Zobrazeno 1 - 10
of 105
pro vyhledávání: '"Kazuchika MIYOSHI"'
Autor:
Hansol JIN, Wooyoon CHOI, Kazuaki MATSUMURA, Suong-hyu HYON, Yuki GEN, Masakazu HAYASHI, Tadahiro KAWABATA, Moe IJIRI, Kazuchika MIYOSHI
Publikováno v:
The Journal of Reproduction and Development, Vol 68, Iss 5, Pp 312-317 (2022)
In this study, we cryopreserved pig spermatozoa using carboxylated poly-L-lysine (CPLL) as the cryoprotectant to determine its efficacy. Pig spermatozoa were placed in a freezing extender containing 3% (v/v) glycerol and different CPLL concentrations
Externí odkaz:
https://doaj.org/article/46542be5fde54f1a863e6bae1c1d54c4
Publikováno v:
OBM Genetics. :1-20
The efficient production of transgenic (Tg) piglets has remained a challenge in the field of domestic animal studies. Unlike mice, the pronuclei of pig zygotes cannot be easily studied because of the abundance of lipid droplets. Therefore, the zygote
Autor:
Hansol Jin, Wooyoon Choi, Kazuaki Matsumura, Suong‐hyu Hyon, Yuki Gen, Masakazu Hayashi, Tadahiro Kawabata, Moe Ijiri, Kazuchika Miyoshi
Publikováno v:
Animal Science Journal. 94
Autor:
Masahiro Sato1 masasato@m.kufm.kagoshima-u.ac.jp, Kazuchika Miyoshi2 kmiyoshi@agri.kagoshima-u.ac.jp, Shingo Nakamura3 snaka@ndmc.ac.jp, Masato Ohtsuka4,5 masato@is.icc.u-tokai.ac.jp, Takayuki Sakurai6 tsakurai@shinshu-u.ac.jp, Satoshi Watanabe7 kettle@affrc.go.jp, Hiroaki Kawaguchi8 k3038952@kadai.jp, Akihide Tanimoto9 akit09@m3.kufm.kagoshima-u.ac.jp
Publikováno v:
International Journal of Molecular Sciences. Dec2017, Vol. 18 Issue 12, p2610. 24p.
Autor:
Masayuki Ozawa, Takehiro Himaki, Shoji Ookutsu, Yamato Mizobe, Junki Ogawa, Kazuchika Miyoshi, Akira Yabuki, Jianglin Fan, Mitsutoshi Yoshida
Publikováno v:
PLoS ONE, Vol 10, Iss 7, p e0132155 (2015)
High lipoprotein(a) [Lp(a)] levels are a major risk factor for the development of atherosclerosis. However, because apolipoprotein(a) [apo(a)], the unique component of Lp(a), is found only in primates and humans, the study of human Lp(a) has been ham
Externí odkaz:
https://doaj.org/article/e02456769c3245939689032a4d63f2ae
Publikováno v:
OBM Genetics. 5
The production of genetically modified (GM) pigs is considered valuable in biomedical research for the development of model animals for various diseases and pigs with resistance against viral infection. The porcine genome may be modified using severa
Autor:
Kazuchika Miyoshi
Publikováno v:
Journal of Mammalian Ova Research. 33:11-16
Rat 1-cell embryos fertilized in vivo or in vitro show a complete developmental block at the 2- to 4-cell stage in conventional culture media. To develop a culture medium supporting early embryonic development in rats, we examined the effects of some
Publikováno v:
Animal Science Journal. 86:922-928
Polyphenols (PFs) extracted from green tea, known to be potent anti-oxidants, have been reported to be effective in increasing the motility and viability of mammalian sperm, preserved in a liquid form. Therefore, we tested whether PFs might also be e
Autor:
Miyu Koriyama, Issei Saitoh, Masahiro Sato, Emi Inada, Takayuki Sakurai, Satoshi Watanabe, Masato Ohtsuka, Kazuchika Miyoshi, Shingo Nakamura
Publikováno v:
International Journal of Molecular Sciences
International Journal of Molecular Sciences, Vol 16, Iss 8, Pp 17838-17856 (2015)
International Journal of Molecular Sciences, Vol 16, Iss 8, Pp 17838-17856 (2015)
Some reports demonstrated successful genome editing in pigs by one-step zygote microinjection of mRNA of CRISPR/Cas9-related components. Given the relatively long gestation periods and the high cost of housing, the establishment of a single blastocys