Zobrazeno 1 - 10 of 85 pro vyhledávání: '"Kazuchika MIYOSHI"'
1
Akademický článek
Cryopreservation of pig spermatozoa using carboxylated poly-L-lysine as cryoprotectant
Autor: Hansol JIN, Wooyoon CHOI, Kazuaki MATSUMURA, Suong-hyu HYON, Yuki GEN, Masakazu HAYASHI, Tadahiro KAWABATA, Moe IJIRI, Kazuchika MIYOSHI
Publikováno v: The Journal of Reproduction and Development, Vol 68, Iss 5, Pp 312-317 (2022)
In this study, we cryopreserved pig spermatozoa using carboxylated poly-L-lysine (CPLL) as the cryoprotectant to determine its efficacy. Pig spermatozoa were placed in a freezing extender containing 3% (v/v) glycerol and different CPLL concentrations
Externí odkaz: https://doaj.org/article/46542be5fde54f1a863e6bae1c1d54c4
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2
Akademický článek
Production of Cloned Miniature Pigs Expressing High Levels of Human Apolipoprotein(a) in Plasma.
Autor: Masayuki Ozawa, Takehiro Himaki, Shoji Ookutsu, Yamato Mizobe, Junki Ogawa, Kazuchika Miyoshi, Akira Yabuki, Jianglin Fan, Mitsutoshi Yoshida
Publikováno v: PLoS ONE, Vol 10, Iss 7, p e0132155 (2015)
High lipoprotein(a) [Lp(a)] levels are a major risk factor for the development of atherosclerosis. However, because apolipoprotein(a) [apo(a)], the unique component of Lp(a), is found only in primates and humans, the study of human Lp(a) has been ham
Externí odkaz: https://doaj.org/article/e02456769c3245939689032a4d63f2ae
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3
Cytoplasmic Microinjection of piggyBac Transposase mRNA and Transposon Vectors for Efficient In Vitro Production of Transgenic Porcine Parthenotes
Autor: Rico Miyagasako, Jin Hansol, Satoshi Watanabe, Kazuchika Miyoshi, Emi Inada, Masahiro Sato
Publikováno v: OBM Genetics. :1-20
The efficient production of transgenic (Tg) piglets has remained a challenge in the field of domestic animal studies. Unlike mice, the pronuclei of pig zygotes cannot be easily studied because of the abundance of lipid droplets. Therefore, the zygote
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::70e4e9abf8c7d315b60dbd78e2d96e51
https://doi.org/10.21926/obm.genet.2203166
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5
Akademický článek
Efficient Generation of Somatic Cell Nuclear Transfer-Competent Porcine Cells with Mutated Alleles at Multiple Target Loci by Using CRISPR/Cas9 Combined with Targeted Toxin-Based Selection System.
Autor: Masahiro Sato1 masasato@m.kufm.kagoshima-u.ac.jp, Kazuchika Miyoshi2 kmiyoshi@agri.kagoshima-u.ac.jp, Shingo Nakamura3 snaka@ndmc.ac.jp, Masato Ohtsuka4,5 masato@is.icc.u-tokai.ac.jp, Takayuki Sakurai6 tsakurai@shinshu-u.ac.jp, Satoshi Watanabe7 kettle@affrc.go.jp, Hiroaki Kawaguchi8 k3038952@kadai.jp, Akihide Tanimoto9 akit09@m3.kufm.kagoshima-u.ac.jp
Publikováno v: International Journal of Molecular Sciences. Dec2017, Vol. 18 Issue 12, p2610. 24p.
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6
In vitro Electroporation in the Presence of CRISPR/Cas9 Reagents as a Safe and Effective Method for Producing Biallelic Knock-Out Porcine Embryos
Autor: Eri Akasaka, Masahiro Sato, Hansol Jin, Kazuchika Miyoshi
Publikováno v: OBM Genetics. 5
The production of genetically modified (GM) pigs is considered valuable in biomedical research for the development of model animals for various diseases and pigs with resistance against viral infection. The porcine genome may be modified using severa
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::8aa6220e23bf2762928028b14d6fbcc4
https://doi.org/10.21926/obm.genet.2101123
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8
Development of a Culture Medium for Rat 1-Cell Embryos
Autor: Kazuchika Miyoshi
Publikováno v: Journal of Mammalian Ova Research. 33:11-16
Rat 1-cell embryos fertilized in vivo or in vitro show a complete developmental block at the 2- to 4-cell stage in conventional culture media. To develop a culture medium supporting early embryonic development in rats, we examined the effects of some
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::025a20073ce8ea08d31601b4427ba65e
https://doi.org/10.1274/032.033.0104
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9
Efficient Generation of Somatic Cell Nuclear Transfer-Competent Porcine Cells with Mutated Alleles at Multiple Target Loci by Using CRISPR/Cas9 Combined with Targeted Toxin-Based Selection System
Autor: Takayuki Sakurai, Masato Ohtsuka, Akihide Tanimoto, Hiroaki Kawaguchi, Satoshi Watanabe, Shingo Nakamura, Masahiro Sato, Kazuchika Miyoshi
Publikováno v: International Journal of Molecular Sciences; Volume 18; Issue 12; Pages: 2610
International Journal of Molecular Sciences
International Journal of Molecular Sciences, Vol 18, Iss 12, p 2610 (2017)
The recent advancement in genome editing such a CRISPR/Cas9 system has enabled isolation of cells with knocked multiple alleles through a one-step transfection. Somatic cell nuclear transfer (SCNT) has been frequently employed as one of the efficient
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::54f77c624f8c660b0064c0182d768b9d
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10
Preincubation with green tea polyphenol extract is beneficial for attenuating sperm injury caused by freezing-thawing in swine
Autor: Masahiro Sato, Hideki Kitaji, Kazuchika Miyoshi, Shoji Ookutsu
Publikováno v: Animal Science Journal. 86:922-928
Polyphenols (PFs) extracted from green tea, known to be potent anti-oxidants, have been reported to be effective in increasing the motility and viability of mammalian sperm, preserved in a liquid form. Therefore, we tested whether PFs might also be e
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::74be0df1c6cf1fe5c15508087bdf2e82
https://doi.org/10.1111/asj.12379
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