoduoen Bunsangiam, Varunya Sakpuntoon, Nantana Srisuk, Takao Ohashi, Kazuhito Fujiyama, Savitree Limtong
Publikováno v:
Mycobiology, Vol 47, Iss 3, Pp 292-300 (2019)
IAA biosynthetic pathways in a basidiomycetous yeast, Rhodosporidiobolus fluvialis DMKU-CP293, were investigated. The yeast strain showed tryptophan (Trp)-dependent IAA biosynthesis when grown in tryptophan supplemented mineral salt medium. Gas chrom
Externí odkaz:
https://doaj.org/article/79a80917ad644bee9f2f18bf4b08ab4b
Autor:
Takuhiro Matsumura, Sho Amatsu, Ryo Misaki, Masahiro Yutani, Anariwa Du, Tomoko Kohda, Kazuhito Fujiyama, Kazuyoshi Ikuta, Yukako Fujinaga
Publikováno v:
Toxins, Vol 12, Iss 5, p 302 (2020)
Botulinum neurotoxin (BoNT) is the most potent natural toxin known. Of the seven BoNT serotypes (A to G), types A, B, E, and F cause human botulism. Treatment of human botulism requires the development of effective toxin-neutralizing antibodies witho
Externí odkaz:
https://doaj.org/article/90ba26ceb70d4313a0e0fdf0730c4938
Autor:
Sugata Roychowdhury, Young J. Oh, Hiroyuki Kajiura, Krystal T. Hamorsky, Kazuhito Fujiyama, Nobuyuki Matoba
Publikováno v:
Frontiers in Plant Science, Vol 9 (2018)
Nicotiana benthamiana transient overexpression systems offer unique advantages for rapid and scalable biopharmaceuticals production, including high scalability and eukaryotic post-translational modifications such as N-glycosylation. High-mannose-type
Externí odkaz:
https://doaj.org/article/b0ac2cb3e51947849ce83560066ec604