Zobrazeno 1 - 10
of 76
pro vyhledávání: '"Kazufusa Shinomiya"'
Publikováno v:
BioTechniques, Vol 73, Iss 6, Pp 281-287 (2022)
This study sought to develop a short DNA detection method using a deoxyuridine probe and polymerase chain reaction. The probe was hybridized to the target short DNA, which was then extended by DNA polymerase. The extended DNA was used for real-time P
Externí odkaz:
https://doaj.org/article/5d89c9131e0d4518a724bc4c0a12bf1d
Publikováno v:
Biomedical chromatography : BMC.
The development of countercurrent chromatography (CCC) technology enabled to perform higher peak resolutions and more shortened separation times even for protein separation using aqueous two-phase solvent systems composed of polyethylene glycol (PEG)
Autor:
Yoichiro Ito, Susumu Kitanaka, Kazufusa Shinomiya, Risako Koga, Riku Motegi, Kazumasa Zaima, Hanae Sato
Publikováno v:
CHROMATOGRAPHY. 41:91-95
Autor:
Tomomi Hashimoto, Yoichiro Ito, Kazumasa Zaima, Kazufusa Shinomiya, Tomoya Takeda, Ruina Watanabe, Koji Tokura
Publikováno v:
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry. 37(2)
The partition efficiency of the double-spaced coil for eccentric and toroidal coils on countercurrent chromatographic separation of proteins was evaluated using the small-scale cross-axis coil planet centrifuge (CPC) equipped with circular and ellipt
Autor:
Naoki Harikai, Yoichiro Ito, Yukina Harada, Kazufusa Shinomiya, Koji Tokura, Miho Yasue, Kazumasa Zaima
Publikováno v:
Journal of Chromatography A. 1481:64-72
Coil satellite centrifuge (CSC) produces the complex satellite motion consisting of the triplicate rotation of the coiled column around three axes including the sun axis (the angular velocity, ω1), the planet axis (ω2) and the satellite axis (the c
Autor:
Naoki Harikai, Naoki Sugimoto, Yusai Ito, Kyoko Ishizuki, Hiroshi Akiyama, Kazufusa Shinomiya
Publikováno v:
CHEMICAL & PHARMACEUTICAL BULLETIN. 65:883-887
Cochineal extract prepared from the scale insect Dactylopus coccus (American cochineal) has been used as a natural red dye for food, cosmetics, and pharmaceuticals. The major pigment in cochineal extract is carminic acid (CA), an anthraquinone glucos
Autor:
Yoshiyuki Sakuma, Rika Honda, Kazumasa Zaima, Naoki Harikai, Miho Yasue, Yoichiro Ito, Kazufusa Shinomiya, Koji Tokura
Publikováno v:
J Chromatogr A
The partition efficiencies of three different coiled columns, conventional multilayer coiled column, eccentric coiled column and toroidal coiled column, were evaluated by the separation of 4-methylumbelliferyl sugar derivatives using the coil satelli
Autor:
Kazufusa Shinomiya, Etsuhiro Tsukidate, Yoichiro Ito, Kazuhiro Yanagidaira, Kazunori Yoshida, Koji Tokura
Publikováno v:
Analytical Sciences. 31:211-218
Protein separation was performed using the high-speed counter-current chromatograph (HSCCC) at both synchronous and nonsynchronous type-J planetary motions. The partition efficiency was evaluated with two different column configurations, eccentric co
Autor:
Naoki Sugimoto, Hiroshi Akiyama, Yusai Ito, Naoki Harikai, Ryoko Yagi, Kazumasa Zaima, Azusa Fukamachi, Kazufusa Shinomiya
Publikováno v:
Chemicalpharmaceutical bulletin. 65(3)
Carminic acid (CA) is a major component of cochineal dye used in food additives, cosmetics, and pharmaceuticals. CA and its isomers, 2-C-α-glucofuranoside and 2-C-β-glucofuranoside of kermesic acid (DCIV and DCVII, respectively), were isolated from
Autor:
Kazufusa Shinomiya, Naoki Harikai
Publikováno v:
Food Analytical Methods. 8:1215-1224
To improve the efficiency of mitochondrial DNA (mtDNA) extraction from food, a simple method, PrepM, was developed by effectively combining alkaline sodium dodecyl sulfate lysis and DNA binding to a silica membrane. PrepM was evaluated using fresh, c